ID   UXUA_GEOSE              Reviewed;         371 AA.
AC   Q9ZFL7; Q09LX9;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Mannonate dehydratase;
DE            EC=4.2.1.8;
DE   AltName: Full=D-mannonate hydro-lyase;
GN   Name=uxuA;
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=T-6;
RX   PubMed=10368143; DOI=10.1128/jb.181.12.3695-3704.1999;
RA   Shulami S., Gat O., Sonenshein A.L., Shoham Y.;
RT   "The glucuronic acid utilization gene cluster from Bacillus
RT   stearothermophilus T-6.";
RL   J. Bacteriol. 181:3695-3704(1999).
CC   -!- FUNCTION: Catalyzes the dehydration of D-mannonate. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O;
CC         Xref=Rhea:RHEA:20097, ChEBI:CHEBI:15377, ChEBI:CHEBI:17767,
CC         ChEBI:CHEBI:57990; EC=4.2.1.8;
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000250};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- PATHWAY: Carbohydrate metabolism; pentose and glucuronate
CC       interconversion.
CC   -!- SIMILARITY: Belongs to the mannonate dehydratase family. {ECO:0000305}.
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DR   EMBL; DQ868502; ABI49946.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9ZFL7; -.
DR   SMR; Q9ZFL7; -.
DR   UniPathway; UPA00246; -.
DR   GO; GO:0008927; F:mannonate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006064; P:glucuronate catabolic process; IEA:InterPro.
DR   HAMAP; MF_00106; UxuA; 1.
DR   InterPro; IPR004628; Man_deHydtase.
DR   InterPro; IPR036237; Xyl_isomerase-like_sf.
DR   PANTHER; PTHR30387; PTHR30387; 1.
DR   Pfam; PF03786; UxuA; 1.
DR   PIRSF; PIRSF016049; Man_dehyd; 1.
DR   SUPFAM; SSF51658; SSF51658; 1.
DR   TIGRFAMs; TIGR00695; uxuA; 1.
PE   3: Inferred from homology;
KW   Iron; Lyase; Manganese.
FT   CHAIN           1..371
FT                   /note="Mannonate dehydratase"
FT                   /id="PRO_0000170663"
SQ   SEQUENCE   371 AA;  42532 MW;  DF82D85FACDAE944 CRC64;
     MKMTFRWFGK EHDTVSLDHI RQIPGVEGIV GALYHIPVGE VWPLDDILEL KRQVNEKGFH
     LEVIESVNVH EDIKLGLPSR DRYIENYKQT IRNLAKAGVK VICYNFMPIF DWTRSDLAKR
     RPDGSTVLAY EKQKIEQIDP EEMIRRIESG ANGFLLPGWE PERLKTIKPL FSLYKGVTEE
     DLFDHLRYFL EQIVPVAEEC GVRMALHPDD PPWSVFGLPR IATNKENLDR IVHMVNSPAN
     GLTLCSGSLG ANPANDVPDI FRHFLRMGRV PFAHVRNVEI HANGDFEETS HRSCDGSLNI
     CEIMKALHEA NFQGYIRPDH GRMIWGEQAR PGYGLYDRAL GIMYLLGIWD SLENEKKKQE
     GEKTCSRSIQ V