UXT1_ARATH
ID UXT1_ARATH Reviewed; 342 AA.
AC F4IHS9; B3LFB1;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 71.
DE RecName: Full=UDP-xylose transporter 1 {ECO:0000312|EMBL:AKA88212.1};
GN Name=UXT1 {ECO:0000312|EMBL:AKA88212.1};
GN OrderedLocusNames=At2g28315 {ECO:0000312|Araport:AT2G28315};
GN ORFNames=T1B3.18 {ECO:0000312|EMBL:AC006283};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY,
RP SUBCELLULAR LOCATION, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=25804536; DOI=10.1105/tpc.114.133827;
RA Ebert B., Rautengarten C., Guo X., Xiong G., Stonebloom S.,
RA Smith-Moritz A.M., Herter T., Chan L.J., Adams P.D., Petzold C.J.,
RA Pauly M., Willats W.G., Heazlewood J.L., Scheller H.V.;
RT "Identification and characterization of a Golgi-localized UDP-xylose
RT transporter family from Arabidopsis.";
RL Plant Cell 27:1218-1227(2015).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 103-342.
RC STRAIN=cv. Columbia;
RA de los Reyes C., Quan R., Chen H., Bautista V., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=25053812; DOI=10.1073/pnas.1406073111;
RA Rautengarten C., Ebert B., Moreno I., Temple H., Herter T., Link B.,
RA Donas-Cofre D., Moreno A., Saez-Aguayo S., Blanco F., Mortimer J.C.,
RA Schultink A., Reiter W.D., Dupree P., Pauly M., Heazlewood J.L.,
RA Scheller H.V., Orellana A.;
RT "The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter
RT family of Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11563-11568(2014).
CC -!- FUNCTION: Nucleotide-sugar transporter that transports UDP-xylose and
CC UMP in a strict counter-exchange mode. {ECO:0000269|PubMed:25804536}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=39 uM for UDP-Xylose {ECO:0000269|PubMed:25804536};
CC Vmax=16 nmol/sec/mg enzyme toward UDP-Xylose
CC {ECO:0000269|PubMed:25804536};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:25804536}; Multi-pass membrane protein
CC {ECO:0000255}. Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:25804536}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:25804536}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype. Slight reduction of xylose
CC content in the cell wall composition. {ECO:0000269|PubMed:25804536}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. TPT (TC 2.A.7.9)
CC subfamily. {ECO:0000305}.
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DR EMBL; KP872766; AKA88212.1; -; mRNA.
DR EMBL; AC006283; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002685; AEC08106.1; -; Genomic_DNA.
DR EMBL; BT033099; ACF09415.1; -; mRNA.
DR RefSeq; NP_850120.3; NM_179789.4.
DR AlphaFoldDB; F4IHS9; -.
DR SMR; F4IHS9; -.
DR IntAct; F4IHS9; 70.
DR STRING; 3702.AT2G28315.1; -.
DR PaxDb; F4IHS9; -.
DR PRIDE; F4IHS9; -.
DR ProteomicsDB; 243245; -.
DR EnsemblPlants; AT2G28315.1; AT2G28315.1; AT2G28315.
DR GeneID; 817378; -.
DR Gramene; AT2G28315.1; AT2G28315.1; AT2G28315.
DR KEGG; ath:AT2G28315; -.
DR Araport; AT2G28315; -.
DR TAIR; locus:504955965; AT2G28315.
DR eggNOG; KOG1441; Eukaryota.
DR HOGENOM; CLU_048347_0_1_1; -.
DR InParanoid; F4IHS9; -.
DR OrthoDB; 938634at2759; -.
DR PhylomeDB; F4IHS9; -.
DR PRO; PR:F4IHS9; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IHS9; baseline and differential.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IDA:UniProtKB.
DR GO; GO:0005338; F:nucleotide-sugar transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005464; F:UDP-xylose transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0009834; P:plant-type secondary cell wall biogenesis; IGI:TAIR.
DR GO; GO:1900030; P:regulation of pectin biosynthetic process; IGI:TAIR.
DR GO; GO:0015790; P:UDP-xylose transmembrane transport; IDA:TAIR.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR Pfam; PF03151; TPT; 1.
PE 1: Evidence at protein level;
KW Antiport; Endoplasmic reticulum; Golgi apparatus; Membrane;
KW Reference proteome; Sugar transport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..342
FT /note="UDP-xylose transporter 1"
FT /id="PRO_0000439525"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 184..204
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 305..342
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 321..342
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 342 AA; 37444 MW; 670493B6FA17BABD CRC64;
MGEMKSMQMG VIGALFLSVA SSVSIVICNK ALMTNLGFPF ATTLTSWHLM VTYCTLHVAY
KLNFFENKPI DMRTVVLFGL LNGISIGLLN LSLGFNSIGF YQMTKLAIIP FTVLLETLFL
NKKFSQKIKF SLFLLLVGVG IASITDLQLN FVGSVLSLLA IATTCVGQIL TNTIQKRLNV
TSTQLLYQSA PFQAAILFVS GPFVDKYLTS LNVFSFHYSP IVVGFITLSC LIAVSVNFST
FLVIGKTSPV TYQVLGHLKT CLVLAFGYTL LHDPFTPRNI AGILIAVLGM LLYSYFCSVA
SKSKQASSDS TFLGKDRDTT PLLGQENENH HEAKKLDKHS PV
