ID   UXUA_RHIME              Reviewed;         402 AA.
AC   Q92WB5;
DT   11-JUL-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Mannonate dehydratase {ECO:0000255|HAMAP-Rule:MF_00106};
DE            EC=4.2.1.8 {ECO:0000255|HAMAP-Rule:MF_00106};
DE   AltName: Full=D-mannonate hydro-lyase {ECO:0000255|HAMAP-Rule:MF_00106};
GN   Name=uxuA {ECO:0000255|HAMAP-Rule:MF_00106}; OrderedLocusNames=RB0428;
GN   ORFNames=SMb20446;
OS   Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS   meliloti).
OG   Plasmid pSymB (megaplasmid 2).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=266834;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11481431; DOI=10.1073/pnas.161294698;
RA   Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J.,
RA   Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
RT   "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing
RT   endosymbiont Sinorhizobium meliloti.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1021;
RX   PubMed=11474104; DOI=10.1126/science.1060966;
RA   Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA   Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA   Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA   Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA   Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA   Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA   Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA   Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA   Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA   Wong K., Yeh K.-C., Batut J.;
RT   "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL   Science 293:668-672(2001).
CC   -!- FUNCTION: Catalyzes the dehydration of D-mannonate. {ECO:0000255|HAMAP-
CC       Rule:MF_00106}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O;
CC         Xref=Rhea:RHEA:20097, ChEBI:CHEBI:15377, ChEBI:CHEBI:17767,
CC         ChEBI:CHEBI:57990; EC=4.2.1.8; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00106};
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00106};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00106};
CC   -!- PATHWAY: Carbohydrate metabolism; pentose and glucuronate
CC       interconversion. {ECO:0000255|HAMAP-Rule:MF_00106}.
CC   -!- SIMILARITY: Belongs to the mannonate dehydratase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00106}.
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DR   EMBL; AL591985; CAC48828.1; -; Genomic_DNA.
DR   PIR; D95895; D95895.
DR   RefSeq; NP_436968.1; NC_003078.1.
DR   RefSeq; WP_010975307.1; NC_003078.1.
DR   AlphaFoldDB; Q92WB5; -.
DR   SMR; Q92WB5; -.
DR   STRING; 266834.SM_b20446; -.
DR   EnsemblBacteria; CAC48828; CAC48828; SM_b20446.
DR   GeneID; 61600435; -.
DR   KEGG; sme:SM_b20446; -.
DR   PATRIC; fig|266834.11.peg.5358; -.
DR   eggNOG; COG1312; Bacteria.
DR   HOGENOM; CLU_058621_2_0_5; -.
DR   OMA; GIVWALH; -.
DR   UniPathway; UPA00246; -.
DR   Proteomes; UP000001976; Plasmid pSymB.
DR   GO; GO:0008927; F:mannonate dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006064; P:glucuronate catabolic process; IEA:InterPro.
DR   HAMAP; MF_00106; UxuA; 1.
DR   InterPro; IPR004628; Man_deHydtase.
DR   InterPro; IPR036237; Xyl_isomerase-like_sf.
DR   PANTHER; PTHR30387; PTHR30387; 1.
DR   Pfam; PF03786; UxuA; 1.
DR   PIRSF; PIRSF016049; Man_dehyd; 1.
DR   SUPFAM; SSF51658; SSF51658; 1.
DR   TIGRFAMs; TIGR00695; uxuA; 1.
PE   3: Inferred from homology;
KW   Iron; Lyase; Manganese; Plasmid; Reference proteome.
FT   CHAIN           1..402
FT                   /note="Mannonate dehydratase"
FT                   /id="PRO_0000170683"
SQ   SEQUENCE   402 AA;  45242 MW;  8DD4ED1B685716DA CRC64;
     MRHTWRWFGP VDRVSVQDAA QAGAHGIVSA LHHIPTGDVW PVDEIGKRQE EVRAGGLEWE
     VVESVPVSEC IKTQTGPWRE HVANWQETLR RLSAAGIRTV CYNFMPVLDW TRTDLRWTAR
     HGAKAMRFDR IDFAAFDIHL LERPAAHEDY DTATREAAER RFREMTEERR LALSRNIGAG
     LPGSADGYSL PQLREHLRTY DGVSREKLQR HLVEFLAEVA PVAERTGINI CAHPDDPPWA
     LLGLPRILSN AEDYAFMLRE VDSPANGVTL CTGSLGALAA NDLPAMTRQF ASRIHFVHLR
     NVLREEVRTP CSFYEDEHLE GDTDMVAVIA ELLQEEQRRR AAGRVDHQIP MRPDHGQEIL
     DDLSRGAQPG YPAIGRLKGL AELRGIERAL SHATYGLSAA TR