ID   UXUA_LACLM              Reviewed;         358 AA.
AC   A2RJK2;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2007, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Mannonate dehydratase {ECO:0000255|HAMAP-Rule:MF_00106};
DE            EC=4.2.1.8 {ECO:0000255|HAMAP-Rule:MF_00106};
DE   AltName: Full=D-mannonate hydro-lyase {ECO:0000255|HAMAP-Rule:MF_00106};
GN   Name=uxuA {ECO:0000255|HAMAP-Rule:MF_00106}; OrderedLocusNames=llmg_0859;
OS   Lactococcus lactis subsp. cremoris (strain MG1363).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus; Lactococcus cremoris subsp. cremoris.
OX   NCBI_TaxID=416870;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MG1363;
RX   PubMed=17307855; DOI=10.1128/jb.01768-06;
RA   Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C.,
RA   Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P.,
RA   van Sinderen D., Kok J.;
RT   "The complete genome sequence of the lactic acid bacterial paradigm
RT   Lactococcus lactis subsp. cremoris MG1363.";
RL   J. Bacteriol. 189:3256-3270(2007).
CC   -!- FUNCTION: Catalyzes the dehydration of D-mannonate. {ECO:0000255|HAMAP-
CC       Rule:MF_00106}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O;
CC         Xref=Rhea:RHEA:20097, ChEBI:CHEBI:15377, ChEBI:CHEBI:17767,
CC         ChEBI:CHEBI:57990; EC=4.2.1.8; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00106};
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00106};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00106};
CC   -!- PATHWAY: Carbohydrate metabolism; pentose and glucuronate
CC       interconversion. {ECO:0000255|HAMAP-Rule:MF_00106}.
CC   -!- SIMILARITY: Belongs to the mannonate dehydratase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00106}.
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DR   EMBL; AM406671; CAL97454.1; -; Genomic_DNA.
DR   RefSeq; WP_011834823.1; NZ_WJVF01000014.1.
DR   AlphaFoldDB; A2RJK2; -.
DR   SMR; A2RJK2; -.
DR   STRING; 416870.llmg_0859; -.
DR   EnsemblBacteria; CAL97454; CAL97454; llmg_0859.
DR   KEGG; llm:llmg_0859; -.
DR   eggNOG; COG1312; Bacteria.
DR   HOGENOM; CLU_058621_1_0_9; -.
DR   OMA; GIVWALH; -.
DR   PhylomeDB; A2RJK2; -.
DR   BioCyc; LLAC416870:LLMG_RS04395-MON; -.
DR   UniPathway; UPA00246; -.
DR   Proteomes; UP000000364; Chromosome.
DR   GO; GO:0008927; F:mannonate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006064; P:glucuronate catabolic process; IEA:InterPro.
DR   HAMAP; MF_00106; UxuA; 1.
DR   InterPro; IPR004628; Man_deHydtase.
DR   InterPro; IPR036237; Xyl_isomerase-like_sf.
DR   PANTHER; PTHR30387; PTHR30387; 1.
DR   Pfam; PF03786; UxuA; 1.
DR   PIRSF; PIRSF016049; Man_dehyd; 1.
DR   SUPFAM; SSF51658; SSF51658; 1.
DR   TIGRFAMs; TIGR00695; uxuA; 1.
PE   3: Inferred from homology;
KW   Iron; Lyase; Manganese.
FT   CHAIN           1..358
FT                   /note="Mannonate dehydratase"
FT                   /id="PRO_1000034333"
SQ   SEQUENCE   358 AA;  40811 MW;  BB62AE6F9C95D8D7 CRC64;
     MEMTMRWFGS NADKVELSEI AQVPGVKGVV GMIMDIPAGE VWPKERIKAL KEEIEAAGLS
     LKVIESVNIH DDIKIGLPTR DKYIENYKET IRNLAEFGIE VICYNFMPVF DWLKSDLDYR
     LADNSQTMAF VAEDIPENPQ EIIDRVQAAD GGFSLPGWEP ERLSEVKDLF EAYKNVDEHK
     LRENFAYFLK AIIPTCEEVG IKMAVHPDDP PYPMFGLPRV VKNREDLDWI CNVVDSPSNA
     ITLCTGSIAE DPANNVYEIM AEFVKRDRIP FAHVRNIKFL PSGEKDFYEA PHMSEYGSLD
     MYKILKAMYD NGFDGYIRPD HGRMIWGETG RPGYGLYDRA LGASYLNGLW EALEKNKQ