UVRA_CLOAB
ID UVRA_CLOAB Reviewed; 939 AA.
AC Q97LQ1;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=UvrABC system protein A {ECO:0000255|HAMAP-Rule:MF_00205};
DE Short=UvrA protein {ECO:0000255|HAMAP-Rule:MF_00205};
DE AltName: Full=Excinuclease ABC subunit A {ECO:0000255|HAMAP-Rule:MF_00205};
GN Name=uvrA {ECO:0000255|HAMAP-Rule:MF_00205}; OrderedLocusNames=CA_C0503;
OS Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710
OS / VKM B-1787).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=272562;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX PubMed=11466286; DOI=10.1128/jb.183.16.4823-4838.2001;
RA Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R.,
RA Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F.,
RA Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V.,
RA Smith D.R.;
RT "Genome sequence and comparative analysis of the solvent-producing
RT bacterium Clostridium acetobutylicum.";
RL J. Bacteriol. 183:4823-4838(2001).
CC -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein.
CC A damage recognition complex composed of 2 UvrA and 2 UvrB subunits
CC scans DNA for abnormalities. When the presence of a lesion has been
CC verified by UvrB, the UvrA molecules dissociate. {ECO:0000255|HAMAP-
CC Rule:MF_00205}.
CC -!- SUBUNIT: Forms a heterotetramer with UvrB during the search for
CC lesions. {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. UvrA family.
CC {ECO:0000255|HAMAP-Rule:MF_00205}.
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DR EMBL; AE001437; AAK78483.1; -; Genomic_DNA.
DR PIR; H96961; H96961.
DR RefSeq; NP_347143.1; NC_003030.1.
DR RefSeq; WP_010963825.1; NC_003030.1.
DR AlphaFoldDB; Q97LQ1; -.
DR SMR; Q97LQ1; -.
DR STRING; 272562.CA_C0503; -.
DR PRIDE; Q97LQ1; -.
DR EnsemblBacteria; AAK78483; AAK78483; CA_C0503.
DR GeneID; 44997012; -.
DR KEGG; cac:CA_C0503; -.
DR PATRIC; fig|272562.8.peg.702; -.
DR eggNOG; COG0178; Bacteria.
DR HOGENOM; CLU_001370_0_2_9; -.
DR OMA; PFEGIIP; -.
DR OrthoDB; 152379at2; -.
DR Proteomes; UP000000814; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00205; UvrA; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004602; UvrA.
DR InterPro; IPR041552; UvrA_DNA-bd.
DR InterPro; IPR041102; UvrA_inter.
DR Pfam; PF17755; UvrA_DNA-bind; 1.
DR Pfam; PF17760; UvrA_inter; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR00630; uvra; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA damage; DNA excision; DNA repair; DNA-binding;
KW Excision nuclease; Metal-binding; Nucleotide-binding; Reference proteome;
KW Repeat; SOS response; Transport; Zinc; Zinc-finger.
FT CHAIN 1..939
FT /note="UvrABC system protein A"
FT /id="PRO_0000093045"
FT DOMAIN 309..588
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT DOMAIN 608..936
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 252..279
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 739..765
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 32..39
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 640..647
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
SQ SEQUENCE 939 AA; 104524 MW; B8B5C1B40548759A CRC64;
MKDKIVIKGA KVHNLKNVDL TVPRNEFVVF TGLSGSGKSS LAFDTLYAEG QRRYMESLSS
YARQFLGQMD KPNVEYIEGL SPAISIDQKT TGRNPRSTVG TVTEIYDYLR LLYAKIGVPH
CPNCGKEISQ QTVDQIVDSV MKLEERTKIQ VLAPVVRGRK GEHTKLIAHI KKSGFVRVRI
DGEVYEIDED EIKLDKNKKH HIEALVDRIV IKQGVEGRLT DSVETALKLA EGLVVINVMG
KEDMLFSENF ACPDCGISIG EISPSMFSFN APFGKCDVCD GIGTLLEIDE DLVIPDKSKS
IMEGAIAPWG EGRLKEESWT FGVLKALSKK YKLDINKPIE DYDEKTLNIL LYGAPDVLKV
NYVKDSQEMV FNHHYEGIIN QMKRRYMESN SDYIKSEIEN YMSNNPCPKC KGARLKKEVL
AITVGNKNIF EFCSMPIREE VSFIDTLELS NKHKIISAQI VKEIKSRLEF LINVGLDYLN
LAREARTLSG GESQRIRLAT QIGSSLVGVL YILDEPSIGL HQRDNDRLIA TMKNLKDIGN
TLIVVEHDED TIKAADFIVD IGPGAGEHGG EIIAAGSLED IKNCKESITG QYLTGVKKIE
VPKERREAGK NFIEIVGAKE NNLKNVNVKF PVGLFTCVTG VSGSGKSTLV NEILYKALNK
KINRSKLNPG KYKSINGIEN IDKIIDINQS PIGRTPRSNP ATYTGVFDII RELYASTKEA
KLRGYKAGRF SFNVKGGRCE ACKGDGIVRI EMQFLSDVYV PCDVCKGKRY NRETLEIKYK
DKNIDDLLNM TVEDALKFFE NLPRIKNKLQ TLADVGLGYV RLGQPSTQLS GGEAQRIKLA
YELSKRSTGK TLYILDEPTT GLHTDDVKKL ISILQRLTDM GNTVVVIEHN LDVIKCADYI
VDLGPEGGEK GGTILCSGTP EQVAQNSSSY TGQYLKKML
