CAF1A_YERPE
ID CAF1A_YERPE Reviewed; 833 AA.
AC P26949; O68773;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 16-NOV-2001, sequence version 2.
DT 25-MAY-2022, entry version 147.
DE RecName: Full=F1 capsule-anchoring protein;
DE Flags: Precursor;
GN Name=caf1A; OrderedLocusNames=YPMT1.83, Y1099, YP_pMT083;
OS Yersinia pestis.
OG Plasmid pMT1 (pMT-1), and Plasmid pFra.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=632;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC PLASMID=pFra;
RX PubMed=1551441; DOI=10.1016/0014-5793(92)80331-a;
RA Karlyshev A.V., Galyov E.E., Smirnov O.Y., Guzayev A.P., Abramov V.M.,
RA Zav'yalov V.P.;
RT "A new gene of the f1 operon of Y. pestis involved in the capsule
RT biogenesis.";
RL FEBS Lett. 297:77-80(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=KIM5 / Biovar Mediaevalis; PLASMID=pMT1 (pMT-1);
RX PubMed=9748454; DOI=10.1128/jb.180.19.5192-5202.1998;
RA Hu P., Elliott J., McCready P., Skowronski E., Garnes J., Kobayashi A.,
RA Brubaker R.R., Garcia E.;
RT "Structural organization of virulence-associated plasmids of Yersinia
RT pestis.";
RL J. Bacteriol. 180:5192-5202(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KIM10+ / Biovar Mediaevalis; PLASMID=pMT1 (pMT-1);
RX PubMed=9826348; DOI=10.1128/iai.66.12.5731-5742.1998;
RA Lindler L.E., Plano G.V., Burland V., Mayhew G.F., Blattner F.R.;
RT "Complete DNA sequence and detailed analysis of the Yersinia pestis KIM5
RT plasmid encoding murine toxin and capsular antigen.";
RL Infect. Immun. 66:5731-5742(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO-92 / Biovar Orientalis; PLASMID=pMT1 (pMT-1);
RX PubMed=11586360; DOI=10.1038/35097083;
RA Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA Stevens K., Whitehead S., Barrell B.G.;
RT "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL Nature 413:523-527(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=91001 / Biovar Mediaevalis; PLASMID=pMT1 (pMT-1);
RX PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA Wang J., Huang P., Yang R.;
RT "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT avirulent to humans.";
RL DNA Res. 11:179-197(2004).
CC -!- FUNCTION: A probable role in capsular biogenesis. It is likely that the
CC caf1A molecule binds F1 antigen subunits during the extracellular
CC secretion process.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the fimbrial export usher family. {ECO:0000305}.
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DR EMBL; X61996; CAA43968.1; -; Genomic_DNA.
DR EMBL; AF074611; AAC82757.1; -; Genomic_DNA.
DR EMBL; AF053947; AAC13219.1; -; Genomic_DNA.
DR EMBL; AL117211; CAB55265.1; -; Genomic_DNA.
DR EMBL; AE017045; AAS58715.1; -; Genomic_DNA.
DR PIR; S20387; S20387.
DR PIR; T14703; T14703.
DR RefSeq; NP_395429.1; NC_003134.1.
DR RefSeq; NP_857693.1; NC_004835.1.
DR RefSeq; WP_002211763.1; NZ_WUCL01000053.1.
DR RefSeq; YP_093953.1; NC_006323.1.
DR PDB; 2XET; X-ray; 1.60 A; A/B=745-833.
DR PDB; 3FCG; X-ray; 2.85 A; A/B=253-341.
DR PDB; 4B0E; X-ray; 2.00 A; A/B/C/D=23-158.
DR PDB; 4B0M; X-ray; 1.80 A; A=23-158.
DR PDBsum; 2XET; -.
DR PDBsum; 3FCG; -.
DR PDBsum; 4B0E; -.
DR PDBsum; 4B0M; -.
DR AlphaFoldDB; P26949; -.
DR SMR; P26949; -.
DR DIP; DIP-59979N; -.
DR IntAct; P26949; 1.
DR TCDB; 1.B.11.3.8; the outer membrane fimbrial usher porin (fup) family.
DR DNASU; 1149243; -.
DR EnsemblBacteria; AAC82757; AAC82757; AAC82757.
DR EnsemblBacteria; AAS58715; AAS58715; YP_pMT083.
DR GeneID; 57977635; -.
DR KEGG; ype:YPMT1.83; -.
DR KEGG; ypk:caf1A.pl; -.
DR KEGG; ypm:YP_pMT083; -.
DR PATRIC; fig|214092.21.peg.211; -.
DR HOGENOM; CLU_009120_3_1_6; -.
DR OMA; RMESYSA; -.
DR EvolutionaryTrace; P26949; -.
DR Proteomes; UP000000815; Plasmid pMT1 (pMT-1).
DR Proteomes; UP000001019; Plasmid pMT1.
DR Proteomes; UP000002490; Plasmid pMT-1.
DR GO; GO:0009279; C:cell outer membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015473; F:fimbrial usher porin activity; IBA:GO_Central.
DR GO; GO:0009297; P:pilus assembly; IBA:GO_Central.
DR Gene3D; 2.60.40.2070; -; 1.
DR Gene3D; 2.60.40.2610; -; 1.
DR Gene3D; 3.10.20.410; -; 1.
DR InterPro; IPR000015; Fimb_usher.
DR InterPro; IPR018030; Fimbrial_membr_usher_CS.
DR InterPro; IPR042186; FimD_plug_dom.
DR InterPro; IPR025949; PapC-like_C.
DR InterPro; IPR043142; PapC-like_C_sf.
DR InterPro; IPR025885; PapC_N.
DR InterPro; IPR037224; PapC_N_sf.
