UVRA_PSEAE
ID UVRA_PSEAE Reviewed; 945 AA.
AC Q9HWG0;
DT 26-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=UvrABC system protein A {ECO:0000255|HAMAP-Rule:MF_00205};
DE Short=UvrA protein {ECO:0000255|HAMAP-Rule:MF_00205};
DE AltName: Full=Excinuclease ABC subunit A {ECO:0000255|HAMAP-Rule:MF_00205};
GN Name=uvrA {ECO:0000255|HAMAP-Rule:MF_00205}; OrderedLocusNames=PA4234;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein.
CC A damage recognition complex composed of 2 UvrA and 2 UvrB subunits
CC scans DNA for abnormalities. When the presence of a lesion has been
CC verified by UvrB, the UvrA molecules dissociate. {ECO:0000255|HAMAP-
CC Rule:MF_00205}.
CC -!- SUBUNIT: Forms a heterotetramer with UvrB during the search for
CC lesions. {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. UvrA family.
CC {ECO:0000255|HAMAP-Rule:MF_00205}.
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DR EMBL; AE004091; AAG07622.1; -; Genomic_DNA.
DR PIR; E83117; E83117.
DR RefSeq; NP_252924.1; NC_002516.2.
DR RefSeq; WP_003093663.1; NZ_QZGE01000028.1.
DR AlphaFoldDB; Q9HWG0; -.
DR SMR; Q9HWG0; -.
DR STRING; 287.DR97_3677; -.
DR PaxDb; Q9HWG0; -.
DR PRIDE; Q9HWG0; -.
DR EnsemblBacteria; AAG07622; AAG07622; PA4234.
DR GeneID; 881827; -.
DR KEGG; pae:PA4234; -.
DR PATRIC; fig|208964.12.peg.4435; -.
DR PseudoCAP; PA4234; -.
DR HOGENOM; CLU_001370_0_2_6; -.
DR InParanoid; Q9HWG0; -.
DR OMA; PFEGIIP; -.
DR PhylomeDB; Q9HWG0; -.
DR BioCyc; PAER208964:G1FZ6-4307-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00205; UvrA; 1.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004602; UvrA.
DR InterPro; IPR041552; UvrA_DNA-bd.
DR InterPro; IPR041102; UvrA_inter.
DR Pfam; PF17755; UvrA_DNA-bind; 1.
DR Pfam; PF17760; UvrA_inter; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR00630; uvra; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA damage; DNA excision; DNA repair; DNA-binding;
KW Excision nuclease; Metal-binding; Nucleotide-binding; Reference proteome;
KW Repeat; SOS response; Zinc; Zinc-finger.
FT CHAIN 1..945
FT /note="UvrABC system protein A"
FT /id="PRO_0000093078"
FT DOMAIN 310..587
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT DOMAIN 607..937
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 254..281
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 740..766
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 31..38
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 640..647
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
SQ SEQUENCE 945 AA; 104489 MW; 30074C2D4C2889C1 CRC64;
MDKILIRGAR THNLKNVDLT LPRDKLIVIT GLSGSGKSSL AFDTLYAEGQ RRYVESLSAY
ARQFLSMMEK PDVDTIEGLS PAISIEQKST SHNPRSTVGT ITEIYDYLRL LYARVGTPRC
PDHDIPLEAQ TVSQMVDQVL ALPEGSKLML LAPVIRERKG EHLAVFDEMR AQGFVRARVD
GKLYELDEVP KLDKQKKHSI DVVVDRFKVR ADLQQRLAES FETALSLADG IALVAPMDED
EDVEEIIFSA RFACPVCGHS ISELEPKLFS FNNPAGACPT CDGLGVKQFF DARRVVNGEL
TLAEGAIRGW DRRNVYYFQM LGSLAQHYGF SLEEPFDELG AEHQKVVLYG SGRENVDFRY
LNDRGDIVKR SHPFEGILPN LERRYRETES ATVREELAKF LSTQPCPDCH GTRLRREARH
VWVGDRTLPA ITAMPVGEAC EYAAGLSLTG RRGEIAAKIL KEIRDRLQFL VNVGLDYLTL
DRSADTLSGG EAQRIRLASQ IGAGLVGVMY ILDEPSIGLH QRDNERLLGT LTHLRNLGNT
VIVVEHDEDA IRLADYVVDI GPGAGVHGGQ VVAEGTPDQV MNHPDSLTGK YLSGRKKIAV
PAKRTPRDKK KLLKLKGARG NNLQNVNLEI PVGLFTCITG VSGSGKSTLI NNTLFPITAT
ALNGATTLEV APYDSFDGLQ HLDKVVDIDQ SPIGRTPRSN PATYTGLFTP IRELFSGVPE
ARSRGYGPGR FSFNVKGGRC EACQGDGVIK VEMHFLPDIY VPCDVCKGKR YNRETLEIRY
KGKSIHEVLE MTIEEAREFF DAVPALARKL QTLMDVGLSY IKLGQSATTL SGGEAQRVKL
SRELSKRDTG KTLYILDEPT TGLHFADIQQ LLDVLHRLRD HGNTVVVIEH NLDVIKTADW
LVDLGPEGGS KGGQIIANGT PEQVAEMPQS HTGHFLKPLL ERDRA
