CAF1A_CHICK
ID CAF1A_CHICK Reviewed; 937 AA.
AC Q5R1T0; Q5ZK85;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 04-JAN-2005, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Chromatin assembly factor 1 subunit A;
DE Short=CAF-1 subunit A;
DE AltName: Full=Chromatin assembly factor I p150 subunit;
DE Short=CAF-I 150 kDa subunit;
DE Short=CAF-I p150;
GN Name=CHAF1A; Synonyms=CAIP150; ORFNames=RCJMB04_12f19;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, AND INTERACTION
RP WITH CHAF1B; PCNA AND RBBP4.
RC TISSUE=B-cell;
RX PubMed=17065558; DOI=10.1091/mbc.e06-05-0426;
RA Takami Y., Ono T., Fukagawa T., Shibahara K., Nakayama T.;
RT "Essential role of chromatin assembly factor-1-mediated rapid nucleosome
RT assembly for DNA replication and cell division in vertebrate cells.";
RL Mol. Biol. Cell 18:129-141(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=CB; TISSUE=Bursa of Fabricius;
RX PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA Hayashizaki Y., Buerstedde J.-M.;
RT "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT function analysis.";
RL Genome Biol. 6:R6.1-R6.9(2005).
CC -!- FUNCTION: Complex that is thought to mediate chromatin assembly in DNA
CC replication and DNA repair. Assembles histone octamers onto replicating
CC DNA in vitro. CAF-1 performs the first step of the nucleosome assembly
CC process, bringing newly synthesized histones H3 and H4 to replicating
CC DNA; histones H2A/H2B can bind to this chromatin precursor subsequent
CC to DNA replication to complete the histone octamer.
CC {ECO:0000269|PubMed:17065558}.
CC -!- SUBUNIT: Subunit of the CAF-1 complex that contains RBBP4, CHAF1B and
CC CHAF1A. Interacts with CHAF1B, PCNA and RBBP4 (PubMed:17065558).
CC {ECO:0000269|PubMed:17065558}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q13111}. Note=DNA
CC replication foci. {ECO:0000250|UniProtKB:Q13111}.
CC -!- DOMAIN: Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is
CC required for interaction with chromoshadow domains.
CC -!- DISRUPTION PHENOTYPE: Cells lacking CHAF1A show delayed S-phase
CC progression with retarded DNA synthesis and defects in a rapid
CC nucleosome formation of newly replicated DNA.
CC {ECO:0000269|PubMed:17065558}.
CC -!- SIMILARITY: Belongs to the CHAF1A family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAG31858.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAG31858.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB195692; BAD72952.1; -; mRNA.
DR EMBL; AJ720199; CAG31858.1; ALT_SEQ; mRNA.
DR RefSeq; NP_001073220.2; NM_001079752.2.
DR AlphaFoldDB; Q5R1T0; -.
DR SMR; Q5R1T0; -.
DR BioGRID; 690937; 3.
DR STRING; 9031.ENSGALP00000001693; -.
DR PaxDb; Q5R1T0; -.
DR GeneID; 770217; -.
DR KEGG; gga:770217; -.
DR CTD; 10036; -.
DR VEuPathDB; HostDB:geneid_770217; -.
DR eggNOG; KOG4364; Eukaryota.
DR InParanoid; Q5R1T0; -.
DR OrthoDB; 1362011at2759; -.
DR PhylomeDB; Q5R1T0; -.
DR PRO; PR:Q5R1T0; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0033186; C:CAF-1 complex; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0031497; P:chromatin assembly; ISS:UniProtKB.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0006334; P:nucleosome assembly; IBA:GO_Central.
DR InterPro; IPR029105; CAF1-p150_C2.
DR InterPro; IPR029091; CAF1_p150_N.
DR InterPro; IPR022043; CAF1A.
DR Pfam; PF15539; CAF1-p150_C2; 1.
DR Pfam; PF15557; CAF1-p150_N; 1.
DR Pfam; PF12253; CAF1A; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Chaperone; DNA damage; DNA repair; DNA replication; Nucleus;
KW Reference proteome.
