VA0E1_ARATH
ID VA0E1_ARATH Reviewed; 70 AA.
AC Q9FLN5;
DT 01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=V-type proton ATPase subunit e1;
DE Short=V-ATPase subunit e1;
DE AltName: Full=Vacuolar H(+)-ATPase subunit e isoform 1;
DE AltName: Full=Vacuolar proton pump subunit e1;
GN Name=VHA-e1; Synonyms=VMA9; OrderedLocusNames=At5g55290; ORFNames=MCO15_24;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11950611; DOI=10.1016/s1360-1385(02)02240-9;
RA Sze H., Schumacher K., Mueller M.L., Padmanaban S., Taiz L.;
RT "A simple nomenclature for a complex proton pump: VHA genes encode the
RT vacuolar H(+)-ATPase.";
RL Trends Plant Sci. 7:157-161(2002).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=18507826; DOI=10.1186/1471-2121-9-28;
RA Seidel T., Schnitzer D., Golldack D., Sauer M., Dietz K.J.;
RT "Organelle-specific isoenzymes of plant V-ATPase as revealed by in vivo-
RT FRET analysis.";
RL BMC Cell Biol. 9:28-28(2008).
CC -!- FUNCTION: Subunit of the integral membrane V0 complex of vacuolar
CC ATPase. V-ATPase is responsible for acidifying a variety of
CC intracellular compartments in eukaryotic cells (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: V-ATPase is a heteromultimeric enzyme composed of a peripheral
CC catalytic V1 complex (components A to H) attached to an integral
CC membrane V0 proton pore complex (components: a, c, c'', d and e).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000269|PubMed:18507826}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:18507826}.
CC -!- SIMILARITY: Belongs to the V-ATPase e1/e2 subunit family.
CC {ECO:0000305}.
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DR EMBL; AB010071; BAB08598.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96610.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96611.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM71144.1; -; Genomic_DNA.
DR EMBL; AF372884; AAK49600.1; -; mRNA.
DR EMBL; AY057717; AAL15347.1; -; mRNA.
DR EMBL; AK318957; BAH57072.1; -; mRNA.
DR RefSeq; NP_001154781.1; NM_001161309.2.
DR RefSeq; NP_001332693.1; NM_001345122.1.
DR RefSeq; NP_568823.1; NM_124911.5.
DR AlphaFoldDB; Q9FLN5; -.
DR SMR; Q9FLN5; -.
DR BioGRID; 20866; 28.
DR IntAct; Q9FLN5; 28.
DR STRING; 3702.AT5G55290.1; -.
DR PaxDb; Q9FLN5; -.
DR EnsemblPlants; AT5G55290.1; AT5G55290.1; AT5G55290.
DR EnsemblPlants; AT5G55290.2; AT5G55290.2; AT5G55290.
DR EnsemblPlants; AT5G55290.3; AT5G55290.3; AT5G55290.
DR GeneID; 835622; -.
DR Gramene; AT5G55290.1; AT5G55290.1; AT5G55290.
DR Gramene; AT5G55290.2; AT5G55290.2; AT5G55290.
DR Gramene; AT5G55290.3; AT5G55290.3; AT5G55290.
DR KEGG; ath:AT5G55290; -.
DR Araport; AT5G55290; -.
DR TAIR; locus:2161625; AT5G55290.
DR eggNOG; KOG3500; Eukaryota.
DR HOGENOM; CLU_170555_2_0_1; -.
DR InParanoid; Q9FLN5; -.
DR OMA; SFCTRIC; -.
DR OrthoDB; 1613627at2759; -.
DR PhylomeDB; Q9FLN5; -.
DR PRO; PR:Q9FLN5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FLN5; baseline and differential.
DR Genevisible; Q9FLN5; AT.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0033179; C:proton-transporting V-type ATPase, V0 domain; IEA:InterPro.
DR GO; GO:0005773; C:vacuole; HDA:TAIR.
DR GO; GO:0046961; F:proton-transporting ATPase activity, rotational mechanism; IEA:InterPro.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR InterPro; IPR008389; ATPase_V0-cplx_e1/e2_su.
DR PANTHER; PTHR12263; PTHR12263; 1.
DR Pfam; PF05493; ATP_synt_H; 1.
PE 3: Inferred from homology;
KW Golgi apparatus; Hydrogen ion transport; Ion transport; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..70
FT /note="V-type proton ATPase subunit e1"
FT /id="PRO_0000430417"
FT TRANSMEM 1..21
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
SQ SEQUENCE 70 AA; 7726 MW; 4421F0524C9ED6BF CRC64;
MGFLITTLIF VVVGIIASLC VRICCNRGPS TNLLHLTLVI TATVCCWMMW AIVYIAQMNP
LIVPILSETE
