ID   UXUA2_BACLD             Reviewed;         356 AA.
AC   Q65IW0; Q62UB8;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Mannonate dehydratase 2 {ECO:0000255|HAMAP-Rule:MF_00106};
DE            EC=4.2.1.8 {ECO:0000255|HAMAP-Rule:MF_00106};
DE   AltName: Full=D-mannonate hydro-lyase 2 {ECO:0000255|HAMAP-Rule:MF_00106};
GN   Name=uxuA2 {ECO:0000255|HAMAP-Rule:MF_00106};
GN   OrderedLocusNames=BLi02116, BL00281;
OS   Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 /
OS   NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=279010;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375
RC   / NCTC 10341 / NRRL NRS-1264 / Gibson 46;
RX   PubMed=15383718; DOI=10.1159/000079829;
RA   Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P.,
RA   Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.;
RT   "The complete genome sequence of Bacillus licheniformis DSM13, an organism
RT   with great industrial potential.";
RL   J. Mol. Microbiol. Biotechnol. 7:204-211(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375
RC   / NCTC 10341 / NRRL NRS-1264 / Gibson 46;
RX   PubMed=15461803; DOI=10.1186/gb-2004-5-10-r77;
RA   Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J.,
RA   Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B.,
RA   Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A.,
RA   Bolotin A., Lapidus A., Galleron N., Ehrlich S.D., Berka R.M.;
RT   "Complete genome sequence of the industrial bacterium Bacillus
RT   licheniformis and comparisons with closely related Bacillus species.";
RL   Genome Biol. 5:R77.1-R77.12(2004).
CC   -!- FUNCTION: Catalyzes the dehydration of D-mannonate. {ECO:0000255|HAMAP-
CC       Rule:MF_00106}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O;
CC         Xref=Rhea:RHEA:20097, ChEBI:CHEBI:15377, ChEBI:CHEBI:17767,
CC         ChEBI:CHEBI:57990; EC=4.2.1.8; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00106};
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00106};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00106};
CC   -!- PATHWAY: Carbohydrate metabolism; pentose and glucuronate
CC       interconversion. {ECO:0000255|HAMAP-Rule:MF_00106}.
CC   -!- SIMILARITY: Belongs to the mannonate dehydratase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00106}.
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DR   EMBL; AE017333; AAU41004.1; -; Genomic_DNA.
DR   EMBL; CP000002; AAU23641.2; -; Genomic_DNA.
DR   RefSeq; WP_003182399.1; NC_006322.1.
DR   AlphaFoldDB; Q65IW0; -.
DR   SMR; Q65IW0; -.
DR   STRING; 279010.BL00281; -.
DR   EnsemblBacteria; AAU23641; AAU23641; BL00281.
DR   GeneID; 66215881; -.
DR   KEGG; bld:BLi02116; -.
DR   KEGG; bli:BL00281; -.
DR   eggNOG; COG1312; Bacteria.
DR   HOGENOM; CLU_058621_1_0_9; -.
DR   OMA; AIVKAYH; -.
DR   OrthoDB; 907794at2; -.
DR   BioCyc; BLIC279010:BLI_RS10465-MON; -.
DR   UniPathway; UPA00246; -.
DR   Proteomes; UP000000606; Chromosome.
DR   GO; GO:0008927; F:mannonate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006064; P:glucuronate catabolic process; IEA:InterPro.
DR   HAMAP; MF_00106; UxuA; 1.
DR   InterPro; IPR004628; Man_deHydtase.
DR   InterPro; IPR036237; Xyl_isomerase-like_sf.
DR   PANTHER; PTHR30387; PTHR30387; 1.
DR   Pfam; PF03786; UxuA; 1.
DR   PIRSF; PIRSF016049; Man_dehyd; 1.
DR   SUPFAM; SSF51658; SSF51658; 1.
DR   TIGRFAMs; TIGR00695; uxuA; 2.
PE   3: Inferred from homology;
KW   Iron; Lyase; Manganese; Reference proteome.
FT   CHAIN           1..356
FT                   /note="Mannonate dehydratase 2"
FT                   /id="PRO_0000231048"
SQ   SEQUENCE   356 AA;  40830 MW;  F3293B5FCD5F964D CRC64;
     MQMTFRWYGD SDPVTLEYIR QIPGVTGIVS AIYDIPVGEA WPYEKIVELK EKIENHGLSL
     SVIESVPVHE DIKLGLPTRD QYIENYKTTI RNLAKAGVKI VCYNFMPVFD WTRSSLDYAL
     EDGSTALIYE EEKVRQMNPL HGELKLPGWD TSYEDGQLKN LFQQYQHMTE EDLWENLSYF
     IKAVIPAAEN EQVKMAIHPD DPPWPIFGLP RIITNKENLE RLIHLYDSSF NGLCLCSGSL
     GVKSTNDIPE LIRYFGKKGK VNFVHLRNIK IIGEKSFQES SHRSEDGSLD MYEIVRALRD
     IDFAGPARPD HGRMIWGETG KPGYGLYDRA LGAVYLNGMW ETLTKEKKRI ALECNK