CAGE1_MOUSE
ID CAGE1_MOUSE Reviewed; 849 AA.
AC Q5IR70; A6X913; Q640N7;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Cancer-associated gene 1 protein homolog;
DE Short=CAGE-1;
DE AltName: Full=Cancer/testis antigen 3 homolog;
DE Short=CT3 homolog;
GN Name=Cage1; Synonyms=Ctag3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6N; TISSUE=Testis;
RX PubMed=15819420; DOI=10.1016/j.ejcb.2004.11.003;
RA Alsheimer M., Drewes T., Schutz W., Benavente R.;
RT "The cancer/testis antigen CAGE-1 is a component of the acrosome of
RT spermatids and spermatozoa.";
RL Eur. J. Cell Biol. 84:445-452(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 385-390, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=OF1; TISSUE=Hippocampus;
RA Lubec G., Sunyer B., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-500 (ISOFORM 2), AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5IR70-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5IR70-2; Sequence=VSP_023906, VSP_023907, VSP_023908;
CC -!- TISSUE SPECIFICITY: Expressed in spermatids and spermatozoa. Localized
CC to the acrosomal matrix and acrosomal granule. Predominantly expressed
CC during postmeiotic stages of spermatogenesis.
CC {ECO:0000269|PubMed:15819420}.
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DR EMBL; AY695827; AAV98203.1; -; mRNA.
DR EMBL; CT010477; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC082568; AAH82568.1; -; mRNA.
DR CCDS; CCDS26460.1; -. [Q5IR70-1]
DR RefSeq; NP_082000.2; NM_027724.2. [Q5IR70-1]
DR RefSeq; XP_006516839.1; XM_006516776.3. [Q5IR70-1]
DR AlphaFoldDB; Q5IR70; -.
DR SMR; Q5IR70; -.
DR STRING; 10090.ENSMUSP00000105862; -.
DR iPTMnet; Q5IR70; -.
DR PhosphoSitePlus; Q5IR70; -.
DR PaxDb; Q5IR70; -.
DR PRIDE; Q5IR70; -.
DR ProteomicsDB; 281753; -. [Q5IR70-1]
DR ProteomicsDB; 281754; -. [Q5IR70-2]
DR Antibodypedia; 24634; 150 antibodies from 26 providers.
DR DNASU; 71213; -.
DR Ensembl; ENSMUST00000074969; ENSMUSP00000074499; ENSMUSG00000044566. [Q5IR70-1]
DR Ensembl; ENSMUST00000089840; ENSMUSP00000087278; ENSMUSG00000044566. [Q5IR70-2]
DR Ensembl; ENSMUST00000110233; ENSMUSP00000105862; ENSMUSG00000044566. [Q5IR70-1]
DR GeneID; 71213; -.
DR KEGG; mmu:71213; -.
DR UCSC; uc007qde.2; mouse. [Q5IR70-1]
DR UCSC; uc007qdf.1; mouse. [Q5IR70-2]
DR CTD; 285782; -.
DR MGI; MGI:1918463; Cage1.
DR VEuPathDB; HostDB:ENSMUSG00000044566; -.
DR eggNOG; KOG3650; Eukaryota.
DR GeneTree; ENSGT00390000001805; -.
DR HOGENOM; CLU_020636_0_0_1; -.
DR InParanoid; Q5IR70; -.
DR OMA; SKESHCN; -.
DR OrthoDB; 332349at2759; -.
DR PhylomeDB; Q5IR70; -.
DR TreeFam; TF337775; -.
DR BioGRID-ORCS; 71213; 2 hits in 75 CRISPR screens.
DR ChiTaRS; Cage1; mouse.
DR PRO; PR:Q5IR70; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q5IR70; protein.
DR Bgee; ENSMUSG00000044566; Expressed in spermatid and 79 other tissues.
DR ExpressionAtlas; Q5IR70; baseline and differential.
DR Genevisible; Q5IR70; MM.
DR InterPro; IPR029381; CAGE1.
DR Pfam; PF15066; CAGE1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Direct protein sequencing;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..849
FT /note="Cancer-associated gene 1 protein homolog"
FT /id="PRO_0000280752"
FT REGION 125..160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 241..268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 309..568
FT /evidence="ECO:0000255"
FT COMPBIAS 136..152
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..106
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023906"
FT VAR_SEQ 592..621
FT /note="ETKGTHCNLFLNRSSCKENLELQPLKKTSP -> VSMQTLNSNTIPRVSPSL
FT VMDAQTGHSECS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023907"
FT VAR_SEQ 622..849
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023908"
FT CONFLICT 45
FT /note="G -> R (in Ref. 1; AAV98203)"
FT /evidence="ECO:0000305"
FT CONFLICT 287
FT /note="S -> G (in Ref. 1; AAV98203)"
FT /evidence="ECO:0000305"
FT MOD_RES Q5IR70-2:500
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 849 AA; 97035 MW; C76EB50EC89BC23A CRC64;
MSESETINVN GPSQDFSYSD SPFCMEASFS SSDLLQSETK NVKRGNESTH TFSEDIYSTE
GSLLGDINLG NYPESEQNQP ANTRLSSLRQ FEPICKFHWI EAFNDEMTVE DLRGAFSYSE
KPELPSQVYN DAADGSEKPD PFKEESSVES SISENKDELV PAPVRKSPRS LCLNYYRGEA
QPLTEAPFVR SAVVDVGLNI SQPQSFLDKE NVCKNGDNSS DRENCFEQLD LRAIYKAEEP
EVSSKEVQNS GEISELSVSH QEEVTEDGVD SLAITSPWSP AGIFKGSGPQ DNSLRPDREV
SCEGLEPLEE DMALNEALQK LKQTNKKQEL QIQDLHGKNL NLENRVQELQ TKVTKQHVLV
DIINKLKVNI EELINDKYNV ILEKNDINKK LQDLQEASAH TKKHLQESKK DKESLQLQVK
KIKVHYVRLQ ERYIAEIQQK NRSASQCLEI EKTLSKKDEE LQRLQRHKGE LEKATSSALD
LLKREKEIRE QEFLSFQEEF QRREKESLKE RRKLKSRVEK LVAQVKSLLF TCESERAQTM
ALQRQVEELK LENLELRQLA AKREAQACTP SFEITQSKEQ LEEAVEPDIT QETKGTHCNL
FLNRSSCKEN LELQPLKKTS PLASGIHSLL ALRIGLLTCQ DLATPDAELC QESKKANDIM
LQRLKDCQLK KKDLDKELLK HKNRIATLKE LIASEKALQA HTIEITDFDA DEVKSARDAP
VLLAVKLDKY HSLNEELDVL ITKLGGLLES KEDHYSRLIE ENDKYRRHVG SLINKVTSYE
EIIKCADQRL EISHSQIAHL EERNRHLEDL IRMPREKAKR PRPRLDNHPK SLTLISHLEG
HHKECSISL
