ID   VA5_VESVU               Reviewed;         227 AA.
AC   Q05110; Q9UB91;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Venom allergen 5;
DE   AltName: Full=Allergen Ves v V;
DE   AltName: Full=Antigen 5;
DE            Short=Ag5;
DE   AltName: Full=Cysteine-rich venom protein;
DE            Short=CRVP;
DE   AltName: Allergen=Ves v 5;
DE   Flags: Precursor;
OS   Vespula vulgaris (Yellow jacket) (Wasp).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Vespoidea;
OC   Vespidae; Vespinae; Vespula.
OX   NCBI_TaxID=7454;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=8454859;
RA   Lu G., Villalba M., Coscia M.R., Hoffman D.R., King T.P.;
RT   "Sequence analysis and antigenic cross-reactivity of a venom allergen,
RT   antigen 5, from hornets, wasps, and yellow jackets.";
RL   J. Immunol. 150:2823-2830(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-227.
RC   TISSUE=Venom gland;
RA   Suck R., Hagen S., Fiebig H.;
RT   "Molecular cloning of a genomic sequence from the venom allergen antigen 5
RT   from Vespula vulgaris.";
RL   Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 24-227, AND DISULFIDE BONDS.
RX   PubMed=11746691; DOI=10.1002/prot.1160;
RA   Henriksen A., King T.P., Mirza O., Monsalve R.I., Meno K., Ipsen H.,
RA   Larsen J.N., Gajhede M., Spangfort M.D.;
RT   "Major venom allergen of yellow jackets, Ves v 5: structural
RT   characterization of a pathogenesis-related protein superfamily.";
RL   Proteins 45:438-448(2001).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- ALLERGEN: Causes an allergic reaction in human.
CC   -!- SIMILARITY: Belongs to the CRISP family. Venom allergen 5-like
CC       subfamily. {ECO:0000305}.
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DR   EMBL; M98858; AAA30333.1; -; mRNA.
DR   EMBL; AJ238849; CAB42887.1; -; Genomic_DNA.
DR   PDB; 1QNX; X-ray; 1.90 A; A=24-227.
DR   PDBsum; 1QNX; -.
DR   AlphaFoldDB; Q05110; -.
DR   SMR; Q05110; -.
DR   Allergome; 3523; Ves v 5.0101.
DR   Allergome; 670; Ves v 5.
DR   EvolutionaryTrace; Q05110; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.40.33.10; -; 1.
DR   InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR   InterPro; IPR014044; CAP_domain.
DR   InterPro; IPR035940; CAP_sf.
DR   InterPro; IPR001283; CRISP-related.
DR   InterPro; IPR002413; V5_allergen-like.
DR   PANTHER; PTHR10334; PTHR10334; 1.
DR   Pfam; PF00188; CAP; 1.
DR   PRINTS; PR00838; V5ALLERGEN.
DR   PRINTS; PR00837; V5TPXLIKE.
DR   SMART; SM00198; SCP; 1.
DR   SUPFAM; SSF55797; SSF55797; 1.
DR   PROSITE; PS01009; CRISP_1; 1.
DR   PROSITE; PS01010; CRISP_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Allergen; Direct protein sequencing; Disulfide bond;
KW   Secreted; Signal.
FT   SIGNAL          1..23
FT   CHAIN           24..227
FT                   /note="Venom allergen 5"
FT                   /id="PRO_0000006297"
FT   DOMAIN          68..212
FT                   /note="SCP"
FT   DISULFID        27..40
FT                   /evidence="ECO:0000269|PubMed:11746691"
FT   DISULFID        31..124
FT                   /evidence="ECO:0000269|PubMed:11746691"
FT   DISULFID        49..117
FT                   /evidence="ECO:0000269|PubMed:11746691"
FT   DISULFID        193..210
FT                   /evidence="ECO:0000269|PubMed:11746691"
FT   CONFLICT        109
FT                   /note="V -> I (in Ref. 2; CAB42887)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        118
FT                   /note="Q -> E (in Ref. 2; CAB42887)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        173
FT                   /note="D -> N (in Ref. 2; CAB42887)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        219
FT                   /note="M -> K (in Ref. 2; CAB42887)"
FT                   /evidence="ECO:0000305"
FT   HELIX           26..28
FT                   /evidence="ECO:0007829|PDB:1QNX"
FT   HELIX           38..41
FT                   /evidence="ECO:0007829|PDB:1QNX"
FT   STRAND          53..58
FT                   /evidence="ECO:0007829|PDB:1QNX"
FT   HELIX           61..79
FT                   /evidence="ECO:0007829|PDB:1QNX"
FT   STRAND          94..96
FT                   /evidence="ECO:0007829|PDB:1QNX"
FT   HELIX           104..114
FT                   /evidence="ECO:0007829|PDB:1QNX"
FT   STRAND          128..130
FT                   /evidence="ECO:0007829|PDB:1QNX"
FT   STRAND          133..144
FT                   /evidence="ECO:0007829|PDB:1QNX"
FT   HELIX           149..157
FT                   /evidence="ECO:0007829|PDB:1QNX"
FT   HELIX           158..162
FT                   /evidence="ECO:0007829|PDB:1QNX"
FT   HELIX           169..171
FT                   /evidence="ECO:0007829|PDB:1QNX"
FT   HELIX           174..183
FT                   /evidence="ECO:0007829|PDB:1QNX"
FT   STRAND          190..200
FT                   /evidence="ECO:0007829|PDB:1QNX"
FT   STRAND          203..214
FT                   /evidence="ECO:0007829|PDB:1QNX"
SQ   SEQUENCE   227 AA;  25798 MW;  99E9813740A66F55 CRC64;
     MEISGLVYLI IIVTIIDLPY GKANNYCKIK CLKGGVHTAC KYGSLKPNCG NKVVVSYGLT
     KQEKQDILKE HNDFRQKIAR GLETRGNPGP QPPAKNMKNL VWNDELAYVA QVWANQCQYG
     HDTCRDVAKY QVGQNVALTG STAAKYDDPV KLVKMWEDEV KDYNPKKKFS GNDFLKTGHY
     TQMVWANTKE VGCGSIKYIQ EKWHKHYLVC NYGPSGNFMN EELYQTK