ID   UVRD1_MYCBO             Reviewed;         771 AA.
AC   P0A5A4; A0A1R3XZ05; P71561; X2BGI2;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=ATP-dependent DNA helicase UvrD1;
DE            EC=3.6.4.12;
GN   Name=uvrD1; Synonyms=ivrD; OrderedLocusNames=BQ2027_MB0974;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: DNA-dependent ATPase, acting on dsDNA with a 3'-ssDNA tail,
CC       unwinding with 3'-to 5'-polarity. Also highly efficient on nicked DNA.
CC       Involved in the post-incision events of nucleotide excision repair (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000305}.
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DR   EMBL; LT708304; SIT99572.1; -; Genomic_DNA.
DR   RefSeq; NP_854631.1; NC_002945.3.
DR   RefSeq; WP_003404859.1; NC_002945.4.
DR   AlphaFoldDB; P0A5A4; -.
DR   SMR; P0A5A4; -.
DR   EnsemblBacteria; SIT99572; SIT99572; BQ2027_MB0974.
DR   PATRIC; fig|233413.5.peg.1060; -.
DR   OMA; YQDTNRT; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR   Gene3D; 1.10.10.160; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR005751; ATP-dep_DNA_helicase_PcrA.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01073; pcrA; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding.
FT   CHAIN           1..771
FT                   /note="ATP-dependent DNA helicase UvrD1"
FT                   /id="PRO_0000102053"
FT   DOMAIN          21..311
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT   DOMAIN          312..603
FT                   /note="UvrD-like helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00617"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          691..716
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         45..50
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT   BINDING         309
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   771 AA;  85050 MW;  DBAA84E151F4E2C9 CRC64;
     MSVHATDAKP PGPSPADQLL DGLNPQQRQA VVHEGSPLLI VAGAGSGKTA VLTRRIAYLM
     AARGVGVGQI LAITFTNKAA AEMRERVVGL VGEKARYMWV STFHSTCVRI LRNQAALIEG
     LNSNFSIYDA DDSRRLLQMV GRDLGLDIKR YSPRLLANAI SNLKNELIDP HQALAGLTED
     SDDLARAVAS VYDEYQRRLR AANALDFDDL IGETVAVLQA FPQIAQYYRR RFRHVLVDEY
     QDTNHAQYVL VRELVGRDSN DGIPPGELCV VGDADQSIYA FRGATIRNIE DFERDYPDTR
     TILLEQNYRS TQNILSAANS VIARNAGRRE KRLWTDAGAG ELIVGYVADN EHDEARFVAE
     EIDALAEGSE ITYNDVAVFY RTNNSSRSLE EVLIRAGIPY KVVGGVRFYE RKEIRDIVAY
     LRVLDNPGDA VSLRRILNTP RRGIGDRAEA CVAVYAENTG VGFGDALVAA AQGKVPMLNT
     RAEKAIAGFV EMFDELRGRL DDDLGELVEA VLERTGYRRE LEASTDPQEL ARLDNLNELV
     SVAHEFSTDR ENAAALGPDD EDVPDTGVLA DFLERVSLVA DADEIPEHGA GVVTLMTLHT
     AKGLEFPVVF VTGWEDGMFP HMRALDNPTE LSEERRLAYV GITRARQRLY VSRAIVRSSW
     GQPMLNPESR FLREIPQELI DWRRTAPKPS FSAPVSGAGR FGSARPSPTR SGASRRPLLV
     LQVGDRVTHD KYGLGRVEEV SGVGESAMSL IDFGSSGRVK LMHNHAPVTK L