ID   UXUA_ALKCK              Reviewed;         358 AA.
AC   Q5WKD5;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Mannonate dehydratase {ECO:0000255|HAMAP-Rule:MF_00106};
DE            EC=4.2.1.8 {ECO:0000255|HAMAP-Rule:MF_00106};
DE   AltName: Full=D-mannonate hydro-lyase {ECO:0000255|HAMAP-Rule:MF_00106};
GN   Name=uxuA {ECO:0000255|HAMAP-Rule:MF_00106}; OrderedLocusNames=ABC0631;
OS   Alkalihalobacillus clausii (strain KSM-K16) (Bacillus clausii).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus.
OX   NCBI_TaxID=66692;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KSM-K16;
RA   Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y.,
RA   Kawai S., Ito S., Horikoshi K.;
RT   "The complete genome sequence of the alkaliphilic Bacillus clausii KSM-
RT   K16.";
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the dehydration of D-mannonate. {ECO:0000255|HAMAP-
CC       Rule:MF_00106}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O;
CC         Xref=Rhea:RHEA:20097, ChEBI:CHEBI:15377, ChEBI:CHEBI:17767,
CC         ChEBI:CHEBI:57990; EC=4.2.1.8; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00106};
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00106};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00106};
CC   -!- PATHWAY: Carbohydrate metabolism; pentose and glucuronate
CC       interconversion. {ECO:0000255|HAMAP-Rule:MF_00106}.
CC   -!- SIMILARITY: Belongs to the mannonate dehydratase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00106}.
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DR   EMBL; AP006627; BAD63170.1; -; Genomic_DNA.
DR   RefSeq; WP_011245486.1; NC_006582.1.
DR   AlphaFoldDB; Q5WKD5; -.
DR   SMR; Q5WKD5; -.
DR   STRING; 66692.ABC0631; -.
DR   PRIDE; Q5WKD5; -.
DR   EnsemblBacteria; BAD63170; BAD63170; ABC0631.
DR   KEGG; bcl:ABC0631; -.
DR   eggNOG; COG1312; Bacteria.
DR   HOGENOM; CLU_058621_1_0_9; -.
DR   OMA; GIVWALH; -.
DR   OrthoDB; 907794at2; -.
DR   UniPathway; UPA00246; -.
DR   Proteomes; UP000001168; Chromosome.
DR   GO; GO:0008927; F:mannonate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006064; P:glucuronate catabolic process; IEA:InterPro.
DR   HAMAP; MF_00106; UxuA; 1.
DR   InterPro; IPR004628; Man_deHydtase.
DR   InterPro; IPR036237; Xyl_isomerase-like_sf.
DR   PANTHER; PTHR30387; PTHR30387; 1.
DR   Pfam; PF03786; UxuA; 1.
DR   PIRSF; PIRSF016049; Man_dehyd; 1.
DR   SUPFAM; SSF51658; SSF51658; 1.
DR   TIGRFAMs; TIGR00695; uxuA; 1.
PE   3: Inferred from homology;
KW   Iron; Lyase; Manganese; Reference proteome.
FT   CHAIN           1..358
FT                   /note="Mannonate dehydratase"
FT                   /id="PRO_0000170662"
SQ   SEQUENCE   358 AA;  41342 MW;  0CD7E5E203BF6B4A CRC64;
     MRMTFRWYGE NNDSVTLEQI KQIPGVEGLV WALHDKVAGE VWPLEDIMQV KEQADRYGFH
     LDVVESINVH EDIKLGLPTR DAYIENYKES IRNVAKVGAK VICYNFMPVF DWTRTDLFKE
     MEDGSTALFY EKAKVDNMDP RELVRQTTSN AAFTMPGWEP ERLAHIEKSL KAYENVTEDD
     LWEHLQYFLE QVLPVAEEHG IQMAIHPDDP PWSVFGLPRI ITSEAAVERF LQLSNSPAHG
     ITLCSGSLGA NPENDIPKII RRFHDRIPFA HIRNVKIYEN GDFIETSHRS QDGSVNIADV
     VKAYHENGFT GYVRPDHGRH IWNEKCRPGY GLYDRALGIM HLWGLWDAYE LEAKRRQS