UVRD_SALTY
ID UVRD_SALTY Reviewed; 720 AA.
AC Q05311; Q9L6P3;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 29-AUG-2001, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=DNA helicase II;
DE EC=3.6.4.12;
GN Name=uvrD; OrderedLocusNames=STM3951; ORFNames=STMD1.39;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 708-720.
RX PubMed=8314774; DOI=10.1016/s0021-9258(19)85210-9;
RA Smith R.L., Banks J.L., Snavely M.D., Maguire M.E.;
RT "Sequence and topology of the CorA magnesium transport systems of
RT Salmonella typhimurium and Escherichia coli. Identification of a new class
RT of transport protein.";
RL J. Biol. Chem. 268:14071-14080(1993).
CC -!- FUNCTION: Has both ATPase and helicase activities. Unwinds DNA duplexes
CC with 3' to 5' polarity with respect to the bound strand and initiates
CC unwinding most effectively when a single-stranded region is present.
CC Involved in the post-incision events of nucleotide excision repair and
CC methyl-directed mismatch repair.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000305}.
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DR EMBL; AF233324; AAF33441.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL22795.1; -; Genomic_DNA.
DR EMBL; L11043; AAA02965.1; -; Unassigned_DNA.
DR RefSeq; NP_462836.1; NC_003197.2.
DR RefSeq; WP_000383441.1; NC_003197.2.
DR AlphaFoldDB; Q05311; -.
DR SMR; Q05311; -.
DR STRING; 99287.STM3951; -.
DR PaxDb; Q05311; -.
DR EnsemblBacteria; AAL22795; AAL22795; STM3951.
DR GeneID; 1255477; -.
DR KEGG; stm:STM3951; -.
DR PATRIC; fig|99287.12.peg.4169; -.
DR HOGENOM; CLU_004585_5_5_6; -.
DR OMA; YQDTNRT; -.
DR PhylomeDB; Q05311; -.
DR BioCyc; SENT99287:STM3951-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0033202; C:DNA helicase complex; IBA:GO_Central.
DR GO; GO:0043138; F:3'-5' DNA helicase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:InterPro.
DR GO; GO:0000725; P:recombinational repair; IBA:GO_Central.
DR GO; GO:0009432; P:SOS response; IEA:UniProtKB-KW.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR005753; DNA_helicase_ATP-dep_UvrD.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070; PTHR11070; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01075; uvrD; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA damage; DNA repair; DNA replication; DNA-binding;
KW Helicase; Hydrolase; Nucleotide-binding; Reference proteome; SOS response.
FT CHAIN 1..720
FT /note="DNA helicase II"
FT /id="PRO_0000102073"
FT DOMAIN 8..286
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT DOMAIN 287..564
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00617"
FT BINDING 32..37
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00560"
FT BINDING 284
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 720 AA; 81981 MW; CE6D60296392511C CRC64;
MDVSYLLDSL NDKQREAVAA PRSNMLVLAG AGSGKTRVLV HRIAWLLSVE NNSPYSIMAV
TFTNKAAAEM RHRIGQLMGT SQGGMWVGTF HGLAHRLLRA HHMDANLPQD FQILDSEDQM
RLLKRLIKAM NLDEKQWPPR QAMWYINSQK DEGLRPHHIQ SYGNPVEQTW QKVYQAYQEA
CDRAGLVDFA ELLLRAHELW LNKPHILQHY RERFTNILVD EFQDTNNIQY AWVRLLAGDT
GKVMIVGDDD QSIYGWRGAQ VENIQRFLND FPGAQTIRLE QNYRSTSNIL SAANALIENN
NGRLGKKLWT DGVDGEPISL YCAFNELDEA RFVVNRIKTW QDNGGALAQC AILYRSNAQS
RVLEEALLQA SMPYRIYGGM RFFERQEIKD ALSYLRLIAN RNDDAAFERV VNTPTRGIGD
RTLDVVRQTS RDRQLTLWQV CRELLQEKAL AGRAASALQR FMELIDALAQ ETADMPLHVQ
TDRVIKDSGL RTMYEQEKGE KGQTRIENLE ELVTATRQFS YNDEDEDLMP LQAFLSHAAL
EAGEGQADTW QDAVQLMTLH SAKGLEFPQV FIVGMEEGMF PSQMSLDEGG RLEEERRLAY
VGVTRAMQKL TLTYAETRRL YGKEVYHRPS RFIGELPEEC VEEVRLRATV SRPVSHQRMG
TPLAENDTGY KLGQRVRHAK FGEGTIVNLE GSGEHSRLQV AFQGQGIKWL VAAYAKLETV
