UXT2_ARATH
ID UXT2_ARATH Reviewed; 353 AA.
AC Q8GUJ1; O04347;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=UDP-xylose transporter 2 {ECO:0000312|EMBL:AKA88213.1};
GN Name=UXT2 {ECO:0000312|EMBL:AKA88213.1};
GN OrderedLocusNames=At2g30460 {ECO:0000312|Araport:AT2G30460};
GN ORFNames=T6B20.18 {ECO:0000312|EMBL:AAB63090.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY,
RP SUBCELLULAR LOCATION, FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=cv. Columbia;
RX PubMed=25804536; DOI=10.1105/tpc.114.133827;
RA Ebert B., Rautengarten C., Guo X., Xiong G., Stonebloom S.,
RA Smith-Moritz A.M., Herter T., Chan L.J., Adams P.D., Petzold C.J.,
RA Pauly M., Willats W.G., Heazlewood J.L., Scheller H.V.;
RT "Identification and characterization of a Golgi-localized UDP-xylose
RT transporter family from Arabidopsis.";
RL Plant Cell 27:1218-1227(2015).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=25053812; DOI=10.1073/pnas.1406073111;
RA Rautengarten C., Ebert B., Moreno I., Temple H., Herter T., Link B.,
RA Donas-Cofre D., Moreno A., Saez-Aguayo S., Blanco F., Mortimer J.C.,
RA Schultink A., Reiter W.D., Dupree P., Pauly M., Heazlewood J.L.,
RA Scheller H.V., Orellana A.;
RT "The Golgi localized bifunctional UDP-rhamnose/UDP-galactose transporter
RT family of Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 111:11563-11568(2014).
CC -!- FUNCTION: Nucleotide-sugar transporter that transports UDP-xylose and
CC UMP in a strict counter-exchange mode. {ECO:0000269|PubMed:25804536}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=40 uM for UDP-Xylose {ECO:0000269|PubMed:25804536};
CC Vmax=13 nmol/sec/mg enzyme toward UDP-Xylose
CC {ECO:0000269|PubMed:25804536};
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:25804536}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:25804536}.
CC -!- SIMILARITY: Belongs to the TPT transporter family. TPT (TC 2.A.7.9)
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB63090.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; KP872767; AKA88213.1; -; mRNA.
DR EMBL; U93215; AAB63090.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08390.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08391.1; -; Genomic_DNA.
DR EMBL; BT002471; AAO00831.1; -; mRNA.
DR EMBL; BT008742; AAP42755.1; -; mRNA.
DR EMBL; AK176166; BAD43929.1; -; mRNA.
DR EMBL; AK176178; BAD43941.1; -; mRNA.
DR EMBL; AK176274; BAD44037.1; -; mRNA.
DR EMBL; AK221221; BAD93797.1; -; mRNA.
DR PIR; F84708; F84708.
DR RefSeq; NP_001154542.1; NM_001161070.2.
DR RefSeq; NP_180604.4; NM_128598.5.
DR AlphaFoldDB; Q8GUJ1; -.
DR SMR; Q8GUJ1; -.
DR STRING; 3702.AT2G30460.2; -.
DR PaxDb; Q8GUJ1; -.
DR PRIDE; Q8GUJ1; -.
DR EnsemblPlants; AT2G30460.1; AT2G30460.1; AT2G30460.
DR EnsemblPlants; AT2G30460.2; AT2G30460.2; AT2G30460.
DR GeneID; 817596; -.
DR Gramene; AT2G30460.1; AT2G30460.1; AT2G30460.
DR Gramene; AT2G30460.2; AT2G30460.2; AT2G30460.
DR KEGG; ath:AT2G30460; -.
DR Araport; AT2G30460; -.
DR TAIR; locus:2064316; AT2G30460.
DR eggNOG; KOG1441; Eukaryota.
DR HOGENOM; CLU_048347_0_1_1; -.
DR InParanoid; Q8GUJ1; -.
DR OMA; VARWMRF; -.
DR OrthoDB; 938634at2759; -.
DR PhylomeDB; Q8GUJ1; -.
DR PRO; PR:Q8GUJ1; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8GUJ1; baseline and differential.
DR GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015297; F:antiporter activity; IDA:UniProtKB.
DR GO; GO:0005338; F:nucleotide-sugar transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005464; F:UDP-xylose transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0015790; P:UDP-xylose transmembrane transport; IDA:TAIR.
DR InterPro; IPR004853; Sugar_P_trans_dom.
DR Pfam; PF03151; TPT; 1.
PE 1: Evidence at protein level;
KW Antiport; Golgi apparatus; Membrane; Phosphoprotein; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..353
FT /note="UDP-xylose transporter 2"
FT /id="PRO_0000439526"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 308..353
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 329..353
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 334
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9FDZ5"
SQ SEQUENCE 353 AA; 38830 MW; 11237B381E04421C CRC64;
MSDAQKFQLG TIGALSLSVV SSVSIVICNK ALISTLGFTF ATTLTSWHLL VTFCSLHVAL
WMKFFEHKPF DPRAVLGFGV LNGISIGLLN LSLGFNSVGF YQMTKLAIIP CTVVLETIFF
RKMFSRKIQF SLVILLLGVG IATVTDLQLN MLGSVLSLLA VITTCVAQIM TNTIQKKYKV
SSTQLLYQSC PYQAITLFVT GPFLDGLLTN QNVFAFKYTS QVVFFIVLSC LISVSVNFST
FLVIGKTSPV TYQVLGHLKT CLVLAFGYLL LKDAFSWRNI LGILVAVIGM VLYSYYCTLE
TQQKATETST QLPQMDENEK DPLVSAENGS GLISDNGVQK QDPVWNSNKD FQA
