UVRA_THEMA
ID UVRA_THEMA Reviewed; 916 AA.
AC Q9WYV0;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=UvrABC system protein A {ECO:0000255|HAMAP-Rule:MF_00205};
DE Short=UvrA protein {ECO:0000255|HAMAP-Rule:MF_00205};
DE AltName: Full=Excinuclease ABC subunit A {ECO:0000255|HAMAP-Rule:MF_00205};
GN Name=uvrA {ECO:0000255|HAMAP-Rule:MF_00205}; OrderedLocusNames=TM_0480;
OS Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS / MSB8).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=243274;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=10360571; DOI=10.1038/20601;
RA Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA Smith H.O., Venter J.C., Fraser C.M.;
RT "Evidence for lateral gene transfer between Archaea and Bacteria from
RT genome sequence of Thermotoga maritima.";
RL Nature 399:323-329(1999).
CC -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein.
CC A damage recognition complex composed of 2 UvrA and 2 UvrB subunits
CC scans DNA for abnormalities. When the presence of a lesion has been
CC verified by UvrB, the UvrA molecules dissociate. {ECO:0000255|HAMAP-
CC Rule:MF_00205}.
CC -!- SUBUNIT: Forms a heterotetramer with UvrB during the search for
CC lesions. {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. UvrA family.
CC {ECO:0000255|HAMAP-Rule:MF_00205}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE000512; AAD35564.1; -; Genomic_DNA.
DR PIR; H72372; H72372.
DR RefSeq; NP_228290.1; NC_000853.1.
DR RefSeq; WP_004081486.1; NZ_CP011107.1.
DR PDB; 3PIH; X-ray; 2.90 A; A=1-916.
DR PDB; 6N9L; X-ray; 2.01 A; A=2-916.
DR PDBsum; 3PIH; -.
DR PDBsum; 6N9L; -.
DR AlphaFoldDB; Q9WYV0; -.
DR SMR; Q9WYV0; -.
DR DIP; DIP-59073N; -.
DR STRING; 243274.THEMA_02285; -.
DR EnsemblBacteria; AAD35564; AAD35564; TM_0480.
DR KEGG; tma:TM0480; -.
DR eggNOG; COG0178; Bacteria.
DR InParanoid; Q9WYV0; -.
DR OMA; PFEGIIP; -.
DR OrthoDB; 152379at2; -.
DR Proteomes; UP000008183; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00205; UvrA; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004602; UvrA.
DR InterPro; IPR041552; UvrA_DNA-bd.
DR InterPro; IPR041102; UvrA_inter.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF17755; UvrA_DNA-bind; 1.
DR Pfam; PF17760; UvrA_inter; 1.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR00630; uvra; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cytoplasm; DNA damage; DNA excision; DNA repair;
KW DNA-binding; Excision nuclease; Metal-binding; Nucleotide-binding;
KW Reference proteome; Repeat; SOS response; Zinc; Zinc-finger.
FT CHAIN 1..916
FT /note="UvrABC system protein A"
FT /id="PRO_0000093107"
FT DOMAIN 308..566
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT DOMAIN 586..913
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 252..279
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 716..742
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 31..38
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 617..624
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT STRAND 3..9
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 16..30
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 37..41
FT /evidence="ECO:0007829|PDB:6N9L"
FT TURN 42..44
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 45..54
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 74..77
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 82..89
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 98..101
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 104..113
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 117..119
FT /evidence="ECO:0007829|PDB:3PIH"
FT TURN 121..123
FT /evidence="ECO:0007829|PDB:3PIH"
FT HELIX 132..142
FT /evidence="ECO:0007829|PDB:3PIH"
FT STRAND 148..152
FT /evidence="ECO:0007829|PDB:3PIH"
FT STRAND 206..209
FT /evidence="ECO:0007829|PDB:3PIH"
FT HELIX 212..214
FT /evidence="ECO:0007829|PDB:3PIH"
FT HELIX 215..229
FT /evidence="ECO:0007829|PDB:3PIH"
FT STRAND 231..237
FT /evidence="ECO:0007829|PDB:3PIH"
FT TURN 238..240
FT /evidence="ECO:0007829|PDB:3PIH"
FT STRAND 243..249
FT /evidence="ECO:0007829|PDB:3PIH"
FT TURN 253..255
FT /evidence="ECO:0007829|PDB:3PIH"
FT HELIX 264..267
FT /evidence="ECO:0007829|PDB:3PIH"
FT TURN 272..274
FT /evidence="ECO:0007829|PDB:3PIH"
FT TURN 277..281
FT /evidence="ECO:0007829|PDB:3PIH"
FT STRAND 282..288
FT /evidence="ECO:0007829|PDB:3PIH"
FT HELIX 306..308
FT /evidence="ECO:0007829|PDB:3PIH"
FT HELIX 312..322
FT /evidence="ECO:0007829|PDB:3PIH"
FT TURN 323..325
FT /evidence="ECO:0007829|PDB:3PIH"
FT HELIX 342..346
FT /evidence="ECO:0007829|PDB:3PIH"
FT HELIX 354..363
FT /evidence="ECO:0007829|PDB:3PIH"
FT HELIX 371..376
