ID   CAGA_STRGL              Reviewed;         143 AA.
AC   Q06110;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Antitumor antibiotic C-1027 apoprotein;
DE   AltName: Full=C-1027-AG;
DE   Flags: Precursor;
GN   Name=cagA;
OS   Streptomyces globisporus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1908;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=C-1027;
RX   PubMed=1369059; DOI=10.1271/bbb.56.1592;
RA   Sakata N., Ikeno S., Hori M., Hamada M., Otani T.;
RT   "Cloning and nucleotide sequencing of the antitumor antibiotic C-1027
RT   apoprotein gene.";
RL   Biosci. Biotechnol. Biochem. 56:1592-1595(1992).
RN   [2]
RP   PROTEIN SEQUENCE OF 34-143.
RX   PubMed=1368692; DOI=10.1271/bbb1961.55.407;
RA   Otani T., Yasuhara T., Minami Y., Shimazu T., Zhang R., Xie M.Y.;
RT   "Purification and primary structure of C-1027-AG, a selective antagonist of
RT   antitumor antibiotic C-1027, from Streptomyces globisporus.";
RL   Agric. Biol. Chem. 55:407-417(1991).
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=8504075; DOI=10.1021/bi00072a008;
RA   Sugiura Y., Matsumoto T.;
RT   "Some characteristics of DNA strand scission by macromolecular antitumor
RT   antibiotic C-1027 containing a novel enediyne chromophore.";
RL   Biochemistry 32:5548-5553(1993).
CC   -!- FUNCTION: Binds non-covalently to a chromophore which is the cytotoxic
CC       and mutagenic component of the antibiotic. The chromophore binds to DNA
CC       as a weak intercalator and causes single- and double-strand breaks.
CC   -!- SIMILARITY: Belongs to the neocarzinostatin family. {ECO:0000305}.
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DR   EMBL; D10827; BAA01609.1; -; Genomic_DNA.
DR   PIR; JC1315; YMSMCG.
DR   PDB; 1HZK; NMR; -; A=34-143.
DR   PDB; 1HZL; NMR; -; A=34-143.
DR   PDB; 1J48; X-ray; 1.80 A; A/B=34-143.
DR   PDBsum; 1HZK; -.
DR   PDBsum; 1HZL; -.
DR   PDBsum; 1J48; -.
DR   AlphaFoldDB; Q06110; -.
DR   BMRB; Q06110; -.
DR   SMR; Q06110; -.
DR   DrugBank; DB03933; C-1027 Aromatized chromophore.
DR   EvolutionaryTrace; Q06110; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   InterPro; IPR027273; Neocarzinostatin-like.
DR   InterPro; IPR002186; Neocarzinostatin_fam.
DR   Pfam; PF00960; Neocarzinostat; 1.
DR   PRINTS; PR01885; MACROMOMYCIN.
DR   SUPFAM; SSF49319; SSF49319; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Direct protein sequencing;
KW   Disulfide bond; DNA-binding; Signal.
FT   SIGNAL          1..33
FT                   /evidence="ECO:0000269|PubMed:1368692"
FT   CHAIN           34..143
FT                   /note="Antitumor antibiotic C-1027 apoprotein"
FT                   /id="PRO_0000019459"
FT   DISULFID        69..78
FT   DISULFID        119..124
FT   STRAND          36..40
FT                   /evidence="ECO:0007829|PDB:1J48"
FT   STRAND          43..45
FT                   /evidence="ECO:0007829|PDB:1HZL"
FT   STRAND          50..58
FT                   /evidence="ECO:0007829|PDB:1J48"
FT   STRAND          63..72
FT                   /evidence="ECO:0007829|PDB:1J48"
FT   STRAND          75..78
FT                   /evidence="ECO:0007829|PDB:1J48"
FT   TURN            80..82
FT                   /evidence="ECO:0007829|PDB:1J48"
FT   STRAND          84..87
FT                   /evidence="ECO:0007829|PDB:1J48"
FT   STRAND          94..99
FT                   /evidence="ECO:0007829|PDB:1J48"
FT   STRAND          102..107
FT                   /evidence="ECO:0007829|PDB:1J48"
FT   STRAND          109..111
FT                   /evidence="ECO:0007829|PDB:1HZK"
FT   STRAND          113..118
FT                   /evidence="ECO:0007829|PDB:1J48"
FT   TURN            119..121
FT                   /evidence="ECO:0007829|PDB:1J48"
FT   STRAND          125..130
FT                   /evidence="ECO:0007829|PDB:1J48"
FT   STRAND          133..139
FT                   /evidence="ECO:0007829|PDB:1J48"
SQ   SEQUENCE   143 AA;  13873 MW;  8CA3E57FA5DA842A CRC64;
     MSLRHMSRRA SRFGVVAVAS IGLAAAAQSV AFAAPAFSVS PASGLSDGQS VSVSVSGAAA
     GETYYIAQCA PVGGQDACNP ATATSFTTDA SGAASFSFVV RKSYTGSTPE GTPVGSVDCA
     TAACNLGAGN SGLDLGHVAL TFG