VA5_POLPI
ID VA5_POLPI Reviewed; 207 AA.
AC P86686;
DT 10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT 10-AUG-2010, sequence version 1.
DT 25-MAY-2022, entry version 27.
DE RecName: Full=Venom allergen 5 {ECO:0000250|UniProtKB:P10736};
DE AltName: Full=Antigen 5 {ECO:0000303|Ref.1};
DE Short=Ag5 {ECO:0000250|UniProtKB:P10736};
DE AltName: Full=Cysteine-rich venom protein;
DE Short=CRVP;
DE AltName: Allergen=Poly p 5 {ECO:0000305};
OS Polybia paulista (Neotropical social wasp) (Swarm-founding polistine wasp).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Vespoidea;
OC Vespidae; Polistinae; Epiponini; Polybia.
OX NCBI_TaxID=291283;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS
RP SPECTROMETRY, AND ALLERGEN.
RC TISSUE=Venom {ECO:0000269|Ref.1};
RA Santos L.D.;
RT "Proteomic analysis of immunodominant allergens from the venom of the
RT social wasp Polybia paulista.";
RL Thesis (2007), Sao Paulo State University, Brazil.
RN [2]
RP PROTEIN SEQUENCE OF 1-14 AND 200-207, SUBCELLULAR LOCATION, MASS
RP SPECTROMETRY, IDENTIFICATION BY MASS SPECTROMETRY, GLYCATION AT LYS-141,
RP AND PHOSPHORYLATION AT TYR-111.
RC TISSUE=Venom {ECO:0000303|PubMed:24308509};
RX PubMed=24308509; DOI=10.1021/pr4008927;
RA Dos Santos-Pinto J.R., Dos Santos L.D., Andrade Arcuri H., Castro F.M.,
RA Kalil J.E., Palma M.S.;
RT "Using proteomic strategies for sequencing and post-translational
RT modifications assignment of antigen-5, a major allergen from the venom of
RT the social wasp Polybia paulista.";
RL J. Proteome Res. 13:855-865(2014).
RN [3]
RP SUBCELLULAR LOCATION, GLYCOSYLATION, MASS SPECTROMETRY, AND IDENTIFICATION
RP BY MASS SPECTROMETRY.
RC TISSUE=Venom {ECO:0000303|PubMed:20540563};
RX PubMed=20540563; DOI=10.1021/pr1000829;
RA dos Santos L.D., Santos K.S., Pinto J.R., Dias N.B., de Souza B.M.,
RA dos Santos M.F., Perales J., Domont G.B., Castro F.M., Kalil J.E.,
RA Palma M.S.;
RT "Profiling the proteome of the venom from the social wasp Polybia paulista:
RT a clue to understand the envenoming mechanism.";
RL J. Proteome Res. 9:3867-3877(2010).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20540563,
CC ECO:0000269|PubMed:24308509, ECO:0000269|Ref.1}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305|Ref.1}.
CC -!- PTM: Glycosylated. {ECO:0000269|PubMed:20540563}.
CC -!- MASS SPECTROMETRY: Mass=23009.2; Mass_error=0.5; Method=MALDI;
CC Evidence={ECO:0000269|Ref.1};
CC -!- MASS SPECTROMETRY: Mass=26479; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:24308509};
CC -!- MASS SPECTROMETRY: Mass=26479; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:20540563};
CC -!- ALLERGEN: Causes an allergic reaction in human (Ref.1,
CC PubMed:24308509). Binds to IgE (PubMed:24308509).
CC {ECO:0000269|PubMed:24308509, ECO:0000269|Ref.1}.
CC -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is: 9.7,
CC its MW is: 26.5 kDa. {ECO:0000269|Ref.1}.
CC -!- SIMILARITY: Belongs to the CRISP family. Venom allergen 5-like
CC subfamily. {ECO:0000305}.
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DR AlphaFoldDB; P86686; -.
DR SMR; P86686; -.
DR Allergome; 12001; Poly p 5.0102.
DR Allergome; 8738; Poly p 5.
DR iPTMnet; P86686; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR001283; CRISP-related.
DR InterPro; IPR002413; V5_allergen-like.
DR PANTHER; PTHR10334; PTHR10334; 1.
DR Pfam; PF00188; CAP; 1.
DR PRINTS; PR00838; V5ALLERGEN.
DR PRINTS; PR00837; V5TPXLIKE.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
DR PROSITE; PS01009; CRISP_1; 1.
DR PROSITE; PS01010; CRISP_2; 1.
PE 1: Evidence at protein level;
KW Allergen; Direct protein sequencing; Disulfide bond; Glycation;
KW Glycoprotein; Phosphoprotein; Secreted.
FT CHAIN 1..207
FT /note="Venom allergen 5"
FT /evidence="ECO:0000269|Ref.1"
FT /id="PRO_0000396652"
FT DOMAIN 49..192
FT /note="SCP"
FT /evidence="ECO:0000255"
FT MOD_RES 111
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000269|PubMed:24308509"
FT CARBOHYD 141
FT /note="N-linked (Glc) (glycation) lysine"
FT /evidence="ECO:0000269|PubMed:24308509"
FT DISULFID 4..16
FT /evidence="ECO:0000250|UniProtKB:P10736"
FT DISULFID 8..105
FT /evidence="ECO:0000250|UniProtKB:P10736"
FT DISULFID 29..97
FT /evidence="ECO:0000250|UniProtKB:P10736"
FT DISULFID 173..190
FT /evidence="ECO:0000250|UniProtKB:P10736"
SQ SEQUENCE 207 AA; 23009 MW; 60F8E9A321AC1879 CRC64;
NKYCNIKCSK VAHTVCQTGE STKPSSKNCA KVSITSVGVT EEEKKLIVDE HNRFRQKVAQ
GLETRGNPGP QPAASDMNNL VWNDELAYIA QVWASQCQFF VHDKCRNTAQ YQVGQNIAYS
ASTAAYPGVV KLIVLWENEV KDFNYNTGIT KENFAKVGHY TQVVWAKTKE VGCGSIKYIE
KGMKSHYLVC NYGPAGNVLG AQIYEIK
