ID   VA52_DOLMA              Reviewed;         227 AA.
AC   P10736;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Venom allergen 5.01;
DE   AltName: Full=Allergen Dol m V-A;
DE   AltName: Full=Antigen 5 form 2;
DE            Short=Ag5-2;
DE   AltName: Full=Cysteine-rich venom protein;
DE            Short=CRVP;
DE   AltName: Allergen=Dol m 5.01;
DE   Flags: Precursor;
OS   Dolichovespula maculata (Bald-faced hornet) (Vespula maculata).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Vespoidea;
OC   Vespidae; Vespinae; Dolichovespula.
OX   NCBI_TaxID=7441;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=3422469; DOI=10.1073/pnas.85.3.895;
RA   Fang K.S.Y., Vitale M., Fehlner P., King T.P.;
RT   "cDNA cloning and primary structure of a white-face hornet venom allergen,
RT   antigen 5.";
RL   Proc. Natl. Acad. Sci. U.S.A. 85:895-899(1988).
RN   [2]
RP   PARTIAL PROTEIN SEQUENCE, AND DISULFIDE BONDS.
RC   TISSUE=Venom;
RX   PubMed=2402482;
RA   King T.P., Moran D., Wang D.F., Kochoumian L., Chait B.T.;
RT   "Structural studies of a hornet venom allergen antigen 5, Dol m V and its
RT   sequence similarity with other proteins.";
RL   Protein Seq. Data Anal. 3:263-266(1990).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- ALLERGEN: Causes an allergic reaction in human.
CC   -!- SIMILARITY: Belongs to the CRISP family. Venom allergen 5-like
CC       subfamily. {ECO:0000305}.
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DR   EMBL; J03601; AAA28301.1; -; mRNA.
DR   PIR; A31085; A31085.
DR   AlphaFoldDB; P10736; -.
DR   SMR; P10736; -.
DR   Allergome; 1656; Dol m 5.0101.
DR   Allergome; 330; Dol m 5.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.40.33.10; -; 1.
DR   InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR   InterPro; IPR014044; CAP_domain.
DR   InterPro; IPR035940; CAP_sf.
DR   InterPro; IPR001283; CRISP-related.
DR   InterPro; IPR002413; V5_allergen-like.
DR   PANTHER; PTHR10334; PTHR10334; 1.
DR   Pfam; PF00188; CAP; 1.
DR   PRINTS; PR00838; V5ALLERGEN.
DR   PRINTS; PR00837; V5TPXLIKE.
DR   SMART; SM00198; SCP; 1.
DR   SUPFAM; SSF55797; SSF55797; 1.
DR   PROSITE; PS01009; CRISP_1; 1.
DR   PROSITE; PS01010; CRISP_2; 1.
PE   1: Evidence at protein level;
KW   Allergen; Direct protein sequencing; Disulfide bond; Secreted; Signal.
FT   SIGNAL          1..23
FT   CHAIN           24..227
FT                   /note="Venom allergen 5.01"
FT                   /id="PRO_0000006294"
FT   DOMAIN          68..212
FT                   /note="SCP"
FT   DISULFID        27..39
FT                   /evidence="ECO:0000269|PubMed:2402482"
FT   DISULFID        31..124
FT                   /evidence="ECO:0000269|PubMed:2402482"
FT   DISULFID        49..117
FT                   /evidence="ECO:0000269|PubMed:2402482"
FT   DISULFID        193..210
FT                   /evidence="ECO:0000269|PubMed:2402482"
FT   VARIANT         54
FT                   /note="V -> A"
SQ   SEQUENCE   227 AA;  26000 MW;  4F0ED10D58C44502 CRC64;
     MEIGGLVYLI LIITIINLSF GETNNYCKIK CRKGIHTLCK FGTSMKPNCG RNVVKAYGLT
     NDEKNEILKR HNDFRQNVAK GLETRGKPGP QPPAKNMNVL VWNDELAKIA QTWANQCDFN
     HDDCRNTAKY QVGQNIAISS TTATQFDRPS KLIKQWEDEV TEFNYKVGLQ NSNFRKVGHY
     TQMVWGKTKE IGCGSIKYIE DNWYTHYLVC NYGPGGNDFN QPIYERK