ID   VA5_VESVE               Reviewed;         202 AA.
AC   P0DMB9;
DT   22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT   17-JUN-2020, sequence version 2.
DT   25-MAY-2022, entry version 15.
DE   RecName: Full=Venom allergen 5 {ECO:0000305};
DE   AltName: Full=Antigen 5 {ECO:0000303|PubMed:10484737};
DE   AltName: Allergen=Vesp v 5 {ECO:0000303|PubMed:31923175};
OS   Vespa velutina (Asian yellow-legged hornet).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Vespoidea;
OC   Vespidae; Vespinae; Vespa.
OX   NCBI_TaxID=202808 {ECO:0000303|PubMed:25896434};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Venom gland {ECO:0000303|PubMed:25896434};
RX   PubMed=25896434; DOI=10.1038/srep09454;
RA   Liu Z., Chen S., Zhou Y., Xie C., Zhu B., Zhu H., Liu S., Wang W., Chen H.,
RA   Ji Y.;
RT   "Deciphering the venomic transcriptome of killer-wasp Vespa velutina.";
RL   Sci. Rep. 5:9454-9454(2015).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-24, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=10484737; DOI=10.1016/s0041-0101(98)00229-3;
RA   Ho C.L., Lin Y.L., Li S.F.;
RT   "Three toxins with phospholipase activity isolated from the yellow-legged
RT   hornet (Vespa verutina) venom.";
RL   Toxicon 37:1015-1024(1999).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-5, SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom {ECO:0000303|PubMed:25896434};
RX   PubMed=31923175; DOI=10.1371/journal.pone.0225672;
RA   Monsalve R.I., Gutierrez R., Hoof I., Lombardero M.;
RT   "Purification and molecular characterization of phospholipase, antigen 5
RT   and hyaluronidases from the venom of the Asian hornet (Vespa velutina).";
RL   PLoS ONE 15:E0225672-E0225672(2020).
CC   -!- FUNCTION: Does not show toxicity when intravenously injected into mice
CC       tail. {ECO:0000269|PubMed:10484737}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31923175}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000269|PubMed:25896434}.
CC   -!- ALLERGEN: Causes an allergic reaction in human. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR   AlphaFoldDB; P0DMB9; -.
DR   SMR; P0DMB9; -.
DR   Allergome; 12286; Vesp v 5.
DR   Allergome; 12323; Vesp v 5.0101.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.40.33.10; -; 1.
DR   InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR   InterPro; IPR014044; CAP_domain.
DR   InterPro; IPR035940; CAP_sf.
DR   InterPro; IPR001283; CRISP-related.
DR   InterPro; IPR002413; V5_allergen-like.
DR   PANTHER; PTHR10334; PTHR10334; 1.
DR   Pfam; PF00188; CAP; 1.
DR   PRINTS; PR00838; V5ALLERGEN.
DR   PRINTS; PR00837; V5TPXLIKE.
DR   SMART; SM00198; SCP; 1.
DR   SUPFAM; SSF55797; SSF55797; 1.
PE   1: Evidence at protein level;
KW   Allergen; Direct protein sequencing; Disulfide bond; Glycation;
KW   Glycoprotein; Phosphoprotein; Secreted.
FT   CHAIN           1..202
FT                   /note="Venom allergen 5"
FT                   /id="PRO_0000425199"
FT   MOD_RES         107
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:P86686"
FT   CARBOHYD        138
FT                   /note="N-linked (Glc) (glycation) lysine"
FT                   /evidence="ECO:0000250|UniProtKB:P86686"
FT   DISULFID        4..16
FT                   /evidence="ECO:0000250|UniProtKB:Q05110"
FT   DISULFID        8..101
FT                   /evidence="ECO:0000250|UniProtKB:Q05110"
FT   DISULFID        26..94
FT                   /evidence="ECO:0000250|UniProtKB:Q05110"
FT   DISULFID        168..185
FT                   /evidence="ECO:0000250|UniProtKB:Q05110"
FT   CONFLICT        22
FT                   /note="T -> S (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   202 AA;  22718 MW;  23102A02647285D4 CRC64;
     NNYCKIKCRS GIHTLCKYGT STKPNCGRSV VKASGLTKAE KLEILKQHNE FRQKVARGLE
     TRGNPGPQPP AKSMNTLVWN DELAQIAQVW ASQCKYGHDN CRNTAKYLVG QNIAEQSTTA
     ASFEPVSNMV KMWSDEVKDY QYGSSKNKLN DVGHYTQMVW AKTKEIGCGN IKYIENGWHH
     HYLVCNYGPA GNIGNEPIYE KK