DR PANTHER; PTHR30451; PTHR30451; 1.
DR Pfam; PF13953; PapC_C; 1.
DR Pfam; PF13954; PapC_N; 1.
DR Pfam; PF00577; Usher; 1.
DR SUPFAM; SSF141729; SSF141729; 1.
DR PROSITE; PS01151; FIMBRIAL_USHER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Disulfide bond; Membrane; Plasmid;
KW Reference proteome; Signal; Transmembrane; Transmembrane beta strand;
KW Transport.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..833
FT /note="F1 capsule-anchoring protein"
FT /id="PRO_0000009300"
FT DISULFID 807..829
FT /evidence="ECO:0000255"
FT CONFLICT 366..367
FT /note="AL -> VF (in Ref. 1; CAA43968)"
FT /evidence="ECO:0000305"
FT CONFLICT 377
FT /note="L -> F (in Ref. 1; CAA43968)"
FT /evidence="ECO:0000305"
FT CONFLICT 392
FT /note="A -> E (in Ref. 1; CAA43968)"
FT /evidence="ECO:0000305"
FT CONFLICT 706
FT /note="N -> Y (in Ref. 1; CAA43968)"
FT /evidence="ECO:0000305"
FT HELIX 42..45
FT /evidence="ECO:0007829|PDB:4B0M"
FT STRAND 52..60
FT /evidence="ECO:0007829|PDB:4B0M"
FT STRAND 63..77
FT /evidence="ECO:0007829|PDB:4B0M"
FT STRAND 80..85
FT /evidence="ECO:0007829|PDB:4B0M"
FT HELIX 89..94
FT /evidence="ECO:0007829|PDB:4B0M"
FT HELIX 99..101
FT /evidence="ECO:0007829|PDB:4B0M"
FT HELIX 103..105
FT /evidence="ECO:0007829|PDB:4B0M"
FT STRAND 111..113
FT /evidence="ECO:0007829|PDB:4B0E"
FT HELIX 116..118
FT /evidence="ECO:0007829|PDB:4B0M"
FT STRAND 119..121
FT /evidence="ECO:0007829|PDB:4B0M"
FT STRAND 123..127
FT /evidence="ECO:0007829|PDB:4B0M"
FT TURN 128..131
FT /evidence="ECO:0007829|PDB:4B0M"
FT STRAND 132..136
FT /evidence="ECO:0007829|PDB:4B0M"
FT HELIX 139..141
FT /evidence="ECO:0007829|PDB:4B0M"
FT STRAND 147..150
FT /evidence="ECO:0007829|PDB:4B0M"
FT STRAND 261..263
FT /evidence="ECO:0007829|PDB:3FCG"
FT STRAND 266..286
FT /evidence="ECO:0007829|PDB:3FCG"
FT STRAND 288..294
FT /evidence="ECO:0007829|PDB:3FCG"
FT STRAND 296..301
FT /evidence="ECO:0007829|PDB:3FCG"
FT STRAND 311..318
FT /evidence="ECO:0007829|PDB:3FCG"
FT STRAND 324..331
FT /evidence="ECO:0007829|PDB:3FCG"
FT STRAND 746..754
FT /evidence="ECO:0007829|PDB:2XET"
FT STRAND 765..768
FT /evidence="ECO:0007829|PDB:2XET"
FT STRAND 782..789
FT /evidence="ECO:0007829|PDB:2XET"
FT STRAND 791..799
FT /evidence="ECO:0007829|PDB:2XET"
FT STRAND 805..812
FT /evidence="ECO:0007829|PDB:2XET"
FT STRAND 823..831
FT /evidence="ECO:0007829|PDB:2XET"
SQ SEQUENCE 833 AA; 93003 MW; 48F409F4E28F37BE CRC64;
MRYSKLFLCA GLTLATLPCW GRAYTFDSTM LDTNSGESID VSLFNQGLQL PGNYFVNVFV
NGRKVDSGNI DFRLEKHNGK ELLWPCLSSL QLTKYGIDID KYPDLIKSGT EQCVDLLAIP
HSDVQFYFNQ QKLSLIVPPQ ALLPRFDGIM PMQLWDDGIP ALFMNYNTNM QTRKFREGGK
SLDSYYAQLQ PGLNIGAWRF RSSTSWWKQQ GWQRSYIYAE RGLNTIKSRL TLGETYSDSS
IFDSIPIKGI KIASDESMVP YYQWNFAPVV RGIARTQARV EVLRDGYTVS NELVPSGPFE
LANLPLGGGS GELKVIIHES DGTKQVFTVP YDTPAVALRK GYFEYSMMGG EYRPANDLTQ
TSYVGALGMK YGLPRNLTLY GGLQGSQNYH AAALGIGAML GDFGAISTDV TQADSQKNKQ
KKESGQRWRV RYNKYLQSGT SLNIASEEYA TEGFNKLADT LNTYCKPNTR NDCRFDYAKP
KNKVQFNLSQ SIPGSGTLNF SGYRKNYWRD SRSTTSFSVG YNHFFRNGMS LTLNLSKTQN
INKYGEKTSE LLSNIWLSFP LSRWLGNNSI NSNYQMTSDS HGNTTHEVGV YGEAFDRQLY
WDVRERFNEK GRKYTSNALN LNYRGTYGEI SGNYSYDQTQ SQLGIGVNGN MVITQYGITA
GQKTGDTIAL VQAPDISGAS VGYWPGMKTD FRGYTNYGYL TPYRENKVEI NPVTLPNDAE
ITNNIVSVIP TKGAVVLAKF NARIGGRLFL HLKRSDNKPV PFGSIVTIEG QSSSSGIVGD
NSGVYLTGLP KKSKILVKWG RDKNQSCSSN VVLPEKTDIS GAYRLSTTCI LNN
