V1AR_RAT
ID V1AR_RAT Reviewed; 424 AA.
AC P30560; Q5M8D1; Q62874; Q9QW18;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2006, sequence version 4.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Vasopressin V1a receptor;
DE Short=V1aR;
DE AltName: Full=AVPR V1a;
DE AltName: Full=Antidiuretic hormone receptor 1a;
DE AltName: Full=Vascular/hepatic-type arginine vasopressin receptor;
GN Name=Avpr1a;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Liver;
RX PubMed=1560825; DOI=10.1038/356523a0;
RA Morel A., O'Carroll A.-M., Brownstein M.J., Lolait S.J.;
RT "Molecular cloning and expression of a rat V1a arginine vasopressin
RT receptor.";
RL Nature 356:523-526(1992).
RN [2]
RP SEQUENCE REVISION TO 112-117.
RX PubMed=8257445; DOI=10.1042/bj2960519;
RA Wheatley M., Howl J., Morel A., Davis A.R.L.;
RT "Homology between neurohypophyseal hormone receptors.";
RL Biochem. J. 296:519-519(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Liver;
RX PubMed=8670090;
RA Innamorati G., Lolait S.J., Birnbaumer M.;
RT "Sequence identity between the rat and human vasopressin V1a receptors.";
RL Biochem. J. 314:710-711(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-395.
RX PubMed=8511367; DOI=10.1016/0167-0115(93)90182-8;
RA Morel A., Lolait S.J., Brownstein M.J.;
RT "Molecular cloning and expression of rat V1a and V2 arginine vasopressin
RT receptors.";
RL Regul. Pept. 45:53-59(1993).
RN [6]
RP MUTAGENESIS OF TYR-115.
RX PubMed=7774575; DOI=10.1002/j.1460-2075.1995.tb07211.x;
RA Chini B., Mouillac B., Ala Y., Balestre M.-N., Trumpp-Kallmeyer S.,
RA Hoflack J., Elands J., Hibert M., Manning M., Jard S., Barberis C.;
RT "Tyr115 is the key residue for determining agonist selectivity in the V1a
RT vasopressin receptor.";
RL EMBO J. 14:2176-2182(1995).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=9105680; DOI=10.1016/s0169-328x(96)00285-9;
RA Yamazaki R.S., Chen Q., Schreiber S.S., Brinton R.D.;
RT "Localization of V1a vasopressin receptor mRNA expression in cultured
RT neurons, astroglia, and oligodendroglia of rat cerebral cortex.";
RL Brain Res. Mol. Brain Res. 45:138-140(1997).
RN [8]
RP MUTAGENESIS OF ASN-14 AND ASN-27, AND SUBCELLULAR LOCATION.
RX PubMed=11520055; DOI=10.1006/bbrc.2001.5456;
RA Lee K.-H., Ahn J., Yu D.-H., Jeong H.-S., Lee S.-H., Kim K.-S.,
RA Chung I.-Y., Kim J.-H., Lee Y.-S.;
RT "Effect of N-glycosylation on ligand binding affinity of rat V1a
RT vasopressin receptor.";
RL Biochem. Biophys. Res. Commun. 286:707-713(2001).
RN [9]
RP PALMITOYLATION AT CYS-371 AND CYS-372, AND MUTAGENESIS OF CYS-371 AND
RP CYS-372.
RX PubMed=11466323; DOI=10.1074/jbc.m106142200;
RA Hawtin S.R., Tobin A.B., Patel S., Wheatley M.;
RT "Palmitoylation of the vasopressin V1a receptor reveals different
RT conformational requirements for signaling, agonist-induced receptor
RT phosphorylation, and sequestration.";
RL J. Biol. Chem. 276:38139-38146(2001).
RN [10]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=12399435; DOI=10.1210/en.2002-220603;
RA Orcel H., Tobin V.A., Alonso G., Rabie A.;
RT "Immunocytochemical localization of vasopressin v1a receptors in the rat
RT pituitary gonadotropes.";
RL Endocrinology 143:4385-4388(2002).
RN [11]
RP ROLE IN SOCIAL BEHAVIOR.
RX PubMed=12887422; DOI=10.1046/j.1460-9568.2003.02750.x;
RA Landgraf R., Frank E., Aldag J.M., Neumann I.D., Sharer C.A., Ren X.,
RA Terwilliger E.F., Niwa M., Wigger A., Young L.J.;
RT "Viral vector-mediated gene transfer of the vole V1a vasopressin receptor
RT in the rat septum: improved social discrimination and active social
RT behaviour.";
RL Eur. J. Neurosci. 18:403-411(2003).
CC -!- FUNCTION: Receptor for arginine vasopressin. The activity of this
CC receptor is mediated by G proteins which activate a phosphatidyl-
CC inositol-calcium second messenger system. Involved in social memory
CC formation. {ECO:0000269|PubMed:12887422}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note=Located
CC in cytoplasmic vesicles dispersed throughout the cell cytoplasm and to
CC the plasma membrane.
