CAF1G_ARATH
ID CAF1G_ARATH Reviewed; 275 AA.
AC Q9SKZ2; Q949R6;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Probable CCR4-associated factor 1 homolog 7;
DE EC=3.1.13.4;
GN Name=CAF1-7; OrderedLocusNames=At2g32070; ORFNames=F22D22.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: Ubiquitous transcription factor required for a diverse set of
CC processes. It is a component of the CCR4 complex involved in the
CC control of gene expression (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage of poly(A) to 5'-AMP.; EC=3.1.13.4;
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250};
CC -!- SUBUNIT: Component of the CCR4-NOT complex, at least composed of CRR4
CC and CAF1 proteins. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the CAF1 family. {ECO:0000305}.
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DR EMBL; AC006223; AAD15397.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC08630.1; -; Genomic_DNA.
DR EMBL; AY050946; AAK93623.1; -; mRNA.
DR EMBL; AY142584; AAN13153.1; -; mRNA.
DR PIR; F84728; F84728.
DR RefSeq; NP_565735.1; NM_128767.4.
DR AlphaFoldDB; Q9SKZ2; -.
DR SMR; Q9SKZ2; -.
DR STRING; 3702.AT2G32070.1; -.
DR PaxDb; Q9SKZ2; -.
DR PRIDE; Q9SKZ2; -.
DR ProteomicsDB; 239175; -.
DR EnsemblPlants; AT2G32070.1; AT2G32070.1; AT2G32070.
DR GeneID; 817767; -.
DR Gramene; AT2G32070.1; AT2G32070.1; AT2G32070.
DR KEGG; ath:AT2G32070; -.
DR Araport; AT2G32070; -.
DR TAIR; locus:2045512; AT2G32070.
DR eggNOG; KOG0304; Eukaryota.
DR HOGENOM; CLU_027974_0_1_1; -.
DR InParanoid; Q9SKZ2; -.
DR OMA; HIREVWS; -.
DR OrthoDB; 931256at2759; -.
DR PhylomeDB; Q9SKZ2; -.
DR PRO; PR:Q9SKZ2; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SKZ2; baseline and differential.
DR Genevisible; Q9SKZ2; AT.
DR GO; GO:0030015; C:CCR4-NOT core complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000932; C:P-body; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004535; F:poly(A)-specific ribonuclease activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0043928; P:exonucleolytic catabolism of deadenylated mRNA; IBA:GO_Central.
DR Gene3D; 3.30.420.10; -; 1.
DR InterPro; IPR039637; CNOT7/CNOT8/Pop2.
DR InterPro; IPR006941; RNase_CAF1.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10797; PTHR10797; 1.
DR Pfam; PF04857; CAF1; 2.
DR SUPFAM; SSF53098; SSF53098; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Exonuclease; Hydrolase; Metal-binding; Nuclease; Nucleus;
KW Reference proteome; RNA-binding; Transcription; Transcription regulation.
FT CHAIN 1..275
FT /note="Probable CCR4-associated factor 1 homolog 7"
FT /id="PRO_0000371557"
FT BINDING 40
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 42
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 167
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
FT BINDING 236
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000250"
SQ SEQUENCE 275 AA; 31543 MW; 320745CB578F35B0 CRC64;
MSLFLKDDSI QIREVWNDNL ESEMALIREV VDDFPFVAMD TEFPGIVCRP VGTFKTNTEY
HYETLKTNVN ILKMIQLGLT FSDEKGNLPT CGTDNKYCIW QFNFREFDLE SDIYATDSIE
LLRQSGIDFV KNNEFGIDSK RFAELLMSSG IVLNENVHWV TFHSGYDFGY LLKLLTCQNL
PETQTGFFEM ISVYFPRVYD IKHLMKFCNS LHGGLNKLAE LLDVERVGIC HQAGSDSLLT
SCTFRKLQEN FFIGSMEKYS GVLYGLGVEN GQIVH
