CAF2P_ORYSJ
ID CAF2P_ORYSJ Reviewed; 607 AA.
AC Q657G7; A0A0P0V224;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=CRS2-associated factor 2, chloroplastic;
DE AltName: Full=Chloroplastic group IIA intron splicing facilitator CRS2-associated factor 2;
DE Flags: Precursor;
GN OrderedLocusNames=Os01g0323300, LOC_Os01g21990;
GN ORFNames=OSJNBa0011P19.4, P0426D06.47;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Required for the splicing of group IIB introns in
CC chloroplasts. Forms splicing particles with CRS2. Interacts with RNA
CC and confers intron specificity of the splicing particles (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with CRS2 and RNA. Part of large ribonucleo-protein
CC complexes that include group IIB introns, CRS2 and CAF2 (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma {ECO:0000250}.
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DR EMBL; AP002953; BAD44991.1; -; Genomic_DNA.
DR EMBL; AP003211; BAD45045.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF04810.1; -; Genomic_DNA.
DR EMBL; AP014957; BAS71859.1; -; Genomic_DNA.
DR RefSeq; XP_015622825.1; XM_015767339.1.
DR AlphaFoldDB; Q657G7; -.
DR SMR; Q657G7; -.
DR STRING; 4530.OS01T0323300-00; -.
DR PaxDb; Q657G7; -.
DR PRIDE; Q657G7; -.
DR EnsemblPlants; Os01t0323300-00; Os01t0323300-00; Os01g0323300.
DR GeneID; 4326994; -.
DR Gramene; Os01t0323300-00; Os01t0323300-00; Os01g0323300.
DR KEGG; osa:4326994; -.
DR eggNOG; ENOG502QW9P; Eukaryota.
DR HOGENOM; CLU_012688_1_0_1; -.
DR InParanoid; Q657G7; -.
DR OMA; YSECPRA; -.
DR OrthoDB; 408261at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q657G7; OS.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000373; P:Group II intron splicing; IEA:InterPro.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.110.60; -; 2.
DR InterPro; IPR044599; CAF1P_plant.
DR InterPro; IPR001890; RNA-binding_CRM.
DR InterPro; IPR035920; YhbY-like_sf.
DR PANTHER; PTHR46247; PTHR46247; 1.
DR Pfam; PF01985; CRS1_YhbY; 2.
DR SMART; SM01103; CRS1_YhbY; 2.
DR SUPFAM; SSF75471; SSF75471; 2.
DR PROSITE; PS51295; CRM; 2.
PE 3: Inferred from homology;
KW Chloroplast; mRNA processing; mRNA splicing; Plastid; Reference proteome;
KW Repeat; Ribonucleoprotein; RNA-binding; Transit peptide.
FT TRANSIT 1..79
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 80..607
FT /note="CRS2-associated factor 2, chloroplastic"
FT /id="PRO_0000283621"
FT DOMAIN 228..324
FT /note="CRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT DOMAIN 346..442
FT /note="CRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT REGION 1..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 482..505
FT /note="CRS2 binding"
FT /evidence="ECO:0000250"
FT REGION 550..576
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..47
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 607 AA; 67876 MW; 667128CB8EEDE04C CRC64;
MSPPPPQRPS PSRAGRANLF SSPPPPLPNC YDPKHRRPAP PPLPSARRLP SNRRRRHDQP
PNPTTGNGGN PAFRAPHLRT AYRKPVPPVA AAGEGEALLA ADASDAADGR AVVVGPSGLS
FRLPGAPFDF RFSYSECPRA PPVAIREPAF LPFAPPTMPR PWTGKAPLLT KEEKARRRGV
RLHTPLGEEA PRTVSAHGIM MEVRGRRKLD LARVSPGDGR SREEVLGEPL TAAEVRDLVK
PHISHNRQLN IGRDGLTHNM LEMIHCHWRR QEICKVRCRG VPTVDMKNLC YHLEEKSGGK
VIHRVGGVVF LYRGRNYNPR TRPRYPLMLW KPATPVYPKL IQEAPEGLTK EEADEMRRRG
KDLLPICKLA KNGIYIYLVR DVRDAFEGSD LVKIDCEGLN PSDYKKIGAK LRDLVPCVLL
SFDNEQILMF RGKEWKSRYP KPLTLIPKIR KNNVPMSSDE SSSDEATDDD DRLAVREVLR
PKMFELWTNA IESSVALMLD DAEVDALTPD SLLTRVEDFS VTSQVVEHSF PAVLVANDES
NPDVLNAEYT EDEPETGTLE PQQHEFTESS DVAEDDHFED DMLKRLESSV PLGALPIDAV
VKQLNDE
