ID   VA2_SOLIN               Reviewed;         138 AA.
AC   P35775;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Venom allergen 2;
DE   AltName: Full=Allergen Sol i II;
DE   AltName: Full=Venom allergen II;
DE   AltName: Allergen=Sol i 2;
DE   Flags: Precursor;
OS   Solenopsis invicta (Red imported fire ant) (Solenopsis wagneri).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC   Formicidae; Myrmicinae; Solenopsis.
OX   NCBI_TaxID=13686;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=8454046; DOI=10.1016/0014-5793(93)80053-w;
RA   Schmidt M., Walker R.B., Hoffman D.R., McConnell T.J.;
RT   "Nucleotide sequence of cDNA encoding the fire ant venom protein Sol i
RT   II.";
RL   FEBS Lett. 319:138-140(1993).
RN   [2]
RP   PROTEIN SEQUENCE OF 20-138.
RC   TISSUE=Venom;
RX   PubMed=8423273; DOI=10.1016/0091-6749(93)90298-t;
RA   Hoffman D.R.;
RT   "Allergens in Hymenoptera venom XXIV: the amino acid sequences of imported
RT   fire ant venom allergens Sol i II, Sol i III, and Sol i IV.";
RL   J. Allergy Clin. Immunol. 91:71-78(1993).
RN   [3]
RP   PROTEIN SEQUENCE OF 20-87.
RC   TISSUE=Venom;
RX   PubMed=2355158; DOI=10.1016/0091-6749(90)90042-3;
RA   Hoffman D.R., Smith A.M., Schmidt M., Moffitt J.E., Guralnick M.;
RT   "Allergens in Hymenoptera venom. XXII. Comparison of venoms from two
RT   species of imported fire ants, Solenopsis invicta and richteri.";
RL   J. Allergy Clin. Immunol. 85:988-996(1990).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 20-138, SUBUNIT, AND DISULFIDE
RP   BONDS.
RX   PubMed=22100449; DOI=10.1016/j.jmb.2011.10.009;
RA   Borer A.S., Wassmann P., Schmidt M., Hoffman D.R., Zhou J.J., Wright C.,
RA   Schirmer T., Markovic-Housley Z.;
RT   "Crystal structure of Sol I 2: a major allergen from fire ant venom.";
RL   J. Mol. Biol. 415:635-648(2012).
CC   -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000269|PubMed:22100449}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- ALLERGEN: Causes an allergic reaction in human. The most common cause
CC       of insect venom allergy in the southeastern United States is the
CC       imported fire ant.
CC   -!- SIMILARITY: Belongs to the ant venom allergen 2/4 family.
CC       {ECO:0000305}.
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DR   EMBL; L09560; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; S32389; A37330.
DR   PDB; 2YGU; X-ray; 2.60 A; A/B/C/D/E/F/G/H=20-138.
DR   PDBsum; 2YGU; -.
DR   AlphaFoldDB; P35775; -.
DR   SMR; P35775; -.
DR   Allergome; 3482; Sol i 2.0101.
DR   Allergome; 631; Sol i 2.
DR   EnsemblMetazoa; XM_039454195.1; XP_039310129.1; LOC105205300.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   CDD; cd12800; Sol_i_2; 1.
DR   Gene3D; 1.10.238.190; -; 1.
DR   InterPro; IPR020181; Ant_venom_allergen_Sol_i_2.
DR   InterPro; IPR038211; Ant_venon_allerg_soli_2/4_sf.
PE   1: Evidence at protein level;
KW   3D-structure; Allergen; Direct protein sequencing; Disulfide bond;
KW   Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000269|PubMed:2355158,
FT                   ECO:0000269|PubMed:8423273"
FT   CHAIN           20..138
FT                   /note="Venom allergen 2"
FT                   /id="PRO_0000022646"
FT   DISULFID        34..57
FT                   /evidence="ECO:0000269|PubMed:22100449"
FT   DISULFID        41
FT                   /note="Interchain"
FT                   /evidence="ECO:0000269|PubMed:22100449"
FT   DISULFID        81..94
FT                   /evidence="ECO:0000269|PubMed:22100449"
FT   DISULFID        101..122
FT                   /evidence="ECO:0000269|PubMed:22100449"
FT   HELIX           21..37
FT                   /evidence="ECO:0007829|PDB:2YGU"
FT   HELIX           48..50
FT                   /evidence="ECO:0007829|PDB:2YGU"
FT   HELIX           52..61
FT                   /evidence="ECO:0007829|PDB:2YGU"
FT   TURN            62..65
FT                   /evidence="ECO:0007829|PDB:2YGU"
FT   HELIX           70..84
FT                   /evidence="ECO:0007829|PDB:2YGU"
FT   HELIX           88..105
FT                   /evidence="ECO:0007829|PDB:2YGU"
FT   STRAND          108..110
FT                   /evidence="ECO:0007829|PDB:2YGU"
FT   HELIX           112..125
FT                   /evidence="ECO:0007829|PDB:2YGU"
FT   HELIX           128..131
FT                   /evidence="ECO:0007829|PDB:2YGU"
SQ   SEQUENCE   138 AA;  15289 MW;  DA30E1E41041EE75 CRC64;
     MKSFVLATCL LGFAQIIYAD NKELKIIRKD VAECLRTLPK CGNQPDDPLA RVDVWHCAMA
     KRGVYDNPDP AVIKERSMKM CTKIITDPAN VENCKKVASR CVDRETQGPK SNRQKAVNII
     GCALRAGVAE TTVLARKK