CC -!- TISSUE SPECIFICITY: Localized within gonadotropes of the anterior
CC pituitary of the brain. Broadly distributed throughout the cerebral
CC cortex. {ECO:0000269|PubMed:12399435, ECO:0000269|PubMed:9105680}.
CC -!- PTM: Palmitoylated on three cysteine residues, of which only two are
CC identified. {ECO:0000269|PubMed:11466323}.
CC -!- MISCELLANEOUS: Overexpression of AVPR1A in the brain increases the
CC duration of social memory.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Vasopressin/oxytocin receptor subfamily. {ECO:0000255|PROSITE-
CC ProRule:PRU00521}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA77748.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; Z11690; CAA77748.1; ALT_INIT; mRNA.
DR EMBL; U39450; AAC52507.1; -; Genomic_DNA.
DR EMBL; BC088095; AAH88095.1; -; Genomic_DNA.
DR PIR; S71837; S71837.
DR RefSeq; NP_444178.2; NM_053019.2.
DR AlphaFoldDB; P30560; -.
DR SMR; P30560; -.
DR IntAct; P30560; 2.
DR STRING; 10116.ENSRNOP00000005829; -.
DR BindingDB; P30560; -.
DR ChEMBL; CHEMBL2868; -.
DR DrugCentral; P30560; -.
DR GuidetoPHARMACOLOGY; 366; -.
DR GlyGen; P30560; 1 site.
DR PhosphoSitePlus; P30560; -.
DR SwissPalm; P30560; -.
DR PaxDb; P30560; -.
DR Ensembl; ENSRNOT00000005829; ENSRNOP00000005829; ENSRNOG00000004400.
DR GeneID; 25107; -.
DR KEGG; rno:25107; -.
DR CTD; 552; -.
DR RGD; 2185; Avpr1a.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01050000244882; -.
DR InParanoid; P30560; -.
DR OMA; RFRGPDW; -.
DR OrthoDB; 890925at2759; -.
DR PhylomeDB; P30560; -.
DR TreeFam; TF106499; -.
DR Reactome; R-RNO-388479; Vasopressin-like receptors.
DR Reactome; R-RNO-416476; G alpha (q) signalling events.
DR PRO; PR:P30560; -.
DR Proteomes; UP000002494; Chromosome 7.
DR Bgee; ENSRNOG00000004400; Expressed in liver and 17 other tissues.
DR ExpressionAtlas; P30560; baseline and differential.
DR Genevisible; P30560; RN.
DR GO; GO:0031410; C:cytoplasmic vesicle; IDA:RGD.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030139; C:endocytic vesicle; ISO:RGD.
DR GO; GO:0005768; C:endosome; ISO:RGD.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:RGD.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0042277; F:peptide binding; IBA:GO_Central.
DR GO; GO:0017046; F:peptide hormone binding; IMP:RGD.
DR GO; GO:0031894; F:V1A vasopressin receptor binding; IDA:UniProtKB.
DR GO; GO:0005000; F:vasopressin receptor activity; IDA:RGD.
DR GO; GO:0019722; P:calcium-mediated signaling; IMP:RGD.
DR GO; GO:0042631; P:cellular response to water deprivation; IEP:RGD.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007625; P:grooming behavior; IMP:RGD.
DR GO; GO:0002125; P:maternal aggressive behavior; IMP:RGD.
DR GO; GO:0042711; P:maternal behavior; IMP:RGD.
DR GO; GO:0010259; P:multicellular organism aging; ISO:RGD.
DR GO; GO:0014902; P:myotube differentiation; IEP:RGD.
DR GO; GO:0007621; P:negative regulation of female receptivity; IMP:RGD.
DR GO; GO:0051970; P:negative regulation of transmission of nerve impulse; IMP:RGD.
DR GO; GO:0043084; P:penile erection; IMP:RGD.
DR GO; GO:0045777; P:positive regulation of blood pressure; IMP:RGD.
DR GO; GO:0030307; P:positive regulation of cell growth; IMP:RGD.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:RGD.
DR GO; GO:0032849; P:positive regulation of cellular pH reduction; IMP:RGD.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IMP:RGD.
DR GO; GO:0014049; P:positive regulation of glutamate secretion; IMP:RGD.
DR GO; GO:0045819; P:positive regulation of glycogen catabolic process; NAS:UniProtKB.
DR GO; GO:0010460; P:positive regulation of heart rate; IMP:RGD.
DR GO; GO:0031394; P:positive regulation of prostaglandin biosynthetic process; IMP:RGD.
DR GO; GO:0035815; P:positive regulation of renal sodium excretion; IMP:RGD.
DR GO; GO:0003084; P:positive regulation of systemic arterial blood pressure; IMP:RGD.
DR GO; GO:0035810; P:positive regulation of urine volume; NAS:UniProtKB.
