ID   UXUA1_AGRFC             Reviewed;         402 AA.
AC   Q8UAW0;
DT   11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Mannonate dehydratase 1;
DE            EC=4.2.1.8;
DE   AltName: Full=D-mannonate hydro-lyase 1;
GN   Name=uxuA1; OrderedLocusNames=Atu3257; ORFNames=AGR_L_3119;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: Catalyzes the dehydration of D-mannonate. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O;
CC         Xref=Rhea:RHEA:20097, ChEBI:CHEBI:15377, ChEBI:CHEBI:17767,
CC         ChEBI:CHEBI:57990; EC=4.2.1.8;
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carbohydrate metabolism; pentose and glucuronate
CC       interconversion.
CC   -!- SIMILARITY: Belongs to the mannonate dehydratase family. {ECO:0000305}.
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DR   EMBL; AE007870; AAK90133.1; -; Genomic_DNA.
DR   PIR; AC2957; AC2957.
DR   PIR; C98326; C98326.
DR   RefSeq; NP_357348.1; NC_003063.2.
DR   RefSeq; WP_010972887.1; NC_003063.2.
DR   AlphaFoldDB; Q8UAW0; -.
DR   SMR; Q8UAW0; -.
DR   STRING; 176299.Atu3257; -.
DR   EnsemblBacteria; AAK90133; AAK90133; Atu3257.
DR   GeneID; 66223568; -.
DR   KEGG; atu:Atu3257; -.
DR   PATRIC; fig|176299.10.peg.3099; -.
DR   eggNOG; COG1312; Bacteria.
DR   HOGENOM; CLU_058621_2_0_5; -.
DR   OMA; GIVWALH; -.
DR   PhylomeDB; Q8UAW0; -.
DR   BioCyc; AGRO:ATU3257-MON; -.
DR   UniPathway; UPA00246; -.
DR   Proteomes; UP000000813; Chromosome linear.
DR   GO; GO:0008927; F:mannonate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006064; P:glucuronate catabolic process; IEA:InterPro.
DR   HAMAP; MF_00106; UxuA; 1.
DR   InterPro; IPR004628; Man_deHydtase.
DR   InterPro; IPR036237; Xyl_isomerase-like_sf.
DR   PANTHER; PTHR30387; PTHR30387; 1.
DR   Pfam; PF03786; UxuA; 1.
DR   PIRSF; PIRSF016049; Man_dehyd; 1.
DR   SUPFAM; SSF51658; SSF51658; 1.
DR   TIGRFAMs; TIGR00695; uxuA; 1.
PE   3: Inferred from homology;
KW   Iron; Lyase; Manganese; Reference proteome.
FT   CHAIN           1..402
FT                   /note="Mannonate dehydratase 1"
FT                   /id="PRO_0000170658"
SQ   SEQUENCE   402 AA;  44690 MW;  4C22D27792F37705 CRC64;
     MRHTWRWFGP VDKVTVQDAA QAGAEGIVSA LHHITTGDVW PVDEITKRHE AIKAGGLYWD
     VVESVPVSED IKTQTGDWKN HIANWQETLR RLSASGIRTV CYNFMPVLDW TRTDLRWETR
     HGARAMRFDL TDFAAFDIHI LKRPDAKADY PDWLLEEAAK RFAEMPDTKI AALGRNIGAG
     LPGSADGYTL AQLLEKLRSY HGINRGRLQQ NLIDFLSEVT PVAEEVGINI CAHGDDPPWP
     LLGLPRILST EADYAHMLSQ VDSRANGVTL CTGSLGARAD NDLPFIAGRF ADRIHFVHLR
     NVTRDTDTVP CSFFEDEHLE GGTDMVAVIA ALITEEARRR AEGRDDHTIP MRPDHGQEIL
     DDLTRGAQPG YPAIGRLKGL AELRGIERTL SHGRFGLATG KG