UVRA_RHIME
ID UVRA_RHIME Reviewed; 973 AA.
AC P56899;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=UvrABC system protein A {ECO:0000255|HAMAP-Rule:MF_00205};
DE Short=UvrA protein {ECO:0000255|HAMAP-Rule:MF_00205};
DE AltName: Full=Excinuclease ABC subunit A {ECO:0000255|HAMAP-Rule:MF_00205};
GN Name=uvrA {ECO:0000255|HAMAP-Rule:MF_00205}; OrderedLocusNames=R01557;
GN ORFNames=SMc01235;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-140.
RC STRAIN=2021;
RX PubMed=10503543; DOI=10.1007/s004380051066;
RA Tapias A., Barbe J.;
RT "Regulation of divergent transcription from the uvrA-ssb promoters in
RT Sinorhizobium meliloti.";
RL Mol. Gen. Genet. 262:121-130(1999).
CC -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein.
CC A damage recognition complex composed of 2 UvrA and 2 UvrB subunits
CC scans DNA for abnormalities. When the presence of a lesion has been
CC verified by UvrB, the UvrA molecules dissociate. {ECO:0000255|HAMAP-
CC Rule:MF_00205}.
CC -!- SUBUNIT: Forms a heterotetramer with UvrB during the search for
CC lesions. {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. UvrA family.
CC {ECO:0000255|HAMAP-Rule:MF_00205}.
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DR EMBL; AL591688; CAC46136.1; -; Genomic_DNA.
DR EMBL; AF125162; AAF03210.1; -; Genomic_DNA.
DR RefSeq; NP_385663.1; NC_003047.1.
DR RefSeq; WP_003529545.1; NC_003047.1.
DR AlphaFoldDB; P56899; -.
DR SMR; P56899; -.
DR STRING; 266834.SMc01235; -.
DR EnsemblBacteria; CAC46136; CAC46136; SMc01235.
DR GeneID; 61603019; -.
DR KEGG; sme:SMc01235; -.
DR PATRIC; fig|266834.11.peg.2982; -.
DR eggNOG; COG0178; Bacteria.
DR HOGENOM; CLU_001370_0_2_5; -.
DR OMA; PFEGIIP; -.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00205; UvrA; 1.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004602; UvrA.
DR InterPro; IPR041552; UvrA_DNA-bd.
DR InterPro; IPR041102; UvrA_inter.
DR Pfam; PF17755; UvrA_DNA-bind; 1.
DR Pfam; PF17760; UvrA_inter; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR00630; uvra; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA damage; DNA excision; DNA repair; DNA-binding;
KW Excision nuclease; Metal-binding; Nucleotide-binding; Reference proteome;
KW Repeat; SOS response; Zinc; Zinc-finger.
FT CHAIN 1..973
FT /note="UvrABC system protein A"
FT /id="PRO_0000093082"
FT DOMAIN 331..609
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT DOMAIN 629..958
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 761..787
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 34..41
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 662..669
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT CONFLICT 19
FT /note="G -> A (in Ref. 3; AAF03210)"
FT /evidence="ECO:0000305"
FT CONFLICT 67
FT /note="F -> S (in Ref. 3; AAF03210)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 973 AA; 107192 MW; 3E1A8B14527A47FE CRC64;
MSELKTISIR GAREHNLKGI DLDLPRNKLI VMTGLSGSGK SSLAFDTIYA EGQRRYVESL
SAYARQFLEM MQKPDVDQID GLSPAISIEQ KTTSRNPRST VGTVTEIYDY LRLLFARVGV
PYSPATGLPI ESQTVSQMVD RVLEFGEGTR LYMLAPLVRG RKGEYRKELA ELMKKGFQRV
KVDGQFYEIA DVPALDKKYK HDIDVVVDRV VVRPDIGTRL ADSIETCLTL ADGLAIAEFA
DRPLPPEETS AGGSANKSLN ETHERVLFSE KFACPVSGFT IPEIEPRLFS FNNPFGACTT
CDGLGSQQKI DEALIVPEPN RTLRDGAIAP WAKSTSPYYN QTLEALGTVF GFKLGSRWSE
LSEEAQEAIL HGTKDKITFH YQDGARSYNT TKTFEGIVPN LERRWKETDS AWAREEIERY
MSAAPCPACA GYRLKPEALA VKIHALHIGE VSEMSIRAAR DWFEVLPEHL STKQNEIAVR
ILKEIRERLR FLNDVGLEYL SLSRNSGTLS GGESQRIRLA SQIGSGLTGV LYVLDEPSIG
LHQRDNARLL DTLRHLRDIG NTVIVVEHDE DAILTADYVV DIGPAAGIHG GEVIAEGTPS
DIMSNPKSLT GKYLSGELSV AVPGERRKPK KKKEVTVVGA RANNLKNVTA SIPLGVFTAV
TGVSGGGKST FLIETLYKAA ARRVMGAREN PAEHDRIDGF EHIDKVIDID QSPIGRTPRS
NPATYTGAFT PIRDWFAGLP EAKARGYQPG RFSFNVKGGR CEACQGDGVI KIEMHFLPDV
YVTCDVCHGK RYNRETLDVH FKGKSIADVL DMTVEEGVEF FAAVPAVRDK LVTLNQVGLG
YIKIGQQANT LSGGEAQRVK LAKELSKRST GRTLYILDEP TTGLHFHDVA KLLEVLHELV
NQGNSVVVIE HNLEVIKTAD WIIDFGPEGG DGGGEVIAQG TPEEVVKEPR SYTGQFLKEL
LERRPVKKVV AAE
