UVRA_STRP1
ID UVRA_STRP1 Reviewed; 942 AA.
AC Q99Y84; Q48WV7;
DT 08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=UvrABC system protein A {ECO:0000255|HAMAP-Rule:MF_00205};
DE Short=UvrA protein {ECO:0000255|HAMAP-Rule:MF_00205};
DE AltName: Full=Excinuclease ABC subunit A {ECO:0000255|HAMAP-Rule:MF_00205};
GN Name=uvrA {ECO:0000255|HAMAP-Rule:MF_00205};
GN OrderedLocusNames=SPy_1825, M5005_Spy1550;
OS Streptococcus pyogenes serotype M1.
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=301447;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700294 / SF370 / Serotype M1;
RX PubMed=11296296; DOI=10.1073/pnas.071559398;
RA Ferretti J.J., McShan W.M., Ajdic D.J., Savic D.J., Savic G., Lyon K.,
RA Primeaux C., Sezate S., Suvorov A.N., Kenton S., Lai H.S., Lin S.P.,
RA Qian Y., Jia H.G., Najar F.Z., Ren Q., Zhu H., Song L., White J., Yuan X.,
RA Clifton S.W., Roe B.A., McLaughlin R.E.;
RT "Complete genome sequence of an M1 strain of Streptococcus pyogenes.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:4658-4663(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-947 / MGAS5005 / Serotype M1;
RX PubMed=16088826; DOI=10.1086/432514;
RA Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K.,
RA Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P.,
RA Musser J.M.;
RT "Evolutionary origin and emergence of a highly successful clone of serotype
RT M1 group A Streptococcus involved multiple horizontal gene transfer
RT events.";
RL J. Infect. Dis. 192:771-782(2005).
CC -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein.
CC A damage recognition complex composed of 2 UvrA and 2 UvrB subunits
CC scans DNA for abnormalities. When the presence of a lesion has been
CC verified by UvrB, the UvrA molecules dissociate. {ECO:0000255|HAMAP-
CC Rule:MF_00205}.
CC -!- SUBUNIT: Forms a heterotetramer with UvrB during the search for
CC lesions. {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. UvrA family.
CC {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAZ52168.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE004092; AAK34548.1; -; Genomic_DNA.
DR EMBL; CP000017; AAZ52168.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_269827.1; NC_002737.2.
DR AlphaFoldDB; Q99Y84; -.
DR SMR; Q99Y84; -.
DR STRING; 1314.HKU360_01600; -.
DR PaxDb; Q99Y84; -.
DR EnsemblBacteria; AAK34548; AAK34548; SPy_1825.
DR KEGG; spy:SPy_1825; -.
DR KEGG; spz:M5005_Spy1550; -.
DR PATRIC; fig|160490.10.peg.1585; -.
DR HOGENOM; CLU_001370_0_2_9; -.
DR OMA; PFEGIIP; -.
DR Proteomes; UP000000750; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProt.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00205; UvrA; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004602; UvrA.
DR InterPro; IPR041552; UvrA_DNA-bd.
DR InterPro; IPR041102; UvrA_inter.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF17755; UvrA_DNA-bind; 1.
DR Pfam; PF17760; UvrA_inter; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR00630; uvra; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA damage; DNA excision; DNA repair; DNA-binding;
KW Excision nuclease; Metal-binding; Nucleotide-binding; Reference proteome;
KW Repeat; SOS response; Zinc; Zinc-finger.
FT CHAIN 1..942
FT /note="UvrABC system protein A"
FT /id="PRO_0000093102"
FT DOMAIN 308..589
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT DOMAIN 609..937
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 251..278
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 740..766
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 32..39
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 641..648
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT CONFLICT 428
FT /note="T -> P (in Ref. 2; AAZ52168)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 942 AA; 103970 MW; FCA4B28CDCE76C90 CRC64;
MQNKIIIHGA RAHNLKNIDV EIPRDKLVVV TGLSGSGKSS LAFDTIYAEG QRRYVESLSA
YARQFLGNME KPDVDSIDGL SPAISIDQKT TSKNPRSTVG TVTEINDYLR LLYARVGTPY
CINGHGAITA SSAEQIVEQV LALPERTRMQ ILAPVVRRKK GQHKTVFEKI QKDGYVRVRV
DGDIFDVTEV PELSKSKMHN IEVVIDRLVN KDGIRSRLFD SVEAALRLGD GYLMIDTMDG
NELLFSEHYS CPVCGFTVPE LEPRLFSFNA PFGSCPTCDG LGIKLEVDLD LVVPDPSKSL
REGALAPWNP ISSNYYPTML EQAMASFGVD MDTPFEALTE EERDLVLYGS GDREFHFHYV
NDFGGERNID IPFEGVVTNV NRRYHETNSD YTRNVMRGYM NELTCATCHG YRLNDQALCV
HVGGEEGTHI GQISELSIAD HLQLLEELEL TENESTIAKP IVKEIHDRLT FLNNVGLNYL
TLSRAAGTLS GGESQRIRLA TQIGSNLSGV LYILDEPSIG LHQRDNDRLI ESLKKMRDLG
NTLIVVEHDE DTMMQADWLI DVGPGAGEFG GEITASGTPK QVAKNKKSIT GQYLSGKKFI
PVPLERRSGN GRFIEIKGAA QNNLQSLDVR FPLGKFIAVT GVSGSGKSTL VNSILKKAVA
QKLNRNADKP GKYHSISGIE HIERLIDIDQ SPIGRTPRSN PATYTGVFDD IRDLFAQTNE
AKIRGYKKGR FSFNVKGGRC EACSGDGIIK IEMHFLPDVY VPCEVCHGRR YNSETLEVHY
KGKNIAEVLD MTVDDALVFF SAIPKIARKI QTIKDVGLGY VTLGQPATTL SGGEAQRMKL
ASELHKRSTG KSLYILDEPT TGLHTDDIAR LLKVLERFVD DGNTVLVIEH NLDVIKSADH
IIDLGPEGGD GGGQIVATGT PEEVAQVKES YTGHYLKVKL QQ