DR GO; GO:0045907; P:positive regulation of vasoconstriction; IMP:RGD.
DR GO; GO:0008217; P:regulation of blood pressure; ISO:RGD.
DR GO; GO:0051459; P:regulation of corticotropin secretion; NAS:UniProtKB.
DR GO; GO:0001992; P:regulation of systemic arterial blood pressure by vasopressin; ISO:RGD.
DR GO; GO:0051412; P:response to corticosterone; IEP:RGD.
DR GO; GO:0010035; P:response to inorganic substance; IMP:RGD.
DR GO; GO:0010033; P:response to organic substance; IMP:RGD.
DR GO; GO:0035176; P:social behavior; IDA:UniProtKB.
DR GO; GO:0042713; P:sperm ejaculation; IMP:RGD.
DR GO; GO:0021537; P:telencephalon development; IEP:RGD.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR015076; V1R_C.
DR InterPro; IPR001817; Vasoprsn_rcpt.
DR InterPro; IPR001224; Vprs_V1A_rcpt.
DR Pfam; PF00001; 7tm_1; 1.
DR Pfam; PF08983; DUF1856; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00896; VASOPRESSINR.
DR PRINTS; PR00752; VASOPRSNV1AR.
DR SMART; SM01164; DUF1856; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cytoplasmic vesicle; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Lipoprotein; Membrane; Palmitate;
KW Phosphoprotein; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..424
FT /note="Vasopressin V1a receptor"
FT /id="PRO_0000070201"
FT TOPO_DOM 1..52
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..76
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..88
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..110
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 111..125
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..147
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..168
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..190
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 191..220
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..299
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..319
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 320..337
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..357
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 358..424
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 383..416
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 389..416
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 410
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q62463"
FT LIPID 371
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000269|PubMed:11466323"
FT LIPID 372
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000269|PubMed:11466323"
FT CARBOHYD 27
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 124..205
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT MUTAGEN 14
FT /note="N->Q: Reduced ligand binding affinity."
FT /evidence="ECO:0000269|PubMed:11520055"
FT MUTAGEN 27
FT /note="N->Q: Reduced ligand binding affinity."
FT /evidence="ECO:0000269|PubMed:11520055"
FT MUTAGEN 115
FT /note="Y->D: No significant change in affinity for arginine
FT vasopressin or oxytocin. Increased affinity for 1-deamino,
FT D-Arg8-vasopressin."
FT /evidence="ECO:0000269|PubMed:7774575"
FT MUTAGEN 115
FT /note="Y->F: 17-fold increase in affinity for oxytocin. No
FT change in affinity for arginine vasopressin or 1-deamino,
FT D-Arg8-vasopressin."
FT /evidence="ECO:0000269|PubMed:7774575"
FT MUTAGEN 115
FT /note="Y->L: 50-fold decrease in affinity for arginine
FT vasopressin. Little affect on affinity for oxytocin or 1-
FT deamino, D-Arg8-vasopressin."
FT /evidence="ECO:0000269|PubMed:7774575"
FT MUTAGEN 371
FT /note="C->G: Reduced palmitoylation, coupling to G protein
FT unaffected. Abolished isoprenylation, coupling to G protein
FT unaffected; when associated with G-372."
FT /evidence="ECO:0000269|PubMed:11466323"
FT MUTAGEN 372
FT /note="C->G: Reduced palmitoylation, coupling to G protein
FT unaffected. Abolished isoprenylation, coupling to G protein
FT unaffected; when associated with G-371."
FT /evidence="ECO:0000269|PubMed:11466323"
FT CONFLICT 115..116
FT /note="YR -> SS (in Ref. 3 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 390..424
FT /note="RQTSYSNNRSPTNSTGMWKDSPKSSKSIRFIPVST -> KTDFLF (in
FT Ref. 1; CAA77748)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 424 AA; 47655 MW; 4F452C8DF7B4C900 CRC64;
MSFPRGSQDR SVGNSSPWWP LTTEGSNGSQ EAARLGEGDS PLGDVRNEEL AKLEIAVLAV
IFVVAVLGNS SVLLALHRTP RKTSRMHLFI RHLSLADLAV AFFQVLPQLC WDITYRFRGP
DWLCRVVKHL QVFAMFASAY MLVVMTADRY IAVCHPLKTL QQPARRSRLM IATSWVLSFI
LSTPQYFIFS VIEIEVNNGT KTQDCWATFI QPWGTRAYVT WMTSGVFVAP VVVLGTCYGF
ICYHIWRNIR GKTASSRHSK GDKGSGEAVG PFHKGLLVTP CVSSVKSISR AKIRTVKMTF
VIVSAYILCW APFFIVQMWS VWDENFIWTD SENPSITITA LLASLNSCCN PWIYMFFSGH
LLQDCVQSFP CCHSMAQKFA KDDSDSMSRR QTSYSNNRSP TNSTGMWKDS PKSSKSIRFI
PVST
