UVSSA_RAT
ID UVSSA_RAT Reviewed; 720 AA.
AC D3ZND0;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 2.
DT 03-AUG-2022, entry version 64.
DE RecName: Full=UV-stimulated scaffold protein A;
GN Name=Uvssa;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RX PubMed=15057822; DOI=10.1038/nature02426;
RA Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J.,
RA Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G.,
RA Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G.,
RA Morgan M., Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G.,
RA Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S.,
RA Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T.,
RA Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D.,
RA Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L.,
RA Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D.,
RA Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M.,
RA Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C.,
RA Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J.,
RA Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H.,
RA Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X.,
RA Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q.,
RA Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P.,
RA Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A.,
RA Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C.,
RA Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J.,
RA Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J.,
RA Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F.,
RA Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A.,
RA Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A.,
RA Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J.,
RA Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E.,
RA Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M.,
RA Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C.,
RA Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L.,
RA Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W.,
RA Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y.,
RA Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V.,
RA Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M.,
RA Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S.,
RA Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B.,
RA Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R.,
RA Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J.,
RA Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D.,
RA Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S.,
RA Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S.,
RA Mockrin S., Collins F.S.;
RT "Genome sequence of the Brown Norway rat yields insights into mammalian
RT evolution.";
RL Nature 428:493-521(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Brown Norway;
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 596-720.
RC STRAIN=Sprague-Dawley;
RX PubMed=8889548; DOI=10.1101/gr.6.9.791;
RA Bonaldo M.F., Lennon G., Soares M.B.;
RT "Normalization and subtraction: two approaches to facilitate gene
RT discovery.";
RL Genome Res. 6:791-806(1996).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-406, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Factor involved in transcription-coupled nucleotide excision
CC repair (TC-NER) in response to UV damage. TC-NER allows RNA polymerase
CC II-blocking lesions to be rapidly removed from the transcribed strand
CC of active genes. Acts by promoting stabilization of ERCC6 by recruiting
CC deubiquitinating enzyme USP7 to TC-NER complexes, preventing UV-induced
CC degradation of ERCC6 by the proteasome. Interacts with the elongating
CC form of RNA polymerase II (RNA pol IIo) and facilitates its
CC ubiquitination at UV damage sites, leading to promote RNA pol IIo
CC backtracking to allow access to the nucleotide excision repair
CC machinery. Not involved in processing oxidative damage (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with the elongating form of RNA polymerase II (RNA
CC pol IIo). Interacts with ERCC6, ERCC8 and USP7 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000250}. Note=Accumulates at UV
CC DNA damage sites. {ECO:0000250}.
CC -!- PTM: Monoubiquitinated: ubiquitination does not increase in response to
CC UV. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the UVSSA family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EDM00129.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; CH473963; EDM00129.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BE096344; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; NP_001128030.1; NM_001134558.1.
DR RefSeq; XP_006251383.1; XM_006251321.3.
DR RefSeq; XP_006251385.1; XM_006251323.3.
DR RefSeq; XP_006251386.1; XM_006251324.3.
DR RefSeq; XP_006251387.1; XM_006251325.3.
DR AlphaFoldDB; D3ZND0; -.
DR SMR; D3ZND0; -.
DR STRING; 10116.ENSRNOP00000006841; -.
DR iPTMnet; D3ZND0; -.
DR PhosphoSitePlus; D3ZND0; -.
DR PaxDb; D3ZND0; -.
DR PeptideAtlas; D3ZND0; -.
DR PRIDE; D3ZND0; -.
DR Ensembl; ENSRNOT00000006841; ENSRNOP00000006841; ENSRNOG00000005122.
DR GeneID; 314061; -.
DR KEGG; rno:314061; -.
DR UCSC; RGD:1306371; rat.
DR CTD; 57654; -.
DR RGD; 1306371; Uvssa.
DR eggNOG; KOG2374; Eukaryota.
DR GeneTree; ENSGT00390000000377; -.
DR InParanoid; D3ZND0; -.
DR OrthoDB; 996127at2759; -.
DR TreeFam; TF321660; -.
DR Reactome; R-RNO-6781823; Formation of TC-NER Pre-Incision Complex.
DR Reactome; R-RNO-6782135; Dual incision in TC-NER.
DR Reactome; R-RNO-6782210; Gap-filling DNA repair synthesis and ligation in TC-NER.
DR PRO; PR:D3ZND0; -.
DR Proteomes; UP000002494; Chromosome 14.
DR Proteomes; UP000234681; Chromosome 14.
