ID   UVSY_BPT4               Reviewed;         137 AA.
AC   P04537;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 3.
DT   23-FEB-2022, entry version 81.
DE   RecName: Full=Recombination protein uvsY;
GN   Name=uvsY;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1871975; DOI=10.1016/0042-6822(91)90852-3;
RA   Gruidl M.E., Chen T.C., Gargano S., Storlazzi A., Cascino A., Mosig G.;
RT   "Two bacteriophage T4 base plate genes (25 and 26) and the DNA repair gene
RT   uvsY belong to spatially and temporally overlapping transcription units.";
RL   Virology 184:359-369(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3412904; DOI=10.1093/nar/16.15.7729;
RA   Kobayashi M., Saito H., Takahashi H.;
RT   "Confirmation of the reading frame of bacteriophage T4 uvsY gene.";
RL   Nucleic Acids Res. 16:7729-7729(1988).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3000071; DOI=10.1016/0042-6822(85)90137-0;
RA   Takahashi H., Kobayashi M., Noguchi T., Saito H.;
RT   "Nucleotide sequence of bacteriophage T4 uvsY gene.";
RL   Virology 147:349-353(1985).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3026891; DOI=10.1093/genetics/114.4.1061;
RA   Gruidl M.E., Mosig G.;
RT   "Sequence and transcripts of the bacteriophage T4 DNA repair gene uvsY.";
RL   Genetics 114:1061-1079(1986).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [6]
RP   INTERACTION WITH GP32.
RX   PubMed=9893989; DOI=10.1021/bi9817055;
RA   Sweezy M.A., Morrical S.W.;
RT   "Biochemical interactions within a ternary complex of the bacteriophage T4
RT   recombination proteins uvsY and gp32 bound to single-stranded DNA.";
RL   Biochemistry 38:936-944(1999).
RN   [7]
RP   SUBUNIT.
RX   PubMed=9548953; DOI=10.1021/bi9800956;
RA   Beernink H.T., Morrical S.W.;
RT   "The uvsY recombination protein of bacteriophage T4 forms hexamers in the
RT   presence and absence of single-stranded DNA.";
RL   Biochemistry 37:5673-5681(1998).
RN   [8]
RP   FUNCTION.
RX   PubMed=18565541; DOI=10.1016/j.jmb.2008.05.039;
RA   Pant K., Shokri L., Karpel R.L., Morrical S.W., Williams M.C.;
RT   "Modulation of T4 gene 32 protein DNA binding activity by the recombination
RT   mediator protein UvsY.";
RL   J. Mol. Biol. 380:799-811(2008).
RN   [9]
RP   SUBUNIT, DNA-BINDING, AND FUNCTION.
RX   PubMed=20371513; DOI=10.1093/nar/gkq219;
RA   Xu H., Beernink H.T., Morrical S.W.;
RT   "DNA-binding properties of T4 UvsY recombination mediator protein:
RT   polynucleotide wrapping promotes high-affinity binding to single-stranded
RT   DNA.";
RL   Nucleic Acids Res. 38:4821-4833(2010).
CC   -!- FUNCTION: Plays a role in viral DNA synthesis by promoting enzymatic
CC       activities of UvsX recombinase, by promoting UvsX-ssDNA filament
CC       assembly, and by helping UvsX to displace bound gp32 from ssDNA.
CC       {ECO:0000269|PubMed:18565541, ECO:0000269|PubMed:20371513}.
CC   -!- SUBUNIT: Homohexamer. Interacts with gp32.
CC       {ECO:0000269|PubMed:20371513, ECO:0000269|PubMed:9548953,
CC       ECO:0000269|PubMed:9893989}.
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DR   EMBL; M77695; AAA32550.1; -; Genomic_DNA.
DR   EMBL; M11495; AAA32546.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; X05134; CAA28779.1; -; Genomic_DNA.
DR   EMBL; X04856; CAA28549.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42670.2; -; Genomic_DNA.
DR   PIR; JS0136; ZXBPT4.
DR   RefSeq; NP_049799.2; NC_000866.4.
DR   PDB; 4ZWQ; X-ray; 2.35 A; A/B/C/D/E/F/G=1-137.
DR   PDB; 4ZWR; X-ray; 3.39 A; A/B/C/D/E/F/G=2-137.
DR   PDB; 4ZWS; X-ray; 2.60 A; A/B/C/D/E/F/G=1-137.
DR   PDB; 4ZWT; X-ray; 4.20 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N=1-137.
DR   PDBsum; 4ZWQ; -.
DR   PDBsum; 4ZWR; -.
DR   PDBsum; 4ZWS; -.
DR   PDBsum; 4ZWT; -.
DR   SMR; P04537; -.
DR   PRIDE; P04537; -.
DR   GeneID; 1258547; -.
DR   KEGG; vg:1258547; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0071897; P:DNA biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   InterPro; IPR021289; UvsY.
DR   Pfam; PF11056; UvsY; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA recombination; DNA synthesis; DNA-binding;
KW   Reference proteome.
FT   CHAIN           1..137
FT                   /note="Recombination protein uvsY"
FT                   /id="PRO_0000164989"
FT   CONFLICT        113
FT                   /note="A -> R (in Ref. 1; AAA32550)"
FT                   /evidence="ECO:0000305"
FT   HELIX           3..14
FT                   /evidence="ECO:0007829|PDB:4ZWQ"
FT   HELIX           18..20
FT                   /evidence="ECO:0007829|PDB:4ZWQ"
FT   HELIX           21..64
FT                   /evidence="ECO:0007829|PDB:4ZWQ"
FT   HELIX           80..88
FT                   /evidence="ECO:0007829|PDB:4ZWQ"
FT   HELIX           91..134
FT                   /evidence="ECO:0007829|PDB:4ZWQ"
SQ   SEQUENCE   137 AA;  15839 MW;  63BB7A96AA7C3E66 CRC64;
     MRLEDLQEEL KKDVFIDSTK LQYEAANNVM LYSKWLNKHS SIKKEMLRIE AQKKVALKAR
     LDYYSGRGDG DEFSMDRYEK SEMKTVLSAD KDVLKVDTSL QYWGILLDFC SGALDAIKSR
     GFAIKHIQDM RAFEAGK