ID   V143_NPVOP              Reviewed;        1223 AA.
AC   Q83950; O12557; O12846;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=ATP-dependent DNA helicase P143;
DE            EC=3.6.4.12;
GN   Name=P143; ORFNames=ORF96;
OS   Orgyia pseudotsugata multicapsid polyhedrosis virus (OpMNPV).
OC   Viruses; Naldaviricetes; Lefavirales; Baculoviridae; Alphabaculovirus.
OX   NCBI_TaxID=262177;
OH   NCBI_TaxID=33414; Orgyia pseudotsugata (Douglas-fir tussock moth).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8609478; DOI=10.1099/0022-1317-77-5-825;
RA   Ahrens C.H., Rohrmann G.F.;
RT   "The DNA polymerase and helicase genes of a baculovirus of Orgyia
RT   pseudosugata.";
RL   J. Gen. Virol. 77:825-837(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=9126251; DOI=10.1006/viro.1997.8448;
RA   Ahrens C.H., Russell R.R., Funk C.J., Evans J., Harwood S., Rohrmann G.F.;
RT   "The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear
RT   polyhedrosis virus genome.";
RL   Virology 229:381-399(1997).
CC   -!- FUNCTION: Essential for the initiation of viral DNA replication, it may
CC       contribute to other functions such as controlling the switch to the
CC       late phase and leading to the inhibition of host protein synthesis.
CC       Required for late and very late gene expression (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000305}.
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DR   EMBL; U39146; AAB60602.1; -; Genomic_DNA.
DR   EMBL; U75930; AAC59095.1; -; Genomic_DNA.
DR   RefSeq; NP_046252.1; NC_001875.2.
DR   GeneID; 912107; -.
DR   KEGG; vg:912107; -.
DR   Proteomes; UP000009248; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0019079; P:viral genome replication; ISS:UniProtKB.
DR   InterPro; IPR006824; DNA_helicase_Baculovir.
DR   Pfam; PF04735; Baculo_helicase; 1.
PE   3: Inferred from homology;
KW   ATP-binding; DNA replication; DNA-binding; Early protein; Helicase;
KW   Host nucleus; Hydrolase; Nucleotide-binding.
FT   CHAIN           1..1223
FT                   /note="ATP-dependent DNA helicase P143"
FT                   /id="PRO_0000132854"
FT   DNA_BIND        967..981
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255"
FT   MOTIF           692..701
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   BINDING         919..926
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1223 AA;  140497 MW;  88AA3A9A99936DDC CRC64;
     MNSILSRLFR DVTGDEEYAV NSLRDANRLI IIDTDTGTRR LLEHVSNFRR FLNTIRSDAA
     GACARHQRAA RHDDEEEAPP AARVSFAGHS LVLENNDFCV FVKPFLLKRH YDEIKNYLKL
     DRFFKSDNPE HTNMCVQAGD YCYWPNWPAS QAVSFTGWQL YLYVEFGIVV ESTVPIIHNR
     RLGPVDLFVF NPRTFLNIEM NLSTNEAPPV KLFVNGKFDF EKKVAESGKA VESLFEIKMA
     NGATATCKMI ANLVNSNKNL FEVIRDNINL EECVTTPKYR HIIDVNLTKL RQFAHDGAVL
     APPVERAFRA PTVTTILSAS SENAEVIQSE IDAALVKVRE GMVKVMAEFN RSDDPDLLQA
     YFKESGFKNF HFLLFNVWKQ ITKRDKKSFR ETDMKLFFEL VCETLFGNDS DALATALVKC
     EPFTTRGVAT FNSLCDHWHC FKGVNPYTLL GSYYGAHYFI YLKLTSSDAH ECDDPWAFNY
     KNAMECKVPP KALGEAFFIK VENVVTQVTL VFNGEHYQIV KKDDELYKLV ETNPYKLQNI
     KFNNWKYMYH TKYGVYNVVT DDFYSNCPFL LGTTMPGTFK RPDDPPYLPE AVFAHMLATS
     AEERDILRTY HIAKLCRDVK MVKVNLGTVN LLGDCAPCKL DARLRLNDLF RELWNLDDDS
     LVTLALYVNK LRVEDIVHNF KCGACRASVQ ERKCRCVQKI KINRQALKTC LIFDLFVGDP
     ELTQLMWMLI FATNKLYINT ALIVTTSKLV AQHAHFFTKE HIKIAAILHR DLHKIEFVDT
     LMADVCNHDA FLAYLQHAVA NEPAAAPAAD NAVAKFYAHY ANAANILHKY KNLWWDKIIL
     ARDSDTLSSW LTRFYLRVIL SKMDVQNYPV AYLTQVVEGY LYFKRYTNFN HASSYMLMHF
     AASLSVPTDY GRKAVYLPGV PLSGKSTFFE LLDFLVLMHK FDDDTHTGAS KETSDKEVSN
     LNSEVYTINE LKKCSESFFK KHADSSKSDS KSRKYQGLLK YEANYKMLIV NNNPLYVDDY
     DDGVQNRFLI VYTDHKFLPH VRFSGSVYHH ILTKQYPQEP MVVDALKDSV RVFLAHVVRY
     QREPQTGLVP YKTLLDSDPV HQHNLTRLSV NNSPMYAVIY ILNIKTAPRS ANTFVTEEKM
     QEMIGYATLH LKSFLHPSFT QYNAAKNINA GTARSFVFDE KILLQQIKDK FKNNYDERGC
     KFNNLTMALN KLDININVPQ FKC