UVRAG_DROME
ID UVRAG_DROME Reviewed; 696 AA.
AC Q9VK07;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=UV radiation resistance-associated gene protein;
DE AltName: Full=UV-resistance associated gene;
GN Name=Uvrag; ORFNames=CG6116;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214; SER-218; SER-666;
RP TYR-667; SER-668 AND SER-685, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
RN [5]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=21729695; DOI=10.1016/j.ydbio.2011.06.024;
RA Lee G., Liang C., Park G., Jang C., Jung J.U., Chung J.;
RT "UVRAG is required for organ rotation by regulating Notch endocytosis in
RT Drosophila.";
RL Dev. Biol. 356:588-597(2011).
CC -!- FUNCTION: Acts as a cell growth regulator. Also has a crucial role in
CC controlling organ rotation by regulating membrane-localized Notch
CC receptor endocytosis and subsequent degradation. Regulation of organ
CC rotation is not by induction of autophagy.
CC {ECO:0000269|PubMed:21729695}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development, levels
CC increasing from embryo to pupa, then decreasing in adults with higher
CC levels in adult males than females. {ECO:0000269|PubMed:21729695}.
CC -!- DISRUPTION PHENOTYPE: Homozygous larval lethal. Adult viable flies
CC exhibit genitalia-specific organ rotation phenotypes.
CC {ECO:0000269|PubMed:21729695}.
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DR EMBL; AE014134; AAF53277.1; -; Genomic_DNA.
DR EMBL; AY069353; AAL39498.1; -; mRNA.
DR RefSeq; NP_609632.1; NM_135788.3.
DR AlphaFoldDB; Q9VK07; -.
DR SMR; Q9VK07; -.
DR BioGRID; 60773; 9.
DR STRING; 7227.FBpp0080039; -.
DR iPTMnet; Q9VK07; -.
DR PaxDb; Q9VK07; -.
DR DNASU; 34735; -.
DR EnsemblMetazoa; FBtr0080460; FBpp0080039; FBgn0032499.
DR GeneID; 34735; -.
DR KEGG; dme:Dmel_CG6116; -.
DR UCSC; CG6116-RA; d. melanogaster.
DR CTD; 7405; -.
DR FlyBase; FBgn0032499; Uvrag.
DR VEuPathDB; VectorBase:FBgn0032499; -.
DR eggNOG; KOG2896; Eukaryota.
DR GeneTree; ENSGT00390000012877; -.
DR InParanoid; Q9VK07; -.
DR OrthoDB; 1081121at2759; -.
DR PhylomeDB; Q9VK07; -.
DR Reactome; R-DME-1632852; Macroautophagy.
DR BioGRID-ORCS; 34735; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34735; -.
DR PRO; PR:Q9VK07; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032499; Expressed in saliva-secreting gland and 29 other tissues.
DR ExpressionAtlas; Q9VK07; baseline and differential.
DR Genevisible; Q9VK07; DM.
DR GO; GO:0005768; C:endosome; IBA:GO_Central.
DR GO; GO:0000323; C:lytic vacuole; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0048803; P:imaginal disc-derived male genitalia morphogenesis; IMP:FlyBase.
DR GO; GO:0060575; P:intestinal epithelial cell differentiation; IMP:FlyBase.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:FlyBase.
DR GO; GO:0045746; P:negative regulation of Notch signaling pathway; IGI:FlyBase.
DR GO; GO:0016322; P:neuron remodeling; IMP:FlyBase.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0048260; P:positive regulation of receptor-mediated endocytosis; IMP:FlyBase.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0035493; P:SNARE complex assembly; IBA:GO_Central.
DR InterPro; IPR018791; UV_resistance/autophagy_Atg14.
DR Pfam; PF10186; ATG14; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Growth regulation; Notch signaling pathway; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..696
FT /note="UV radiation resistance-associated gene protein"
FT /id="PRO_0000065749"
FT REGION 666..696
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 251..278
FT /evidence="ECO:0000255"
FT COILED 330..394
FT /evidence="ECO:0000255"
FT COMPBIAS 666..680
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 681..696
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 214
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 218
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 666
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 667
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 668
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 685
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 696 AA; 80690 MW; 2E6FDA367CF8D0CD CRC64;
MNLRPRCRKW LPLATQQLRL RNLNRIQGFN IESWPHEKSL ELDDPDDVLL YYTLHTDKAS
EAFYTSEKLP QRHQQQKWAE ICTDDEAWRK TNAQCVCVKV WKHYSAERRD GQPPEVEQRH
KDVFGRSQLT PSRLPRPPEL LFSWGVYFSG LIPLSPLTLS QCGRNCLVFQ LNGEQFASPS
MISEQALQSQ LHLHYQKYAE EEKLEEPQDE GINSPAVSRS SSPVLRMSTM RYAQLKCQRQ
EIRRSNNLEK LLTLQRLQRL HQQKRRQMAE VCREIARLSV HCVTRNELRL KPRTTSLSGD
YSAHQYHSMG RALSVLLAEQ QQIAPLTLYN AQQLTRRIEA LSSQQRLLKA ERETFRQRNE
RTRQLLKEMR EQREAQQWEL HSQRHRLEKE RLELRTLAPQ HLEQRDQKRQ IERQVERRMS
TLVLELQEIY NIQNVGGRQF SICGIAFPHM EQYTSESRQA ANAQLLDNVS PLAVSAALGY
VAHLVQMLAI IMDRPLRNRI LYEPSKARIV DDIKELTYTT REFPLYTRSI LPSQQTKYAI
YLLRQNVSQL CFDITGQCDL RNTFGNLLEL FSTLRYIERT QRDEVDERDG TAVGAGCGEA
RLANGLTAPH LSQSHSSVDM NHVPLPTGVN AVKDALLQQL LPPGVSEALA IEGYASTQRI
CRSVGSYSDG EDEFRPRLEH NYSNSDSNIT LQTERS
