UVH3_ARATH
ID UVH3_ARATH Reviewed; 1479 AA.
AC Q9ATY5; Q9LRT2;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=DNA repair protein UVH3;
DE EC=3.1.-.-;
DE AltName: Full=ERCC5 homolog;
DE AltName: Full=RAD2 homolog;
DE Short=AtRAD2;
DE Short=AtUVH3;
DE Short=AtXPG;
DE AltName: Full=UV hypersensitive protein 3;
DE AltName: Full=XPG homolog;
GN Name=UVH3; Synonyms=RAD2, XPG; OrderedLocusNames=At3g28030;
GN ORFNames=MMG15.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=11439121; DOI=10.1046/j.1365-313x.2001.01031.x;
RA Liu Z., Hall J.D., Mount D.W.;
RT "Arabidopsis UVH3 gene is a homolog of the Saccharomyces cerevisiae RAD2
RT and human XPG DNA repair genes.";
RL Plant J. 26:329-338(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: Putative single-stranded DNA endonuclease involved in
CC nucleotide excision repair (NER) of UV- and oxidative damaged DNA. May
CC make the 3'-incision step in NER. Seems to play a role in senescence
CC program. {ECO:0000269|PubMed:11439121}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 2 magnesium ions per subunit. They probably participate in
CC the reaction catalyzed by the enzyme. May bind an additional third
CC magnesium ion after substrate binding. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in all tissues at low levels.
CC {ECO:0000269|PubMed:11439121}.
CC -!- SIMILARITY: Belongs to the XPG/RAD2 endonuclease family. XPG subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01125.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF312711; AAK37472.1; -; mRNA.
DR EMBL; AB028616; BAB01125.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; Q9ATY5; -.
DR SMR; Q9ATY5; -.
DR BioGRID; 7757; 2.
DR IntAct; Q9ATY5; 1.
DR STRING; 3702.AT3G28030.1; -.
DR PaxDb; Q9ATY5; -.
DR PRIDE; Q9ATY5; -.
DR ProteomicsDB; 243242; -.
DR Araport; AT3G28030; -.
DR TAIR; locus:2091343; AT3G28030.
DR eggNOG; KOG2520; Eukaryota.
DR HOGENOM; CLU_003018_1_0_1; -.
DR InParanoid; Q9ATY5; -.
DR PhylomeDB; Q9ATY5; -.
DR PRO; PR:Q9ATY5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9ATY5; baseline and differential.
DR Genevisible; Q9ATY5; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0017108; F:5'-flap endonuclease activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR GO; GO:0010213; P:non-photoreactive DNA repair; IMP:TAIR.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:InterPro.
DR GO; GO:0009408; P:response to heat; IMP:TAIR.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR006086; XPG-I_dom.
DR InterPro; IPR006084; XPG/Rad2.
DR InterPro; IPR001044; XPG/Rad2_eukaryotes.
DR InterPro; IPR019974; XPG_CS.
DR InterPro; IPR006085; XPG_DNA_repair_N.
DR Pfam; PF14377; UBM; 1.
DR Pfam; PF00867; XPG_I; 1.
DR Pfam; PF00752; XPG_N; 1.
DR PRINTS; PR00853; XPGRADSUPER.
DR PRINTS; PR00066; XRODRMPGMNTG.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00484; XPGI; 1.
DR SMART; SM00485; XPGN; 1.
DR SUPFAM; SSF47807; SSF47807; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
DR PROSITE; PS00841; XPG_1; 1.
DR PROSITE; PS00842; XPG_2; 1.
PE 2: Evidence at transcript level;
KW DNA damage; DNA repair; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW Nuclease; Nucleus; Reference proteome.
