UUAT1_ARATH
ID UUAT1_ARATH Reviewed; 309 AA.
AC Q9FYE5; Q8W4M4;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=UDP-URONIC ACID TRANSPORTER 1 {ECO:0000303|PubMed:28062750};
GN Name=UUAT1 {ECO:0000303|PubMed:28062750};
GN OrderedLocusNames=At5g04160 {ECO:0000312|Araport:AT5G04160};
GN ORFNames=F21E1.80 {ECO:0000312|EMBL:CAC05498.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=25053812; DOI=10.1073/pnas.1406073111;
RA Rautengarten C., Ebert B., Moreno I., Temple H., Herter T., Link B.,
RA Donas-Cofre D., Moreno A., Saez-Aguayo S., Blanco F., Mortimer J.C.,
RA Schultink A., Reiter W.D., Dupree P., Pauly M., Heazlewood J.L.,
RA Scheller H.V., Orellana A.;
RT "The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter
RT family of Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11563-11568(2014).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, BIOPHYSICOCHEMICAL
RP PROPERTIES, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=28062750; DOI=10.1105/tpc.16.00465;
RA Saez-Aguayo S., Rautengarten C., Temple H., Sanhueza D., Ejsmentewicz T.,
RA Sandoval-Ibanez O., Donas D., Parra-Rojas J.P., Ebert B., Lehner A.,
RA Mollet J.C., Dupree P., Scheller H.V., Heazlewood J.L., Reyes F.C.,
RA Orellana A.;
RT "UUAT1 is a Golgi-localized UDP-uronic acid transporter that modulates the
RT polysaccharide composition of Arabidopsis seed mucilage.";
RL Plant Cell 29:129-143(2017).
CC -!- FUNCTION: UDP-glucuronic acid transporter that modulates the
CC polysaccharide composition of seed mucilage. Transports UDP-glucuronic
CC acid (UDP-GlcA) and UDP-galacturonic acid (UDP-GalA) in vitro.
CC {ECO:0000269|PubMed:28062750}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=1.5 mM for UDP-GlcA {ECO:0000269|PubMed:28062750};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:28062750}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:28062750}.
CC -!- DEVELOPMENTAL STAGE: Expressed during seed development at the mucilage
CC production stages. {ECO:0000269|PubMed:28062750}.
CC -!- DISRUPTION PHENOTYPE: Changes in cell wall monosaccharide composition
CC with a strong reduction of galacturonic acid (GalA), rhamnose and
CC xylose content in the sead coat mucilage composition.
CC {ECO:0000269|PubMed:28062750}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. TPT (TC 2.A.7.9)
CC subfamily. {ECO:0000305}.
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DR EMBL; AL391716; CAC05498.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90706.1; -; Genomic_DNA.
DR EMBL; AY062475; AAL32553.1; -; mRNA.
DR EMBL; AY093253; AAM13252.1; -; mRNA.
DR EMBL; AY084240; AAM60836.1; -; mRNA.
DR RefSeq; NP_196036.1; NM_120498.3.
DR AlphaFoldDB; Q9FYE5; -.
DR SMR; Q9FYE5; -.
DR BioGRID; 15574; 4.
DR IntAct; Q9FYE5; 4.
DR STRING; 3702.AT5G04160.1; -.
DR PaxDb; Q9FYE5; -.
DR PRIDE; Q9FYE5; -.
DR ProteomicsDB; 228667; -.
DR EnsemblPlants; AT5G04160.1; AT5G04160.1; AT5G04160.
DR GeneID; 830294; -.
DR Gramene; AT5G04160.1; AT5G04160.1; AT5G04160.
DR KEGG; ath:AT5G04160; -.
DR Araport; AT5G04160; -.
DR TAIR; locus:2146683; AT5G04160.
DR eggNOG; KOG1441; Eukaryota.
DR HOGENOM; CLU_022332_2_1_1; -.
DR InParanoid; Q9FYE5; -.
DR OMA; TLITFWY; -.
DR OrthoDB; 938634at2759; -.
DR PhylomeDB; Q9FYE5; -.
DR PRO; PR:Q9FYE5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FYE5; baseline and differential.
DR Genevisible; Q9FYE5; AT.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0048359; P:mucilage metabolic process involved in seed coat development; IMP:TAIR.
DR GO; GO:0015780; P:nucleotide-sugar transmembrane transport; IMP:TAIR.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR Pfam; PF03151; TPT; 1.
PE 1: Evidence at protein level;
KW Golgi apparatus; Membrane; Pyruvate; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..309
FT /note="UDP-URONIC ACID TRANSPORTER 1"
FT /id="PRO_0000406115"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..251
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..278
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 200
FT /note="I -> V (in Ref. 3; AAL32553/AAM13252)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 309 AA; 33713 MW; 6835A43410327D8C CRC64;
MSSSAKKQTL FISTLIISWY SSNIGVLLLN KFLLSNYGFK FPIFLTMCHM SACAILSYIS
IVFLKLVPLQ HLKSRSQFLK VATLSIVFCA SVVGGNISLR YLPVSFNQAV GATTPFFTAL
FAYLMTFKRE AWVTYGALVP VVAGVVIASG GEPGFHWFGF IMCISATAAR AFKSVLQGIL
LSSEGEKLNS MNLMLYMSPI AVIALLPVTL FMEPDVISVT LTLAKQHQYM WILLLVNSVM
AYSANLLNFL VTKHTSALTL QVLGNAKGAV AVVISILIFQ NPVTVMGIGG YSITVLGVVA
YGETKRRFR
