ID   UXUA_CLOAB              Reviewed;         351 AA.
AC   Q97JF4;
DT   06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 102.
DE   RecName: Full=Mannonate dehydratase {ECO:0000255|HAMAP-Rule:MF_00106};
DE            EC=4.2.1.8 {ECO:0000255|HAMAP-Rule:MF_00106};
DE   AltName: Full=D-mannonate hydro-lyase {ECO:0000255|HAMAP-Rule:MF_00106};
GN   Name=uxuA {ECO:0000255|HAMAP-Rule:MF_00106}; OrderedLocusNames=CA_C1332;
OS   Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710
OS   / VKM B-1787).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Clostridium.
OX   NCBI_TaxID=272562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
RX   PubMed=11466286; DOI=10.1128/jb.183.16.4823-4838.2001;
RA   Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R.,
RA   Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F.,
RA   Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V.,
RA   Smith D.R.;
RT   "Genome sequence and comparative analysis of the solvent-producing
RT   bacterium Clostridium acetobutylicum.";
RL   J. Bacteriol. 183:4823-4838(2001).
CC   -!- FUNCTION: Catalyzes the dehydration of D-mannonate. {ECO:0000255|HAMAP-
CC       Rule:MF_00106}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O;
CC         Xref=Rhea:RHEA:20097, ChEBI:CHEBI:15377, ChEBI:CHEBI:17767,
CC         ChEBI:CHEBI:57990; EC=4.2.1.8; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00106};
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00106};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00106};
CC   -!- PATHWAY: Carbohydrate metabolism; pentose and glucuronate
CC       interconversion. {ECO:0000255|HAMAP-Rule:MF_00106}.
CC   -!- SIMILARITY: Belongs to the mannonate dehydratase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00106}.
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DR   EMBL; AE001437; AAK79300.1; -; Genomic_DNA.
DR   PIR; A97064; A97064.
DR   RefSeq; NP_347960.1; NC_003030.1.
DR   RefSeq; WP_010964641.1; NC_003030.1.
DR   AlphaFoldDB; Q97JF4; -.
DR   SMR; Q97JF4; -.
DR   STRING; 272562.CA_C1332; -.
DR   EnsemblBacteria; AAK79300; AAK79300; CA_C1332.
DR   GeneID; 44997837; -.
DR   KEGG; cac:CA_C1332; -.
DR   PATRIC; fig|272562.8.peg.1537; -.
DR   eggNOG; COG1312; Bacteria.
DR   HOGENOM; CLU_058621_1_0_9; -.
DR   OMA; AIVKAYH; -.
DR   OrthoDB; 907794at2; -.
DR   UniPathway; UPA00246; -.
DR   Proteomes; UP000000814; Chromosome.
DR   GO; GO:0008927; F:mannonate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006064; P:glucuronate catabolic process; IEA:InterPro.
DR   HAMAP; MF_00106; UxuA; 1.
DR   InterPro; IPR004628; Man_deHydtase.
DR   InterPro; IPR036237; Xyl_isomerase-like_sf.
DR   PANTHER; PTHR30387; PTHR30387; 1.
DR   Pfam; PF03786; UxuA; 1.
DR   PIRSF; PIRSF016049; Man_dehyd; 1.
DR   SUPFAM; SSF51658; SSF51658; 1.
DR   TIGRFAMs; TIGR00695; uxuA; 1.
PE   3: Inferred from homology;
KW   Iron; Lyase; Manganese; Reference proteome.
FT   CHAIN           1..351
FT                   /note="Mannonate dehydratase"
FT                   /id="PRO_0000170668"
SQ   SEQUENCE   351 AA;  40460 MW;  B8FFBFEFDDF2F383 CRC64;
     MNLSFRWYGA DDAVKLQYIR QIPSIKSIVT AIYDVPVGEK WSIEAILKLK NEVESYGLNF
     DVIESVPVHE DIKLGLKTRD KYIENYKENI RNLSKAGVKV ICYNFMPIFD WTRTQVDKVL
     DDGSTTLVYY KNQLKKMDPL TGELSLPGWD SSYTKDQLSS LFEKYQNVDQ EVLWSNLEYF
     LKQIIPVAEE CDVKMAIHPD DPPYNIFGLP RIITNEQNLD RFLKIVDSKY NGLTFCTGSL
     GCASFNDVVK MVDKYSSQGR IHFMHVRNVK LLFDGSFEES AHYSPCGSLD IVEIMKVLHK
     NKFDGYIRPD HGRMIWGETG RPGYGLYDRA LGAMYITGIW ETLEKISKED K