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UVSW_BPT4
ID   UVSW_BPT4               Reviewed;         587 AA.
AC   P20703;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 2.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=ATP-dependent DNA helicase uvsW;
DE            EC=3.6.4.12;
DE   AltName: Full=Dar protein;
GN   Name=uvsW; Synonyms=dar;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS, AND CHARACTERIZATION.
RX   PubMed=9233823; DOI=10.1093/emboj/16.13.4142;
RA   Carles-Kinch K., George J.W., Kreuzer K.N.;
RT   "Bacteriophage T4 UvsW protein is a helicase involved in recombination,
RT   repair and the regulation of DNA replication origins.";
RL   EMBO J. 16:4142-4151(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-139.
RX   PubMed=2377482; DOI=10.1093/nar/18.14.4277;
RA   Kaliman A.V., Khasanova M.A., Kryukov V.M., Tanyashin V.I., Bayev A.A.;
RT   "The nucleotide sequence of the region of bacteriophage T4 inh(lip)-hoc
RT   genes.";
RL   Nucleic Acids Res. 18:4277-4277(1990).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15.
RX   PubMed=2388264; DOI=10.1016/0022-2836(90)90283-r;
RA   Derr L.K., Kreuzer K.N.;
RT   "Expression and function of the uvsW gene of bacteriophage T4.";
RL   J. Mol. Biol. 214:643-656(1990).
RN   [5]
RP   FUNCTION.
RX   PubMed=24013402; DOI=10.1038/ncomms3368;
RA   Manosas M., Perumal S.K., Bianco P., Ritort F., Benkovic S.J.,
RA   Croquette V.;
RT   "RecG and UvsW catalyse robust DNA rewinding critical for stalled DNA
RT   replication fork rescue.";
RL   Nat. Commun. 4:2368-2368(2013).
RN   [6]
RP   FUNCTION, AND INTERACTION WITH GP32.
RX   PubMed=23732982; DOI=10.1016/j.jmb.2013.05.012;
RA   Perumal S.K., Nelson S.W., Benkovic S.J.;
RT   "Interaction of T4 UvsW helicase and single-stranded DNA binding protein
RT   gp32 through its carboxy-terminal acidic tail.";
RL   J. Mol. Biol. 425:2823-2839(2013).
RN   [7]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-282.
RX   PubMed=15062081; DOI=10.1016/j.str.2004.02.016;
RA   Sickmier E.A., Kreuzer K.N., White S.W.;
RT   "The crystal structure of the UvsW helicase from bacteriophage T4.";
RL   Structure 12:583-592(2004).
CC   -!- FUNCTION: Plays important roles in recombination-dependent DNA repair
CC       and the reorganization of stalled replication forks during viral DNA
CC       synthesis. {ECO:0000269|PubMed:23732982, ECO:0000269|PubMed:24013402}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBUNIT: Interacts with gp32. {ECO:0000269|PubMed:23732982}.
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DR   EMBL; AF158101; AAD42668.1; -; Genomic_DNA.
DR   EMBL; X56097; CAA39576.1; -; Genomic_DNA.
DR   EMBL; X14869; CAA33014.1; -; Genomic_DNA.
DR   PIR; PQ0097; S10498.
DR   RefSeq; NP_049796.1; NC_000866.4.
DR   PDB; 1RIF; X-ray; 2.00 A; A/B=1-282.
DR   PDB; 2JPN; NMR; -; A=512-587.
DR   PDB; 2OCA; X-ray; 2.70 A; A=1-502.
DR   PDBsum; 1RIF; -.
DR   PDBsum; 2JPN; -.
DR   PDBsum; 2OCA; -.
DR   BMRB; P20703; -.
DR   SMR; P20703; -.
DR   PRIDE; P20703; -.
DR   GeneID; 1258733; -.
DR   KEGG; vg:1258733; -.
DR   EvolutionaryTrace; P20703; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004386; F:helicase activity; IDA:CACAO.
DR   Gene3D; 1.20.1280.210; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR001650; Helicase_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR020975; UvsW.1_dom.
DR   InterPro; IPR038341; UvsW_sf.