FT CHAIN 1..937
FT /note="Chromatin assembly factor 1 subunit A"
FT /id="PRO_0000373882"
FT REGION 21..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 250..386
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 574..614
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 753..778
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 831..851
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 910..937
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 213..226
FT /note="PxVxL motif"
FT /evidence="ECO:0000250"
FT COMPBIAS 27..45
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 50..69
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..296
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 297..386
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 574..608
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 753..770
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 62
FT /note="L -> S (in Ref. 2; CAG31858)"
FT /evidence="ECO:0000305"
FT CONFLICT 97
FT /note="Q -> R (in Ref. 2; CAG31858)"
FT /evidence="ECO:0000305"
FT CONFLICT 148
FT /note="M -> T (in Ref. 2; CAG31858)"
FT /evidence="ECO:0000305"
FT CONFLICT 247
FT /note="V -> I (in Ref. 2; CAG31858)"
FT /evidence="ECO:0000305"
FT CONFLICT 611
FT /note="I -> V (in Ref. 2; CAG31858)"
FT /evidence="ECO:0000305"
FT CONFLICT 842
FT /note="V -> I (in Ref. 2; CAG31858)"
FT /evidence="ECO:0000305"
FT CONFLICT 856
FT /note="S -> C (in Ref. 2; CAG31858)"
FT /evidence="ECO:0000305"
FT CONFLICT 914
FT /note="T -> A (in Ref. 2; CAG31858)"
FT /evidence="ECO:0000305"
FT CONFLICT 919
FT /note="R -> S (in Ref. 2; CAG31858)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 937 AA; 105133 MW; CD4A599B43B506D0 CRC64;
MAAMECRDKA VVPPRRLVQA RLPFKRLNPV PKEKHDAEAE GKKGKCSKSG LGQSKDSSTD
TLHASTDNME NDCQLNSDVN FVPKLVNGKG PLDHFIQKST KDNTDEIIVI IDSVDESNQR
LSDDVDHVSF DSKASSAALT NGMLGKDMNK LSCLNSTQNS QTDDSMQTDV PCEAIANKGE
DLVDGIQSCS GLTERNNLEN TKVNQSELKD VIFEGKMPVV LLEDIMAAKS PQVTSLDGSV
TSENETVELS HEGDSVLTNS SLSSLSVSSP EAQPVAETKR NTSPLAVSTP VRKVSQKLHK
SSAEKEKLRL QRDQERADKL QKLQAEREEK GRLKEEAKAA KERAKEEAKK KKEEEKELKE
RERREKKEKE EKEKAEKLRV KEEKRKERQE ALEAKLEEKR KKEEEKRLKE EEKRINAQKA
EITRFFQKPK TPQAPKILAG SCGKFAPFEI KENMVLAPLC RIALYPDFLE QLDRLLRAQN
SEVSFLRDLK CRKPRKTGPT FVNSSTDTVN SDVVVVDNCK TDAVPERGKF GRMKLLQFCE
NHRPAYWGTW NKKTTMIRPR NPWSKDSKLL DYEVDSDEEW EEEEPGESLS HSEGDDEEEG
EDEDDDDGFF IPHGYLSEDE GVTEECDPEN QKVRQKLKAK EWDELMAKGK RLHVLQPVKI
GCIWESAQND SGTNADLKIL QQFTACILDS SVAEEEQQIQ KCSKKRAKDQ QILGQLLPLL
HGNVNGSKVI IQEFQECCRQ GLFSDVTAAT DCGDTSPVSP NTSRPQTPVG EDSGVPSKAR
LKRIISENSV YEKRPEYRMC WYVHSEVLKS FDQEHLPVPC QWNYITQVPS SGKEDGGSVP
GVAPVQSTPV SVKRKSTGSM CITKFMKRPR DAEQAEAMEM DGFQADTEED EDDDDCMIVD
VQPCKDSTLT VTETAPESRG TTAAHQDASM VSPSNTV
