UVRA_DEIRA
ID UVRA_DEIRA Reviewed; 1016 AA.
AC Q46577; O32498;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=UvrABC system protein A {ECO:0000255|HAMAP-Rule:MF_00205};
DE Short=UvrA protein {ECO:0000255|HAMAP-Rule:MF_00205};
DE AltName: Full=Excinuclease ABC subunit A {ECO:0000255|HAMAP-Rule:MF_00205};
GN Name=uvrA {ECO:0000255|HAMAP-Rule:MF_00205}; OrderedLocusNames=DR_1771;
OS Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG
OS 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422).
OC Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae;
OC Deinococcus.
OX NCBI_TaxID=243230;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC 9279 / R1 / VKM B-1422;
RX PubMed=8955293; DOI=10.1128/jb.178.23.6759-6765.1996;
RA Agostini H.J., Carroll J.D., Minton K.W.;
RT "Identification and characterization of uvrA, a DNA repair gene of
RT Deinococcus radiodurans.";
RL J. Bacteriol. 178:6759-6765(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=KD8301;
RX PubMed=9370272; DOI=10.1016/s0378-1119(97)00301-6;
RA Narumi I., Cherdchu K., Kitayama S., Watanabe H.;
RT "The Deinococcus radiodurans uvr A gene: identification of mutation sites
RT in two mitomycin-sensitive strains and the first discovery of insertion
RT sequence element from deinobacteria.";
RL Gene 198:115-126(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC 9279 / R1 / VKM B-1422;
RX PubMed=10567266; DOI=10.1126/science.286.5444.1571;
RA White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D.,
RA Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L.,
RA Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M.,
RA Vamathevan J.J., Lam P., McDonald L.A., Utterback T.R., Zalewski C.,
RA Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D.,
RA Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C.,
RA Fraser C.M.;
RT "Genome sequence of the radioresistant bacterium Deinococcus radiodurans
RT R1.";
RL Science 286:1571-1577(1999).
CC -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein.
CC A damage recognition complex composed of 2 UvrA and 2 UvrB subunits
CC scans DNA for abnormalities. When the presence of a lesion has been
CC verified by UvrB, the UvrA molecules dissociate. {ECO:0000255|HAMAP-
CC Rule:MF_00205}.
CC -!- SUBUNIT: Forms a heterotetramer with UvrB during the search for
CC lesions. {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. UvrA family.
CC {ECO:0000255|HAMAP-Rule:MF_00205}.
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DR EMBL; U52145; AAC44646.1; -; Genomic_DNA.
DR EMBL; AB001610; BAA21368.1; -; Genomic_DNA.
DR EMBL; AE000513; AAF11324.1; -; Genomic_DNA.
DR PIR; H75356; H75356.
DR RefSeq; NP_295494.1; NC_001263.1.
DR RefSeq; WP_010888406.1; NZ_CP015081.1.
DR AlphaFoldDB; Q46577; -.
DR SMR; Q46577; -.
DR STRING; 243230.DR_1771; -.
DR EnsemblBacteria; AAF11324; AAF11324; DR_1771.
DR KEGG; dra:DR_1771; -.
DR PATRIC; fig|243230.17.peg.1983; -.
DR eggNOG; COG0178; Bacteria.
DR HOGENOM; CLU_001370_0_2_0; -.
DR InParanoid; Q46577; -.
DR OMA; PFEGIIP; -.
DR OrthoDB; 152379at2; -.
DR Proteomes; UP000002524; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; -; 3.
DR HAMAP; MF_00205; UvrA; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004602; UvrA.
DR InterPro; IPR041552; UvrA_DNA-bd.
DR InterPro; IPR041102; UvrA_inter.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF17755; UvrA_DNA-bind; 1.
DR Pfam; PF17760; UvrA_inter; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR00630; uvra; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA damage; DNA excision; DNA repair; DNA-binding;
KW Excision nuclease; Metal-binding; Nucleotide-binding; Reference proteome;
KW Repeat; SOS response; Zinc; Zinc-finger.
FT CHAIN 1..1016
FT /note="UvrABC system protein A"
FT /id="PRO_0000093048"
FT DOMAIN 315..627
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT DOMAIN 647..975
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 259..286
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 778..804
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT REGION 984..1016
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 986..1016
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 32..39
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 679..686
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT CONFLICT 282
FT /note="A -> P (in Ref. 1; AAC44646)"
FT /evidence="ECO:0000305"
FT CONFLICT 395..397
FT /note="DTE -> TP (in Ref. 1; AAC44646)"
FT /evidence="ECO:0000305"
FT CONFLICT 470
FT /note="R -> A (in Ref. 1; AAC44646)"
FT /evidence="ECO:0000305"
FT CONFLICT 677
FT /note="V -> E (in Ref. 1; AAC44646)"
FT /evidence="ECO:0000305"
FT CONFLICT 682
FT /note="G -> R (in Ref. 1; AAC44646)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1016 AA; 112100 MW; 9307574FA28676E8 CRC64;
MQDKLIVRGA REHNLKDITV ELPRDRFVVI TGVSGSGKST LAFDTIYAEG QRRYVESLSA
YARQFLGLME KPDVDSITGL SPAISIDQKT TSHNPRSTVG TVTEIHDYLR LLYARVGTPY
CPICGRKIEK QSPSEVTDRL LAGFPDKRAI LLAPAVRGRK GEYKKLFADL RREGYARVRV
DGTLYELEEA EKLKLEKFEK HDVDIVIDRL TLRESDRSRI AESVELGIRR GEGLLRVLLP
DAGEDGGAHE ELYSEKFACP EHGSVLEELE PRSFSFNSPY GACGDCAGIG AKQEFSPERI
IDEKLSIAGG AIIPWTKKGA DAGIYYWDKL KALAEHLDFD LKTPWKDLPA KAQKAVLHGP
GEAFEVVYRR GGKETMRFMT EFEGVITNLE RRYADTESEF MRERLEELME LRPCPTCGGT
RYKPEILAVR VGGLNISQTS GMSVLDADAF FQQLQEGELD HAAIEPFLKR HTGGTAKAHG
PLHYEYDLGT FGAAVAAPIL RAIRTRLKFL VDVGLDYLSL DRTANTLSGG EAQRIRLATQ
VGSGLTGVLY VLDEPSIGLH PKDNGRLIGT LKNLRDLGNS LLVVEHDEDT MLEADYLIDM
GPGAGVHGGE VIASGTPEQV KQDKNSLTGK YLRGEMKIEV PAERRPGNGK FLKVFGARQN
NLQDVDVSIP LGTMTVVTGP SGSGKSTLIH DILHATLARE LNGAKTTPGL YDRIEGMEQL
DKVIEIDQSP IGRTPRSNPA TYTGVFTEIR DLFTRTPEAR RRGYQAGRFS FNVKGGRCEH
CKGDGVMKIE MNFLPDIYVP CEVCHGARYN RETLEVKYNH KTIADVLDLT VEDAHEFFEA
IPTIERKMQL LLDVGLGYMK IGQPSTTLSG GEAQRIKLAT ELSKRATGRT IYILDEPTTG
LHFEDVRKLM DVLQRLAEGG NTLVIIEHNL DVMKSADYLI DLGPEGGVRG GTVVAVGTPE
EVAAHPTSYT GEYLRKVPGI VAAEPRARGE KAEKPAKAKA PAKKRTKKQT ELVEAD
