ID   UXUA2_AGRFC             Reviewed;         397 AA.
AC   Q8UA46;
DT   11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Mannonate dehydratase 2;
DE            EC=4.2.1.8;
DE   AltName: Full=D-mannonate hydro-lyase 2;
GN   Name=uxuA2; OrderedLocusNames=Atu3529; ORFNames=AGR_L_2605;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: Catalyzes the dehydration of D-mannonate. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O;
CC         Xref=Rhea:RHEA:20097, ChEBI:CHEBI:15377, ChEBI:CHEBI:17767,
CC         ChEBI:CHEBI:57990; EC=4.2.1.8;
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carbohydrate metabolism; pentose and glucuronate
CC       interconversion.
CC   -!- SIMILARITY: Belongs to the mannonate dehydratase family. {ECO:0000305}.
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DR   EMBL; AE007870; AAK89868.1; -; Genomic_DNA.
DR   PIR; AG2990; AG2990.
DR   PIR; B98293; B98293.
DR   RefSeq; NP_357083.1; NC_003063.2.
DR   RefSeq; WP_010973116.1; NC_003063.2.
DR   AlphaFoldDB; Q8UA46; -.
DR   SMR; Q8UA46; -.
DR   STRING; 176299.Atu3529; -.
DR   EnsemblBacteria; AAK89868; AAK89868; Atu3529.
DR   GeneID; 66223827; -.
DR   KEGG; atu:Atu3529; -.
DR   PATRIC; fig|176299.10.peg.3370; -.
DR   eggNOG; COG1312; Bacteria.
DR   HOGENOM; CLU_058621_2_0_5; -.
DR   OMA; AIVKAYH; -.
DR   PhylomeDB; Q8UA46; -.
DR   BioCyc; AGRO:ATU3529-MON; -.
DR   UniPathway; UPA00246; -.
DR   Proteomes; UP000000813; Chromosome linear.
DR   GO; GO:0008927; F:mannonate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006064; P:glucuronate catabolic process; IEA:InterPro.
DR   HAMAP; MF_00106; UxuA; 1.
DR   InterPro; IPR004628; Man_deHydtase.
DR   InterPro; IPR036237; Xyl_isomerase-like_sf.
DR   PANTHER; PTHR30387; PTHR30387; 1.
DR   Pfam; PF03786; UxuA; 1.
DR   PIRSF; PIRSF016049; Man_dehyd; 1.
DR   SUPFAM; SSF51658; SSF51658; 1.
DR   TIGRFAMs; TIGR00695; uxuA; 1.
PE   3: Inferred from homology;
KW   Iron; Lyase; Manganese; Reference proteome.
FT   CHAIN           1..397
FT                   /note="Mannonate dehydratase 2"
FT                   /id="PRO_0000170659"
SQ   SEQUENCE   397 AA;  43649 MW;  4A2278A2032E53E9 CRC64;
     MKETWRWFGE SDPITLEHVR QTGASGVVTA LHQIPDGTAW PAEEIAKRKA MIEAAGLEWS
     VCESIPMEQS IKRGDADAPK AIARWKDTLS RLGRAGVPVV CYNFMPVVDW TRTNLRWQAR
     NTGLALRFEM ADFVAYDVFI LKRIRAAENY DPALVARAEE RFAQMSEDEQ SLLERNIIAG
     LPGGALVQTR QSIAALIASF DGIDSATMQG NLLAFLKEVV PVAEEVGVHL GIHPDDPPFS
     LFGLPRVVST PADIRAILSA VESPNNGITL CTGSYGARSD NDLVAMAKEF ASRVNFAHLR
     NVTVEADGSF FEDDHLDGGA DMIGVIEALL REERSTAKAG RRTNIPMRPD HGHLLGDDIT
     KKTNPGYSYI GRMKGLGELR GVIRTIERQL RREEAAA