VA3_SOLIN
ID VA3_SOLIN Reviewed; 234 AA.
AC P35778; O16135; Q9TWZ2;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Venom allergen 3;
DE AltName: Full=Allergen Sol i III {ECO:0000303|PubMed:8423273};
DE AltName: Full=Antigen 5 {ECO:0000305};
DE Short=Ag5 {ECO:0000305};
DE AltName: Full=Cysteine-rich venom protein {ECO:0000305};
DE Short=CRVP {ECO:0000305};
DE AltName: Full=Venom allergen III;
DE AltName: Allergen=Sol i 3 {ECO:0000303|PubMed:18761353};
DE Flags: Precursor;
OS Solenopsis invicta (Red imported fire ant) (Solenopsis wagneri).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC Formicidae; Myrmicinae; Solenopsis.
OX NCBI_TaxID=13686;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RA Hoffman D.R., Farrar D., Schmidt M., McConnell T.J.;
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 23-234.
RC TISSUE=Venom gland;
RX PubMed=8588684; DOI=10.1111/j.1398-9995.1995.tb01196.x;
RA Hoffman D.R.;
RT "Fire ant venom allergy.";
RL Allergy 50:535-544(1995).
RN [3]
RP PROTEIN SEQUENCE OF 23-234, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=8423273; DOI=10.1016/0091-6749(93)90298-t;
RA Hoffman D.R.;
RT "Allergens in Hymenoptera venom XXIV: the amino acid sequences of imported
RT fire ant venom allergens Sol i II, Sol i III, and Sol i IV.";
RL J. Allergy Clin. Immunol. 91:71-78(1993).
RN [4]
RP PROTEIN SEQUENCE OF 23-45, AND SUBCELLULAR LOCATION.
RC TISSUE=Venom;
RX PubMed=2355158; DOI=10.1016/0091-6749(90)90042-3;
RA Hoffman D.R., Smith A.M., Schmidt M., Moffitt J.E., Guralnick M.;
RT "Allergens in Hymenoptera venom. XXII. Comparison of venoms from two
RT species of imported fire ants, Solenopsis invicta and richteri.";
RL J. Allergy Clin. Immunol. 85:988-996(1990).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) OF 23-234, AND DISULFIDE BONDS.
RX PubMed=18761353; DOI=10.1016/j.jmb.2008.08.023;
RA Padavattan S., Schmidt M., Hoffman D.R., Markovic-Housley Z.;
RT "Crystal structure of the major allergen from fire ant venom, Sol i 3.";
RL J. Mol. Biol. 383:178-185(2008).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:2355158,
CC ECO:0000269|PubMed:8423273}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:2355158, ECO:0000305|PubMed:8423273}.
CC -!- ALLERGEN: Causes an allergic reaction in human. The most common cause
CC of insect venom allergy in the southeastern United States is the
CC imported fire ant. {ECO:0000269|PubMed:8423273}.
CC -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR EMBL; AF012919; AAB65434.1; -; mRNA.
DR PIR; C44582; B37330.
DR RefSeq; NP_001291520.1; NM_001304591.1.
DR PDB; 2VZN; X-ray; 3.05 A; A/B=23-234.
DR PDBsum; 2VZN; -.
DR AlphaFoldDB; P35778; -.
DR SMR; P35778; -.
DR Allergome; 3483; Sol i 3.0101.
DR Allergome; 632; Sol i 3.
DR PRIDE; P35778; -.
DR EnsemblMetazoa; NM_001304591.1; NP_001291520.1; LOC105199703.
DR GeneID; 105199703; -.
DR KEGG; soc:105199703; -.
DR HOGENOM; CLU_035730_7_2_1; -.
DR OMA; YSCEIED; -.
DR OrthoDB; 1528782at2759; -.
DR EvolutionaryTrace; P35778; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR001283; CRISP-related.
DR InterPro; IPR034763; P14a_insect.
DR InterPro; IPR002413; V5_allergen-like.
DR PANTHER; PTHR10334; PTHR10334; 1.
DR Pfam; PF00188; CAP; 1.
DR PIRSF; PIRSF038921; P14a; 1.
DR PRINTS; PR00838; V5ALLERGEN.
DR PRINTS; PR00837; V5TPXLIKE.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
DR PROSITE; PS01009; CRISP_1; 1.
DR PROSITE; PS01010; CRISP_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Direct protein sequencing; Disulfide bond;
KW Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000269|PubMed:2355158,
FT ECO:0000269|PubMed:8423273"
FT CHAIN 23..234
FT /note="Venom allergen 3"
FT /evidence="ECO:0000269|PubMed:8423273"
FT /id="PRO_0000006293"
FT DOMAIN 69..218
FT /note="SCP"
FT REGION 80..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 26..41
FT /evidence="ECO:0000269|PubMed:18761353"
FT DISULFID 31..125
FT /evidence="ECO:0000269|PubMed:18761353"
FT DISULFID 52..118
FT /evidence="ECO:0000269|PubMed:18761353"
FT DISULFID 198..216
FT /evidence="ECO:0000269|PubMed:18761353"
FT CONFLICT 183
FT /note="G -> E (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 194
FT /note="T -> S (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 199
FT /note="G -> A (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 29..33
FT /evidence="ECO:0007829|PDB:2VZN"
FT HELIX 39..42
FT /evidence="ECO:0007829|PDB:2VZN"
FT HELIX 62..80
FT /evidence="ECO:0007829|PDB:2VZN"
FT HELIX 105..115
FT /evidence="ECO:0007829|PDB:2VZN"
FT STRAND 129..131
FT /evidence="ECO:0007829|PDB:2VZN"
FT STRAND 134..142
FT /evidence="ECO:0007829|PDB:2VZN"
FT HELIX 150..158
FT /evidence="ECO:0007829|PDB:2VZN"
FT HELIX 159..163
FT /evidence="ECO:0007829|PDB:2VZN"
FT HELIX 166..168
FT /evidence="ECO:0007829|PDB:2VZN"
FT STRAND 169..171
FT /evidence="ECO:0007829|PDB:2VZN"
FT HELIX 176..188
FT /evidence="ECO:0007829|PDB:2VZN"
FT STRAND 195..204
FT /evidence="ECO:0007829|PDB:2VZN"
FT STRAND 210..220
FT /evidence="ECO:0007829|PDB:2VZN"
SQ SEQUENCE 234 AA; 26351 MW; 539F510B59941D83 CRC64;
MELIVSILWL AITAENLANT LATNYCNLQS CKRNNAIHTM CQYTSPTPGP MCLEYSNVGF
TDAEKDAIVN KHNELRQRVA SGKEMRGTNG PQPPAVKMPN LTWDPELATI AQRWANQCTF
EHDACRNVER FAVGQNIAAT SSSGKNKSTP NEMILLWYNE VKDFDNRWIS SFPSDDNILM
KVGHYTQIVW AKTTKIGCGR IMFKEPDNWT KHYLVCNYGP AGNVLGAPIY EIKK
