ID   UXUA_BACSU              Reviewed;         359 AA.
AC   O34346;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Mannonate dehydratase;
DE            EC=4.2.1.8;
DE   AltName: Full=D-mannonate hydro-lyase;
GN   Name=uxuA; Synonyms=yjmE; OrderedLocusNames=BSU12340;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROBABLE OPERON STRUCTURE.
RC   STRAIN=168;
RX   PubMed=9579062; DOI=10.1099/00221287-144-4-877;
RA   Rivolta C., Soldo B., Lazarevic V., Joris B., Mauel C., Karamata D.;
RT   "A 35.7 kb DNA fragment from the Bacillus subtilis chromosome containing a
RT   putative 12.3 kb operon involved in hexuronate catabolism and a perfectly
RT   symmetrical hypothetical catabolite-responsive element.";
RL   Microbiology 144:877-884(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   PROBABLE OPERON STRUCTURE, AND INDUCTION.
RC   STRAIN=168 / MB24;
RX   PubMed=9882655; DOI=10.1128/jb.181.2.426-433.1999;
RA   Mekjian K.R., Bryan E.M., Beall B.W., Moran C.P. Jr.;
RT   "Regulation of hexuronate utilization in Bacillus subtilis.";
RL   J. Bacteriol. 181:426-433(1999).
CC   -!- FUNCTION: Catalyzes the dehydration of D-mannonate. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O;
CC         Xref=Rhea:RHEA:20097, ChEBI:CHEBI:15377, ChEBI:CHEBI:17767,
CC         ChEBI:CHEBI:57990; EC=4.2.1.8;
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carbohydrate metabolism; pentose and glucuronate
CC       interconversion.
CC   -!- INDUCTION: Induced by galacturonate, repressed by glucose.
CC       {ECO:0000305|PubMed:9882655}.
CC   -!- MISCELLANEOUS: Member of the exu locus which is required for
CC       galacturonate utilization.
CC   -!- SIMILARITY: Belongs to the mannonate dehydratase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF015825; AAC46330.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB13091.1; -; Genomic_DNA.
DR   PIR; G69852; G69852.
DR   RefSeq; NP_389116.1; NC_000964.3.
DR   RefSeq; WP_003244855.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; O34346; -.
DR   SMR; O34346; -.
DR   STRING; 224308.BSU12340; -.
DR   PaxDb; O34346; -.
DR   EnsemblBacteria; CAB13091; CAB13091; BSU_12340.
DR   GeneID; 939409; -.
DR   KEGG; bsu:BSU12340; -.
DR   PATRIC; fig|224308.179.peg.1335; -.
DR   eggNOG; COG1312; Bacteria.
DR   InParanoid; O34346; -.
DR   OMA; GIVWALH; -.
DR   PhylomeDB; O34346; -.
DR   BioCyc; BSUB:BSU12340-MON; -.
DR   UniPathway; UPA00246; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0008198; F:ferrous iron binding; IBA:GO_Central.
DR   GO; GO:0030145; F:manganese ion binding; IBA:GO_Central.
DR   GO; GO:0008927; F:mannonate dehydratase activity; IBA:GO_Central.
DR   GO; GO:0042840; P:D-glucuronate catabolic process; IBA:GO_Central.
DR   HAMAP; MF_00106; UxuA; 1.
DR   InterPro; IPR004628; Man_deHydtase.
DR   InterPro; IPR036237; Xyl_isomerase-like_sf.
DR   PANTHER; PTHR30387; PTHR30387; 1.
DR   Pfam; PF03786; UxuA; 1.
DR   PIRSF; PIRSF016049; Man_dehyd; 1.
DR   SUPFAM; SSF51658; SSF51658; 1.
DR   TIGRFAMs; TIGR00695; uxuA; 1.
PE   2: Evidence at transcript level;
KW   Iron; Lyase; Manganese; Reference proteome.
FT   CHAIN           1..359
FT                   /note="Mannonate dehydratase"
FT                   /id="PRO_0000170664"
SQ   SEQUENCE   359 AA;  41024 MW;  BAEA060E1C278CDF CRC64;
     MNMTFRWYGR GNDTVTLEYV KQIPGVKGIV WALHQKPVGD VWEKEEIRAE TEYIQSYGFH
     AEVVESVNVH EAIKLGNEER GRYIENYKQT IRNLAGFGVK VICYNFMPVF DWTRTDMFRP
     LEDGSTALFF EKAKVESLDP QELIRTVEEA SDMTLPGWEP EKLARIKELF AAYRTVDEEK
     LWDNLSFFLQ EILPVAEAYG VQMAIHPDDP PWPIFGLPRI ITGEASYKKL RAISDSPSNC
     ITLCTGSMGA NPANDMVEIA KTYAGIAPFS HIRNVKIYEN GDFIETSHLT KDGSINIQGV
     MEELHKQDYE GYVRPDHGRH LWGEQCRPGY GLYDRALGIM YLNGLWDAYE AMAKKEVGI