DR GO; GO:0005694; C:chromosome; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0000993; F:RNA polymerase II complex binding; ISS:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB.
DR GO; GO:0009411; P:response to UV; ISS:UniProtKB.
DR GO; GO:0006283; P:transcription-coupled nucleotide-excision repair; ISS:UniProtKB.
DR Gene3D; 1.25.40.90; -; 1.
DR InterPro; IPR008942; ENTH_VHS.
DR InterPro; IPR018610; UVSSA.
DR PANTHER; PTHR28670; PTHR28670; 1.
DR Pfam; PF09740; DUF2043; 1.
DR SUPFAM; SSF48464; SSF48464; 1.
PE 1: Evidence at protein level;
KW Chromosome; Coiled coil; DNA damage; DNA repair; Phosphoprotein;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..720
FT /note="UV-stimulated scaffold protein A"
FT /id="PRO_0000417994"
FT REGION 2..145
FT /note="VHS-like"
FT REGION 234..253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 269..304
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 392..415
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 451..507
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 598..637
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 649..669
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 169..198
FT /evidence="ECO:0000255"
FT COMPBIAS 280..304
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 451..468
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 473..496
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 598..625
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 294
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q2YD98"
FT MOD_RES 406
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT CONFLICT 600..601
FT /note="KI -> NM (in Ref. 3; BE096344)"
FT /evidence="ECO:0000305"
FT CONFLICT 605
FT /note="D -> G (in Ref. 3; BE096344)"
FT /evidence="ECO:0000305"
FT CONFLICT 611
FT /note="L -> F (in Ref. 3; BE096344)"
FT /evidence="ECO:0000305"
FT CONFLICT 617
FT /note="A -> V (in Ref. 3; BE096344)"
FT /evidence="ECO:0000305"
FT CONFLICT 624..625
FT /note="LQ -> VL (in Ref. 3; BE096344)"
FT /evidence="ECO:0000305"
FT CONFLICT 635
FT /note="D -> G (in Ref. 3; BE096344)"
FT /evidence="ECO:0000305"
FT CONFLICT 645
FT /note="A -> S (in Ref. 3; BE096344)"
FT /evidence="ECO:0000305"
FT CONFLICT 653
FT /note="S -> F (in Ref. 3; BE096344)"
FT /evidence="ECO:0000305"
FT CONFLICT 665
FT /note="K -> N (in Ref. 3; BE096344)"
FT /evidence="ECO:0000305"
FT CONFLICT 676..678
FT /note="ANT -> SNI (in Ref. 3; BE096344)"
FT /evidence="ECO:0000305"
FT CONFLICT 708
FT /note="H -> P (in Ref. 3; BE096344)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 720 AA; 82035 MW; 2EB57E65A86E80F5 CRC64;
MDQKLSQLIE ELTTSGESQL NAQKMKELKK ICKSSEEQLS HAYRLLMTQL TQDHAEIRLS
AFQIVDELFT RSHQFRVLLV SDFQEFLELT LGTDNDHPLP PPREAAQRLR QAAMQAVEGW
NEKFGEAYKK LALGYHFLKH TKKVDFRDIN VRTLAERKRE EEKQKHLDKI HRESADRAKR
EMEEMSDEIG CCLTEVENCF RLLVPLDLGP YREDKFFGEA SGIAEDHAPC AWSPDLATPR
GSGLSGPQDE EQPCCSKDLV ASAHHAGSAV GLKAPAPAAT EDPCRDEDRH SEHSDPEDFL
RSHGLGSHKY TLDVELPSDG LKVQENEDNL AVLHAARDSL KLIQNKFLPA VCSWVQRFTR
AGIYSGHLKQ AIDLKMELEL ALKKYEELNI EPGRGQRSRT EALEDSEEED QDFVEVPEKE
GYEPRIPDHL RAEYGLEPKA PLKTLEKHTA VCSVQERTRR RREEEASDPT SAAAQMLRLQ
DCLSSPSSSS TRGPLGPEEA QKQAERARAP MVPFGVDLCY WGQEQLTAGK ILKSDSQHRF
WKPHEVEEEV DSAHVSEMLH SRHITFSGKF EPVQHKCRAL RPNGRLCERQ DRLKCPFHGK
IIPRDDKGQP LNPEDRAREQ RQQLQQQRAH PDWQDPEFMK DVEAATGVDL GSSKYSKKGK
GKKKKHPNLT DLRERANTAR ARLEKKVFAK GAVQRVVAAM NQMDQKKHEK FANQFNYALK
