UVRA_THET8
ID UVRA_THET8 Reviewed; 952 AA.
AC Q56242; Q5SID4;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 29-MAR-2005, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=UvrABC system protein A {ECO:0000255|HAMAP-Rule:MF_00205};
DE Short=UvrA protein {ECO:0000255|HAMAP-Rule:MF_00205};
DE AltName: Full=Excinuclease ABC subunit A {ECO:0000255|HAMAP-Rule:MF_00205};
GN Name=uvrA {ECO:0000255|HAMAP-Rule:MF_00205}; OrderedLocusNames=TTHA1440;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8675016; DOI=10.1016/0378-1119(96)00052-2;
RA Yamamoto N., Kato R., Kuramitsu S.;
RT "Cloning, sequencing and expression of the uvrA gene from an extremely
RT thermophilic bacterium, Thermus thermophilus HB8.";
RL Gene 171:103-106(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The UvrABC repair system catalyzes the recognition and
CC processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein.
CC A damage recognition complex composed of 2 UvrA and 2 UvrB subunits
CC scans DNA for abnormalities. When the presence of a lesion has been
CC verified by UvrB, the UvrA molecules dissociate. {ECO:0000255|HAMAP-
CC Rule:MF_00205}.
CC -!- SUBUNIT: Forms a heterotetramer with UvrB during the search for
CC lesions. {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00205}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. UvrA family.
CC {ECO:0000255|HAMAP-Rule:MF_00205}.
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DR EMBL; D49911; BAA08652.1; -; Genomic_DNA.
DR EMBL; AP008226; BAD71263.1; -; Genomic_DNA.
DR RefSeq; WP_011228683.1; NC_006461.1.
DR RefSeq; YP_144706.1; NC_006461.1.
DR AlphaFoldDB; Q56242; -.
DR SMR; Q56242; -.
DR STRING; 300852.55772822; -.
DR EnsemblBacteria; BAD71263; BAD71263; BAD71263.
DR GeneID; 3169496; -.
DR KEGG; ttj:TTHA1440; -.
DR PATRIC; fig|300852.9.peg.1414; -.
DR eggNOG; COG0178; Bacteria.
DR HOGENOM; CLU_001370_0_2_0; -.
DR OMA; PFEGIIP; -.
DR PhylomeDB; Q56242; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009380; C:excinuclease repair complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009381; F:excinuclease ABC activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006289; P:nucleotide-excision repair; IEA:UniProtKB-UniRule.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00205; UvrA; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR004602; UvrA.
DR InterPro; IPR041552; UvrA_DNA-bd.
DR InterPro; IPR041102; UvrA_inter.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF17755; UvrA_DNA-bind; 1.
DR Pfam; PF17760; UvrA_inter; 1.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR00630; uvra; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 2.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; DNA damage; DNA excision; DNA repair; DNA-binding;
KW Excision nuclease; Metal-binding; Nucleotide-binding; Reference proteome;
KW Repeat; SOS response; Zinc; Zinc-finger.
FT CHAIN 1..952
FT /note="UvrABC system protein A"
FT /id="PRO_0000093108"
FT DOMAIN 309..591
FT /note="ABC transporter 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT DOMAIN 611..938
FT /note="ABC transporter 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 253..280
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT ZN_FING 742..768
FT /note="C4-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 31..38
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT BINDING 643..650
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00205"
FT CONFLICT 837
FT /note="A -> S (in Ref. 1; BAA08652)"
FT /evidence="ECO:0000305"
FT CONFLICT 844
FT /note="T -> K (in Ref. 1; BAA08652)"
FT /evidence="ECO:0000305"
FT CONFLICT 847..849
FT /note="GRK -> RPQ (in Ref. 1; BAA08652)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 952 AA; 105150 MW; EF88FAA636126EDA CRC64;
MDRIVIRGAR EHNLKNISLE LPRGKFIVIT GVSGSGKSTL AFDTIYAEGQ RRYVESLSSY
ARQFLGVMDK PEVESIEGLS PAISIDQKTT SHNPRSTVGT VTEIHDYLRL LFARVGQAFC
PECGRPIEKQ SASEITDRLL KRPPGTRAIL MAPLVRGRKG EYRKLFQQLL KEGYARVRVD
GVIYLLEEAQ GLSLEKYEKH DIDLVIDRVV LKEEERPRIA EAVELALLRG EGLLRVLYPD
TGEEELFSEK FACPEHGSVL EELEPRIFSF NSPYGACPAC SGLGYRQEFD PELVVNPELS
LAEGAILPWS RGRDTGRSYL WDRLRALAEH LGFDLKTPFK DLPEEAKRAV LYGLPEPFEV
VFRRGGKETF RVEVRYEGVI PWLEKRYQES DSEGVREALE GFMSLRPCPA CGGTRYKREV
LSVKVAGRNI AEVSALPVRE ALAFFQGLEK TLPPFQAQIA RPILREIVER LGFLVDVGLD
YLTLDRAANT LSGGEAQRIR LATQVGSGLT GVLYVLDEPS IGLHPRDNQR LIRTLKRLRD
LGNTLIVVEH DEETMRAADW IVDMGPGAGI HGGEVVAQGT LEDILKSPQS LTGAYLRGEK
RIPVPKERRK GNGKWLVLKG ARAHNLKNVT LRIPLGRFVA ITGPSGSGKS TLVHDVLYAA
LAQRLMRAKT TPGPYEALEG VEHLDKVIEI DQSPIGRTPR SNPATYTGVF DEIRDLFAKT
PEARKRGYGP GRFSFNVKGG RCEACGGDGT VKIEMLFLPD LYVPCEVCKG KRYNKETLEV
KLRGKSIADV LDMTVEEALD FFQNVPSIAR KLQLMVDVGL GYMKLGQPSP TLSGGEAQRI
KLATELGRKA TGRTLYILDE PTTGLHFDDV AKLLSVLHRL VDAGNTVVVI EHNLDVVKTA
DWVIDLGPEG GDRGGEIVAE GTPEEVALTG SPTGAFLARI PEIAARIGVA AD