DR   Pfam; PF00271; Helicase_C; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   Pfam; PF11637; UvsW-1; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00490; HELICc; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
DR   PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; Helicase; Hydrolase; Late protein;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..587
FT                   /note="ATP-dependent DNA helicase uvsW"
FT                   /id="PRO_0000164988"
FT   DOMAIN          122..280
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   DOMAIN          335..501
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT   BINDING         135..142
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT   MUTAGEN         141
FT                   /note="K->R: Loss of activity."
FT                   /evidence="ECO:0000269|PubMed:9233823"
FT   STRAND          3..8
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   TURN            9..11
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   STRAND          12..16
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           19..28
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   STRAND          30..32
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   TURN            34..37
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           40..43
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   STRAND          49..54
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   STRAND          59..61
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           62..67
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           68..74
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   STRAND          79..81
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           83..86
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           93..101
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   STRAND          105..107
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   STRAND          110..112
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   HELIX           116..128
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   STRAND          129..133
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   TURN            138..140
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   HELIX           141..155
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   STRAND          157..163
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           167..179
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           185..187
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   STRAND          188..190
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           192..194
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   HELIX           199..201
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   STRAND          206..210
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           212..215
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           220..225
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   STRAND          226..232
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           234..236
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           239..245
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   TURN            246..248
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   STRAND          254..258
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   HELIX           262..264
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   HELIX           269..276
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   STRAND          278..280
FT                   /evidence="ECO:0007829|PDB:1RIF"
FT   STRAND          298..305
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   HELIX           308..314
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   HELIX           319..327
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   HELIX           330..344
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   TURN            345..347
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   STRAND          349..356
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   HELIX           357..368
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   STRAND          372..380
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   HELIX           383..395
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   STRAND          400..404
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   HELIX           405..410
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   STRAND          416..424
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   HELIX           431..440
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   STRAND          450..457
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   STRAND          463..466
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   HELIX           476..490
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   STRAND          495..501
FT                   /evidence="ECO:0007829|PDB:2OCA"
FT   STRAND          514..517
FT                   /evidence="ECO:0007829|PDB:2JPN"
FT   HELIX           518..523
FT                   /evidence="ECO:0007829|PDB:2JPN"
FT   HELIX           528..533
FT                   /evidence="ECO:0007829|PDB:2JPN"
FT   HELIX           538..551
FT                   /evidence="ECO:0007829|PDB:2JPN"
FT   TURN            552..554
FT                   /evidence="ECO:0007829|PDB:2JPN"
FT   HELIX           560..578
FT                   /evidence="ECO:0007829|PDB:2JPN"
FT   TURN            583..585
FT                   /evidence="ECO:0007829|PDB:2JPN"
SQ   SEQUENCE   587 AA;  67525 MW;  D7FBD8CE1E38C7CA CRC64;
     MDIKVHFHDF SHVRIDCEES TFHELRDFFS FEADGYRFNP RFRYGNWDGR IRLLDYNRLL
     PFGLVGQIKK FCDNFGYKAW IDPQINEKEE LSRKDFDEWL SKLEIYSGNK RIEPHWYQKD
     AVFEGLVNRR RILNLPTSAG KSLIQALLAR YYLENYEGKI LIIVPTTALT TQMADDFVDY
     RLFSHAMIKK IGGGASKDDK YKNDAPVVVG TWQTVVKQPK EWFSQFGMMM NDECHLATGK
     SISSIISGLN NCMFKFGLSG SLRDGKANIM QYVGMFGEIF KPVTTSKLME DGQVTELKIN
     SIFLRYPDEF TTKLKGKTYQ EEIKIITGLS KRNKWIAKLA IKLAQKDENA FVMFKHVSHG
     KAIFDLIKNE YDKVYYVSGE VDTETRNIMK TLAENGKGII IVASYGVFST GISVKNLHHV
     VLAHGVKSKI IVLQTIGRVL RKHGSKTIAT VWDLIDSAGV KPKSANTKKK YVHLNYLLKH
     GIDRIQRYAD EKFNYVMKTV NLISFGPLEK KMLLEFKQFL YEASIDEFMG KIASCQTLEG
     LEELEAYYKK RVKETELKDT DDISVRDALA GKRAELEDSD DEVEESF
 
 
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