FT CHAIN 1..1479
FT /note="DNA repair protein UVH3"
FT /id="PRO_0000154034"
FT REGION 1..98
FT /note="N-domain"
FT REGION 128..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 239..266
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 384..425
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 548..579
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 854..877
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 934..1022
FT /note="I-domain"
FT REGION 1206..1372
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1418..1479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 393..410
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1219..1259
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1260..1279
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1325..1359
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1423..1453
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 30
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 77
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 946
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 948
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 967
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 969
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 1018
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
SQ SEQUENCE 1479 AA; 165668 MW; 72F38D472849EE55 CRC64;
MGVQGLWELL APVGRRVSVE TLANKRLAID ASIWMVQFIK AMRDEKGDMV QNAHLIGFFR
RICKLLFLRT KPIFVFDGAT PALKRRTVIA RRRQRENAQT KIRKTAEKLL LNRLKDIRLK
EQAKDIKNQR LKQDDSDRVK KRVSSDSVED NLRVPVEEDD VGASFFQEEK LDEVSQASLV
GETGVDDVVK ESVKDDPKGK GVLLDGDDLD NLVQDSSVQG KDYQEKLDEM LAASLAAEEE
RNFTSKASTS AAAIPSEEDE EEDSDGDEEI LLPVMDGNID PAVLASLPPS MQLDLLAQMR
EKLMAENRQK YQKVKKAPEK FSELQIEAYL KTVAFRREIN EVQRSAGGRA VGGVQTSRIA
SEANREFIFS SSFAGDKEVL ASAREGRNDE NQKKTSQQSL PVSVKNASPL KKSDATIELD
RDEPKNPDEN IEVYIDERGR FRIRNRHMGI QMTRDIQRNL HLMKEKERTA SGSMAKNDET
FSAWENFPTE DQFLEKSPVE KDVVDLEIQN DDSMLHPPSS IEISFDHDGG GKDLNDEDDM
FLQLAAGGPV TISSTENDPK EDTSPWASDS DWEEVPVEQN TSVSKLEANL SNQHIPKDIS
IAEGVAWEEY SCKNANNSVE NDTVTKITKG YLEEEADLQE AIKKSLLELH DKESGDVLEE
NQSVRVNLVV DKPSEDSLCS RETVGEAEEE RFLDEITILK TSGAISEQSN TSVAGNADGQ
KGITKQFGTH PSSGSNNVSH AVSNKLSKVK SVISPEKALN VASQNRMLST MAKQHNEEGS
ESFGGESVKV SAMPIADEEI TGFLDEKDNA DGESSIMMDD KRDYSRRKIQ SLVTESRDPS
RNVVRSRIGI LHDTDSQNER REENNSNEHT FNIDSSTDFE EKGVPVEFSE ANIEEEIRVL
DQEFVSLGDE QRKLERNAES VSSEMFAECQ ELLQIFGIPY IIAPMEAEAQ CAFMEQSNLV
DGIVTDDSDV FLFGARSVYK NIFDDRKYVE TYFMKDIEKE LGLSRDKIIR MAMLLGSDYT
EGISGIGIVN AIEVVTAFPE EDGLQKFREW VESPDPTILG KTDAKTGSKV KKRGSASVDN
KGIISGASTD DTEEIKQIFM DQHRKVSKNW HIPLTFPSEA VISAYLNPQV DLSTEKFSWG
KPDLSVLRKL CWEKFNWNGK KTDELLLPVL KEYEKRETQL RIEAFYSFNE RFAKIRSKRI
NKAVKGIGGG LSSDVADHTL QEGPRKRNKK KVAPHETEDN NTSDKDSPIA NEKVKNKRKR
LEKPSSSRGR GRAQKRGRGR GRVQKDLLEL SDGSSDDDDD DDKVVELEAK PANLQKSTRS
RNPVMYSAKE DDELDESRSN EGSPSENFEE VDEGRIGNDD SVDASINDCP SEDYIQTGGG
FCADEADEIG DAHLEDKATD DYRVIGGGFC VDEDETAEEN TMDDDAEILK MESEEQRKKG
KRRNEEDASL DENVDIHFGN SSAGGLSAMP FLKRKKRKN
