ID   CAEB_MYCTU              Reviewed;         520 AA.
AC   P9WHR5; L0TBV4; P65821; Q10508;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Carboxylesterase B {ECO:0000250|UniProtKB:P9WHR4};
DE            EC=3.1.1.- {ECO:0000250|UniProtKB:P9WHR4};
DE   Flags: Precursor;
GN   Name=caeB; OrderedLocusNames=Rv2223c; ORFNames=MTCY427.04c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase S33 family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP45001.1; -; Genomic_DNA.
DR   PIR; C70776; C70776.
DR   RefSeq; NP_216739.1; NC_000962.3.
DR   RefSeq; WP_003411484.1; NZ_NVQJ01000008.1.
DR   AlphaFoldDB; P9WHR5; -.
DR   SMR; P9WHR5; -.
DR   STRING; 83332.Rv2223c; -.
DR   ESTHER; myctu-ym23; AlphaBeta_hydrolase.
DR   PaxDb; P9WHR5; -.
DR   DNASU; 888093; -.
DR   GeneID; 888093; -.
DR   KEGG; mtu:Rv2223c; -.
DR   PATRIC; fig|83332.111.peg.2472; -.
DR   TubercuList; Rv2223c; -.
DR   eggNOG; COG0596; Bacteria.
DR   OMA; NQMAGFQ; -.
DR   PhylomeDB; P9WHR5; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR013595; Pept_S33_TAP-like_C.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   Pfam; PF08386; Abhydrolase_4; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..34
FT                   /evidence="ECO:0000255"
FT   CHAIN           35..520
FT                   /note="Carboxylesterase B"
FT                   /id="PRO_0000027327"
FT   DOMAIN          105..403
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        232
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:P9WHR3"
FT   ACT_SITE        461
FT                   /evidence="ECO:0000250|UniProtKB:P9WHR3"
FT   ACT_SITE        488
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P9WHR3"
SQ   SEQUENCE   520 AA;  55078 MW;  7D05F1533A4C6B91 CRC64;
     MAAMWRRRPL SSALLSFGLL LGGLPLAAPP LAGATEEPGA GQTPGAPVVA PQQSWNSCRE
     FIADTSEIRT ARCATVSVPV DYDQPGGTQA KLAVIRVPAT GQRFGALLVN PGGPGASAVD
     MVAAMAPAIA DTDILRHFDL VGFDPRGVGH STPALRCRTD AEFDAYRRDP MADYSPAGVT
     HVEQVYRQLA QDCVDRMGFS FLANIGTASV ARDMDMVRQA LGDDQINYLG YSYGTELGTA
     YLERFGTHVR AMVLDGAIDP AVSPIEESIS QMAGFQTAFN DYAADCARSP ACPLGTDSAQ
     WVNRYHALVD PLVQKPGKTS DPRGLSYADA TTGTINALYS PQRWKYLTSG LLGLQRGSDA
     GDLLVLADDY DGRDADGHYS NDQDAFNAVR CVDAPTPADP AAWVAADQRI RQVAPFLSYG
     QFTGSAPRDL CALWPVPATS TPHPAAPAGA GKVVVVSTTH DPATPYQSGV DLARQLGAPL
     ITFDGTQHTA VFDGNQCVDS AVMHYFLDGT LPPTSLRCAP