ID   CAF1P_MAIZE             Reviewed;         674 AA.
AC   Q84N49;
DT   03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=CRS2-associated factor 1, chloroplastic;
DE   AltName: Full=Chloroplastic group IIA intron splicing facilitator CRS2-associated factor 1;
DE   Flags: Precursor;
GN   Name=CAF1;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION
RP   WITH CRS2.
RC   STRAIN=cv. B73;
RX   PubMed=12881426; DOI=10.1093/emboj/cdg372;
RA   Ostheimer G.J., Williams-Carrier R., Belcher S., Osborne E., Gierke J.,
RA   Barkan A.;
RT   "Group II intron splicing factors derived by diversification of an ancient
RT   RNA-binding domain.";
RL   EMBO J. 22:3919-3929(2003).
RN   [2]
RP   INTERACTION WITH CRS2.
RX   PubMed=16379013; DOI=10.1074/jbc.m508921200;
RA   Ostheimer G.J., Rojas M., Hadjivassiliou H., Barkan A.;
RT   "Formation of the CRS2-CAF2 group II intron splicing complex is mediated by
RT   a 22-amino acid motif in the COOH-terminal region of CAF2.";
RL   J. Biol. Chem. 281:4732-4738(2006).
CC   -!- FUNCTION: Required for the splicing of group IIB introns in
CC       chloroplasts. Forms splicing particles with CRS2. Interacts with RNA
CC       and confers intron specificity of the splicing particles.
CC       {ECO:0000269|PubMed:12881426}.
CC   -!- SUBUNIT: Interacts with CRS2 and RNA. Part of large ribonucleo-protein
CC       complexes that include group IIB introns, CRS2 and CAF1.
CC       {ECO:0000269|PubMed:12881426, ECO:0000269|PubMed:16379013}.
CC   -!- INTERACTION:
CC       Q84N49; B6TTV8: WTF1; NbExp=3; IntAct=EBI-15761664, EBI-15761679;
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC       {ECO:0000269|PubMed:12881426}.
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DR   EMBL; AY264368; AAP22135.1; -; mRNA.
DR   RefSeq; NP_001105220.1; NM_001111750.1.
DR   RefSeq; XP_008652328.1; XM_008654106.1.
DR   AlphaFoldDB; Q84N49; -.
DR   SMR; Q84N49; -.
DR   DIP; DIP-48740N; -.
DR   IntAct; Q84N49; 4.
DR   STRING; 4577.GRMZM2G173923_P01; -.
DR   PaxDb; Q84N49; -.
DR   PRIDE; Q84N49; -.
DR   EnsemblPlants; Zm00001eb306430_T001; Zm00001eb306430_P001; Zm00001eb306430.
DR   GeneID; 542118; -.
DR   Gramene; Zm00001eb306430_T001; Zm00001eb306430_P001; Zm00001eb306430.
DR   KEGG; zma:542118; -.
DR   MaizeGDB; 886554; -.
DR   eggNOG; ENOG502QQC4; Eukaryota.
DR   OMA; IVPSYME; -.
DR   OrthoDB; 408261at2759; -.
DR   Proteomes; UP000007305; Chromosome 7.
DR   ExpressionAtlas; Q84N49; baseline and differential.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR   GO; GO:0003729; F:mRNA binding; IEA:EnsemblPlants.
DR   GO; GO:0000373; P:Group II intron splicing; IEA:EnsemblPlants.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.110.60; -; 2.
DR   InterPro; IPR044599; CAF1P_plant.
DR   InterPro; IPR001890; RNA-binding_CRM.
DR   InterPro; IPR035920; YhbY-like_sf.
DR   PANTHER; PTHR46247; PTHR46247; 1.
DR   Pfam; PF01985; CRS1_YhbY; 2.
DR   SMART; SM01103; CRS1_YhbY; 2.
DR   SUPFAM; SSF75471; SSF75471; 2.
DR   PROSITE; PS51295; CRM; 2.
PE   1: Evidence at protein level;
KW   Chloroplast; mRNA processing; mRNA splicing; Plastid; Reference proteome;
KW   Repeat; Ribonucleoprotein; RNA-binding; Transit peptide.
FT   TRANSIT         1..54
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           55..674
FT                   /note="CRS2-associated factor 1, chloroplastic"
FT                   /id="PRO_0000283615"
FT   DOMAIN          183..279
FT                   /note="CRM 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT   DOMAIN          301..397
FT                   /note="CRM 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT   REGION          31..61
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          554..576
FT                   /note="CRS2 binding"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   674 AA;  75145 MW;  0C3454B196954E56 CRC64;
     MATARLPSRS FLSPAQQSYP RLPASVRLCL SHHEQPPTGP KRHRRAATSH PAFSAAARGR
     AKKIPIADTD EPAAGVRVTD RGISYRLDGA PFEFQYSYTE APRARPVALR EAPFMPFGPE
     ATPRPWTGRK PLPKSRKELP EFDSFVLPAP GKKGVKPVQS PGPFLAGMEP RYQSVSREDI
     LGEPLTKEEV SELVKGSLKS KRQLNMGRDG LTHNMLENIH SHWKRKRVCK IKCKGVCTID
     MDNICHQLEE KVGGKVIHRQ GGVIFLFRGR NYNYRTRPCF PLMLWKPVAP VYPRLVTKVP
     GGLTPDEATE MRTRGHQLPP ICKLGKNGVY ANLVNQVREA FEACDLVRVD CSGLNKSDCR
     KIGAKLKDLV PCILLSFEFE HILMWRGSDW KSSLPPLENS YEVTKVQESF SGKESNEKVT
     HSGNVLAQIE LVSAATSHKN WNLDEGQEKF KDSTDSDMVL NSAKDVPALF HSTGISRTEP
     SADIPLEYSP LNPVCDIMDP SLNCRSIPTN NCESRALVEK SEHCPDDYNL EMKRKRNDGT
     KGTVVLNSGS KVEGLLCLLE QAIHSGRALV LSEDELADSD LVYEKSVAFT KSIPRRLVFE
     NTRRKSSARR NVPDNHARTK THLVENKMLS SPVENKFIVN GGSAMQTIDH GQEFLSDVVH
     QGTLRVDELA KLLA