FT /evidence="ECO:0007829|PDB:3PIH"
FT STRAND 379..383
FT /evidence="ECO:0007829|PDB:3PIH"
FT TURN 385..387
FT /evidence="ECO:0007829|PDB:3PIH"
FT HELIX 396..398
FT /evidence="ECO:0007829|PDB:3PIH"
FT STRAND 399..401
FT /evidence="ECO:0007829|PDB:3PIH"
FT HELIX 406..410
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 414..422
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 430..450
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 462..464
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 467..481
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 487..491
FT /evidence="ECO:0007829|PDB:6N9L"
FT TURN 493..496
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 499..501
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 502..514
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 518..522
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 526..530
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 533..542
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 543..545
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 548..553
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 555..560
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 566..571
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 573..575
FT /evidence="ECO:0007829|PDB:3PIH"
FT STRAND 587..595
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 602..616
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 623..627
FT /evidence="ECO:0007829|PDB:6N9L"
FT TURN 628..630
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 631..639
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 650..653
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 655..657
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 659..663
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 677..680
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 683..692
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 695..700
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 704..707
FT /evidence="ECO:0007829|PDB:6N9L"
FT TURN 712..714
FT /evidence="ECO:0007829|PDB:6N9L"
FT TURN 717..721
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 722..726
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 730..732
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 736..738
FT /evidence="ECO:0007829|PDB:6N9L"
FT TURN 740..744
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 749..752
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 761..765
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 766..768
FT /evidence="ECO:0007829|PDB:3PIH"
FT HELIX 769..775
FT /evidence="ECO:0007829|PDB:6N9L"
FT TURN 776..778
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 780..791
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 794..796
FT /evidence="ECO:0007829|PDB:3PIH"
FT HELIX 803..805
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 808..821
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 827..833
FT /evidence="ECO:0007829|PDB:6N9L"
FT TURN 835..838
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 841..856
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 860..864
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 868..871
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 875..884
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 885..887
FT /evidence="ECO:0007829|PDB:6N9L"
FT STRAND 890..895
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 897..901
FT /evidence="ECO:0007829|PDB:6N9L"
FT HELIX 907..912
FT /evidence="ECO:0007829|PDB:6N9L"
FT TURN 913..915
FT /evidence="ECO:0007829|PDB:6N9L"
SQ SEQUENCE 916 AA; 102967 MW; 54FBAA620783791A CRC64;
MNEIVVKGAR VHNLKNITVR IPKNRLVVIT GVSGSGKSSL AMDTIYAEGQ RRYLESLSTY
ARQFLGNLKK PDVDEIEGLS PAIAIDQKTV SHNPRSTVGT VTEIYDYLRV LYARIGKAHC
PECGRPLEKK SIDEILQDLF NSFKEGSRIY ILAPVATEKK GTFKKEIEEF ISKGFARIEI
DGEIYRLEEV PELDKNKRHT VKLVVDRLIL ETRNEHRILD SLELAMREGK GFVEIRNVDT
GESKIFSENL MCPVCGIGFP EITPKLFSFN SPYGACPNCH GLGFTFEVDP SLVIDEEKSV
LEGAIIPYRW DRRLSRWVAR EIEKRGVSPH LPFKDLPEDV KEFILYGDDR FEGVVPKVQR
WHRETESPEM KEWLEKNFIV QRTCSVCGGR RLNREALSVK INGLNIHEFT ELSISEELEF
LKNLNLTERE REIVGELLKE IEKRLEFLVD VGLEYLTLSR SATTLSGGES QRIRLATQIG
SGLTGVIYVL DEPTIGLHPR DTERLIKTLK KLRDLGNTVI VVEHDEEVIR NADHIIDIGP
GGGTNGGRVV FQGTVDELLK NPDSSLTGEY LSGKRKITVN KTRRLPYASL KIKGVRHNNL
KNIDVEIPLG VFVCVTGVSG SGKSSLVMET LYPALMNLLH KTKLPAGEFD SIEGHENIDK
MIAIDQSPIG RTPRSNPATY TKVFDEIRSL FAMTPAAKAR GYNKSRFSFN LKGGRCEACQ
GQGYVKIEML FLPDVYVECD VCKGKRYNRE TLEITYKGKN ISDILDMTVD EALEFFKNIP
SIKRTLQVLH DVGLGYVKLG QPATTLSGGE AQRIKLASEL RKRDTGRTLY ILDEPTVGLH
FEDVRKLVEV LHRLVDRGNT VIVIEHNLDV IKNADHIIDL GPEGGKEGGY IVATGTPEEI
AKNPHSYTGR FLKNVL
