ID   CAGE_HELPJ              Reviewed;         983 AA.
AC   Q9ZLT4;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Type IV secretion system protein CagE {ECO:0000250|UniProtKB:Q48252};
DE            EC=7.4.2.8 {ECO:0000250|UniProtKB:Q48252};
DE   AltName: Full=CAG pathogenicity island protein 23;
DE   AltName: Full=Protein PicB;
GN   Name=cagE; Synonyms=cag23, picB; OrderedLocusNames=jhp_0492;
OS   Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Helicobacteraceae; Helicobacter.
OX   NCBI_TaxID=85963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=J99 / ATCC 700824;
RX   PubMed=9923682; DOI=10.1038/16495;
RA   Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C.,
RA   Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G., Tummino P.J.,
RA   Caruso A., Uria-Nickelsen M., Mills D.M., Ives C., Gibson R., Merberg D.,
RA   Mills S.D., Jiang Q., Taylor D.E., Vovis G.F., Trust T.J.;
RT   "Genomic sequence comparison of two unrelated isolates of the human gastric
RT   pathogen Helicobacter pylori.";
RL   Nature 397:176-180(1999).
CC   -!- FUNCTION: ATPase component of the type IV secretion system Cag (Cag-
CC       T4SS). Acts as a molecular motor to provide the energy that is required
CC       for the export of proteins. Required for CagA translocation and
CC       induction of IL-8 in host gastric epithelial cells. Plays a key role in
CC       Cag-T4SS pilus biogenesis, especially in the localization and
CC       stabilization of the pilus-associated components CagI, CagL and the
CC       surface protein CagH. Is also critical for assembly of the entire
CC       cytoplasmic portion of the Cag inner membrane complex (IMC).
CC       {ECO:0000250|UniProtKB:Q48252}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + cellular proteinSide 1 = ADP + phosphate +
CC         cellular proteinSide 2.; EC=7.4.2.8;
CC         Evidence={ECO:0000250|UniProtKB:Q48252};
CC   -!- SUBUNIT: Component of the Cag type IV secretion system, which is
CC       composed of a wheel-shaped outer membrane complex (OMC) and an inner
CC       membrane complex (IMC) (By similarity). Interacts with CagV and CagBeta
CC       (By similarity). {ECO:0000250|UniProtKB:Q48252}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:Q48252}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q48252}; Cytoplasmic side
CC       {ECO:0000250|UniProtKB:Q48252}.
CC   -!- SIMILARITY: Belongs to the TrbE/VirB4 family. {ECO:0000305}.
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DR   EMBL; AE001439; AAD06065.1; -; Genomic_DNA.
DR   PIR; F71926; F71926.
DR   RefSeq; WP_000496009.1; NZ_CP011330.1.
DR   AlphaFoldDB; Q9ZLT4; -.
DR   SMR; Q9ZLT4; -.
DR   STRING; 85963.jhp_0492; -.
DR   PRIDE; Q9ZLT4; -.
DR   DNASU; 888976; -.
DR   EnsemblBacteria; AAD06065; AAD06065; jhp_0492.
DR   KEGG; hpj:jhp_0492; -.
DR   PATRIC; fig|85963.30.peg.506; -.
DR   eggNOG; COG3451; Bacteria.
DR   OMA; MFMDEFW; -.
DR   Proteomes; UP000000804; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0008564; F:protein-exporting ATPase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR023298; ATPase_P-typ_TM_dom_sf.
DR   InterPro; IPR004346; CagE_TrbE_VirB.
DR   InterPro; IPR018145; CagE_TrbE_VirB_cntrl_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR043964; P-loop_TraG.
DR   Pfam; PF03135; CagE_TrbE_VirB; 1.
DR   Pfam; PF19044; P-loop_TraG; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF81665; SSF81665; 1.
DR   TIGRFAMs; TIGR00929; VirB4_CagE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW   Nucleotide-binding; Translocase; Virulence.
FT   CHAIN           1..983
FT                   /note="Type IV secretion system protein CagE"
FT                   /id="PRO_0000089280"
FT   BINDING         597..604
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   983 AA;  112006 MW;  B35735511D6C9FD2 CRC64;
     MFVASKQADE QKKLVIEQEV QKRQFQKIEE LKADMQKGVN PFFKVLFDGG NRLFGFPETF
     IYSSIFILFV TIVLSVILFQ AYEPVLIVAI VIVLVALGFK KDYRLYQRME RAMKFKKPFL
     FKGVKNKAFM SIFSMKPSKE MANDIHLNPN REDRLVSAAN SYLANNYECF LDDGVILTNN
     YSLLGTIKLG GIDFLTTSKK DLIELHASIY SVFRNFVTPE FKFYFHTVKK KIVIDETNRD
     YGLIFSNDFM RAYNEKQKRE SFYDISFYLT IEQDLLDTLN EPVMNKKHFA DNNFEEFQRI
     IRAKLENFKD RIELIEELLS KYHPTRLKEY TKDGIIYSKQ CEFYNFLVGM NEAPFICNRK
     DLYLKEKMHG GVKEVYFANK HGKILNDDLS EKYFSAIEIS EYAPKSQSDL FDKINALDSE
     FIFMHAYSPK NSQVLKDKLA FTSRRIIISG GSKEQGMTLG CLSELVGNGD ITLGSYGNSL
     VLFADSFEKM KQSVKECVSS LNAKGFLANA ATFSMENYFF AKHCSFITLP FIFDVTSNNF
     ADFIAMRAMS FDGKEDNNAW GNSVMTLKSE INSPFYLNFH MPTDFGSASA GHTLILGSTG
     SGKTVFMSMT LNAMGQFAYN FPANISKDKQ KLTMVYMDKD YGAYGNIVAM GGEYVKIELG
     TDTGLNPFAW AACVQKTNAT MEQKQTAISV VKELVKNLAT KSDEKDENGN SISFSLADSN
     TLAAAVTNLI TGDMNLDYPI TQLINAFGKD HNDPNGLVAR LAPFCKSTNG EFQWLFDNKA
     TDRLDFSKTI IGVDGSSFLD NNDVSPFICF YLFARIQEAM DGRRFVLDID EAWKYLGDPK
     VAYFVRDMLK TARKRNAIVR LATQSITDLL ACPIADTIRE QCPTKIFLRN DGGNLSDYQR
     LANVTEKEFE IITKGLDRKI LYKQDGSPSV IASFNLRGIP KEYLKILSTD TVFVKEIDKI
     IQNHSIIDKY QALRQMYQQI KEY