ID   UVRD1_MYCLE             Reviewed;         778 AA.
AC   Q9CD72;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=ATP-dependent DNA helicase UvrD1;
DE            EC=3.6.4.12;
GN   Name=uvrD; OrderedLocusNames=ML0153;
OS   Mycobacterium leprae (strain TN).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium.
OX   NCBI_TaxID=272631;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=TN;
RX   PubMed=11234002; DOI=10.1038/35059006;
RA   Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R.,
RA   Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E.,
RA   Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R.,
RA   Davies R.M., Devlin K., Duthoy S., Feltwell T., Fraser A., Hamlin N.,
RA   Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S.,
RA   Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M.,
RA   Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R.,
RA   Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R.,
RA   Barrell B.G.;
RT   "Massive gene decay in the leprosy bacillus.";
RL   Nature 409:1007-1011(2001).
CC   -!- FUNCTION: DNA-dependent ATPase, acting on dsDNA with a 3'-ssDNA tail,
CC       unwinding with 3'-to 5'-polarity. Also highly efficient on nicked DNA.
CC       Involved in the post-incision events of nucleotide excision repair (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AL583917; CAC29661.1; -; Genomic_DNA.
DR   PIR; A86928; A86928.
DR   RefSeq; NP_301239.1; NC_002677.1.
DR   RefSeq; WP_010907564.1; NC_002677.1.
DR   AlphaFoldDB; Q9CD72; -.
DR   SMR; Q9CD72; -.
DR   STRING; 272631.ML0153; -.
DR   EnsemblBacteria; CAC29661; CAC29661; CAC29661.
DR   KEGG; mle:ML0153; -.
DR   PATRIC; fig|272631.5.peg.237; -.
DR   Leproma; ML0153; -.
DR   eggNOG; COG0210; Bacteria.
DR   HOGENOM; CLU_004585_5_2_11; -.
DR   OMA; YQDTNRT; -.
DR   Proteomes; UP000000806; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.160; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070; PTHR11070; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..778
FT                   /note="ATP-dependent DNA helicase UvrD1"
FT                   /id="PRO_0000102054"
FT   DOMAIN          19..313
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT   DOMAIN          314..610
FT                   /note="UvrD-like helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00617"
FT   BINDING         43..48
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT   BINDING         311
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   778 AA;  85635 MW;  3964DF128E008EF6 CRC64;
     MSVHTTDAKP DSEVDRLLDG LNPQQRQAVV HEGSPLLIVA GAGSGKTTVL ARRIAYLIAA
     RSVGVSQILA ITFTNKAAAE MRERVARLVG DHTGPSMWVS TFHSTCVRIL RNQASLIGGL
     NSNFSIYDVD DSRSLLQMIG QDMGLDIKRY SPRLMANAIS NLKNELIDAE SAVANLSSDT
     DDLARTVATV YGEYQRRLRT ANALDFDDLI GETVGILQAF PQIAEHYRRR FRHVLVDEYQ
     DTNHAQYVLV RELVGHGARN SPDDMPPGEL CVVGDADQSI YAFRGSTISN IEDFERDYPD
     TTTILLEQNY RSTQNILSAA NSVIARNFGR RDKRLWTDAG KGELIVGYVS DNEHDEAKFI
     ADEIDALVGG GEITYDDVAV LYRANNLSRS LEEVFIPTGI PYKVVGGFCF YESKEIRDLI
     AYLRVLDNPG DAVSMRRILN TPRRGIGDRA EACVSVYAEN TGTSFADALQ AVAEGKVPML
     NTRSVKAIAG FVALLDDLRC RVDDDLGELV ESVLERSGYL RELESSTDPQ ELARLDNLNE
     LVSFAHEFST EQANAAALAK SLHTPEDEDV PDTGALAAFL EKVSLMSDTD QIPENNSGVV
     TLMTLHAAKG LEFPVVFVTG WEDGMLPHMR TLGDPTELSE ERRLAYVGIT RARQRLYLSR
     AITRSSWGQP ILNPESRFLR EIPPELIDWR RSILTDSYST PASGASRFGR VRPSSIRSGA
     SKRALLVLAP GDRVTHDKYG LGRVEEVSGV GESAISLIDF GSSGRIKLMH NHAPVTKL