UVRA_BORBU
ID UVRA_BORBU Reviewed; 950 AA.
AC O51777;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=UvrABC system protein A {ECO:0000255|HAMAP-Rule:MF_00205};
DE Short=UvrA protein {ECO:0000255|HAMAP-Rule:MF_00205};
DE AltName: Full=Excinuclease ABC subunit A {ECO:0000255|HAMAP-Rule:MF_00205};
GN Name=uvrA {ECO:0000255|HAMAP-Rule:MF_00205}; OrderedLocusNames=BB_0837;
OS Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS (Borrelia burgdorferi).
OC Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX NCBI_TaxID=224326;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX PubMed=9403685; DOI=10.1038/37551;
RA Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA Smith H.O., Venter J.C.;
RT "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL Nature 390:580-586(1997).
CC -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein.
CC A damage recognition complex composed of 2 UvrA and 2 UvrB subunits
CC scans DNA for abnormalities. When the presence of a lesion has been
CC verified by UvrB, the UvrA molecules dissociate. {ECO:0000255|HAMAP-
CC Rule:MF_00205}.
CC -!- SUBUNIT: Forms a heterotetramer with UvrB during the search for
CC lesions. {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. UvrA family.
CC {ECO:0000255|HAMAP-Rule:MF_00205}.
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DR EMBL; AE000783; AAC67184.1; -; Genomic_DNA.
DR PIR; D70204; D70204.
DR RefSeq; NP_212971.1; NC_001318.1.
DR RefSeq; WP_010889836.1; NC_001318.1.
DR AlphaFoldDB; O51777; -.
DR SMR; O51777; -.
DR STRING; 224326.BB_0837; -.
DR PRIDE; O51777; -.
DR EnsemblBacteria; AAC67184; AAC67184; BB_0837.
DR KEGG; bbu:BB_0837; -.
DR PATRIC; fig|224326.49.peg.1229; -.
DR HOGENOM; CLU_001370_0_2_12; -.
DR OMA; PFEGIIP; -.
DR Proteomes; UP000001807; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00205; UvrA; 1.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004602; UvrA.
DR InterPro; IPR041552; UvrA_DNA-bd.
DR InterPro; IPR041102; UvrA_inter.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF17755; UvrA_DNA-bind; 1.
DR Pfam; PF17760; UvrA_inter; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR00630; uvra; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA damage; DNA excision; DNA repair; DNA-binding;
KW Excision nuclease; Metal-binding; Nucleotide-binding; Reference proteome;
KW Repeat; SOS response; Zinc; Zinc-finger.
FT CHAIN 1..950
FT /note="UvrABC system protein A"
FT /id="PRO_0000093036"
FT DOMAIN 317..599
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT DOMAIN 619..947
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 260..287
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 750..776
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 36..43
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 651..658
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
SQ SEQUENCE 950 AA; 105796 MW; 03C15EA5CC7195E1 CRC64;
MEKSLKKKII VRGAKEHNLK NVDVDIPKDG LVVISGKSGS GKSSLAFDTI FAEGQRRYME
SVSAYARQFL GVMKKPNVDY IDGLSPSIAI EQRTISNNPR STVGTITEIY DYYRLIFAKI
GKAYCPNDGR LIEEQSLDKI VNTILSYSEG SKVILFAPIV RGSKGSHKKV LEKILNQGFN
RVRINSEDYL IEDALNLNLH KNKKHTIEII VDRIKLGNNV RVRLAESIET SLAVSNGYLR
VEIDNDLEKI DKLFTEHNSC PLCGFSLPLI EPRLFSFNSP FGACSECSGL GVTLEFDFES
ICPDTSLSFN DDAFLTFKTS SSWSVAIFKG LAKHYNFDLN TPIKDIPDKV LKQILYGSNE
KIDFIYQSKE MEAKELDGGF HYSKTFEGLL PLLKRRYLAT ESESTKIFYE NLMSKKICNS
CKGKRLSVGA LTVKINGKDI QDLTNLSVFD SYVFFENLQL DMVDEKISKE ILKEIKSRLK
FLIDVGLSYL YLNRISGSLS GGEAQRIRLA TQIGSALSGV IYVLDEPSIG LHQRDNEKLI
STLVNLKNLG NTVIVVEHDE QTLRTADYII DMGPGAGILG GEIVAKGALI DILNSKNSLT
GQYLSGKFKI DVPSSRRKAD KGEILLLGSN KNNLKNIDLS IPLGVFTVIT GVSGSGKSTL
LNEVLYPALD SRLKLNEKYC DGFKDIVGYE KIDKIIQINQ KPIGRTSRSN PATYVGFFTE
IRELFAKLPD AKSRGFKAGR FSFNVKGGRC EKCQGDGYLN IQMHFLPDVF VPCDLCKGKK
FNEETLEVRY KGKNIHDVLE MSVFEASKFF ENVPKISHYL KFLIEVGLEY IKLGQSATTL
SGGEAQRIKL AFELSKKSTG KTFYIIDEPT TGLHFDDIKK LLEVLQRLVS NGNTVVLIEH
NLDVIKQADY IIDLGPDGGL AGGNIVVSGI PEEVAKCENS YTGMFLKNLL
