VA722_ARATH
ID VA722_ARATH Reviewed; 221 AA.
AC P47192; O49321;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Vesicle-associated membrane protein 722 {ECO:0000303|PubMed:11115874};
DE Short=AtVAMP722 {ECO:0000303|PubMed:11115874};
DE AltName: Full=Synaptobrevin-related protein 1 {ECO:0000303|Ref.1};
GN Name=VAMP722 {ECO:0000303|PubMed:11115874};
GN Synonyms=HAT24 {ECO:0000303|PubMed:1349174}, SAR1 {ECO:0000303|Ref.1};
GN OrderedLocusNames=At2g33120 {ECO:0000312|Araport:AT2G33120};
GN ORFNames=F25I18.14 {ECO:0000312|EMBL:AAC04921.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Schena M., Davis R.W.;
RT "The SAR1 gene of Arabidopsis thaliana encodes a member of synaptobrevin
RT family of membrane proteins.";
RL Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 140-200.
RC STRAIN=cv. Columbia;
RX PubMed=1349174; DOI=10.1073/pnas.89.9.3894;
RA Schena M., Davis R.W.;
RT "HD-Zip proteins: members of an Arabidopsis homeodomain protein
RT superfamily.";
RL Proc. Natl. Acad. Sci. U.S.A. 89:3894-3898(1992).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11115874; DOI=10.1104/pp.124.4.1558;
RA Sanderfoot A.A., Assaad F.F., Raikhel N.V.;
RT "The Arabidopsis genome. An abundance of soluble N-ethylmaleimide-sensitive
RT factor adaptor protein receptors.";
RL Plant Physiol. 124:1558-1569(2000).
RN [8]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=15342965; DOI=10.1247/csf.29.49;
RA Uemura T., Ueda T., Ohniwa R.L., Nakano A., Takeyasu K., Sato M.H.;
RT "Systematic analysis of SNARE molecules in Arabidopsis: dissection of the
RT post-Golgi network in plant cells.";
RL Cell Struct. Funct. 29:49-65(2004).
CC -!- FUNCTION: Involved in the targeting and/or fusion of transport vesicles
CC to their target membrane. {ECO:0000305|PubMed:11115874}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15342965};
CC Single-pass type IV membrane protein {ECO:0000250|UniProtKB:Q12255}.
CC Early endosome membrane {ECO:0000269|PubMed:15342965}; Single-pass type
CC IV membrane protein {ECO:0000250|UniProtKB:Q12255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=P47192-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Highly expressed in stems and roots. Detected in
CC flowers and leaves. {ECO:0000269|PubMed:15342965}.
CC -!- SIMILARITY: Belongs to the synaptobrevin family. {ECO:0000305}.
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DR EMBL; M90418; AAA56991.1; -; mRNA.
DR EMBL; AC002334; AAC04921.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08787.1; -; Genomic_DNA.
DR EMBL; AF419564; AAL31896.1; -; mRNA.
DR EMBL; AY072422; AAL62414.1; -; mRNA.
DR EMBL; AY079037; AAL79587.1; -; mRNA.
DR EMBL; AY114706; AAM48025.1; -; mRNA.
DR EMBL; AY128288; AAM91096.1; -; mRNA.
DR EMBL; AY086363; AAM64431.1; -; mRNA.
DR PIR; D44088; D44088.
DR PIR; F84741; F84741.
DR RefSeq; NP_180871.1; NM_128872.3. [P47192-1]
DR AlphaFoldDB; P47192; -.
DR SMR; P47192; -.
DR BioGRID; 3221; 21.
DR DIP; DIP-59828N; -.
DR IntAct; P47192; 13.
DR STRING; 3702.AT2G33120.2; -.
DR PaxDb; P47192; -.
DR PRIDE; P47192; -.
DR ProteomicsDB; 242306; -. [P47192-1]
DR EnsemblPlants; AT2G33120.1; AT2G33120.1; AT2G33120. [P47192-1]
DR GeneID; 817874; -.
DR Gramene; AT2G33120.1; AT2G33120.1; AT2G33120. [P47192-1]
DR KEGG; ath:AT2G33120; -.
DR Araport; AT2G33120; -.
DR eggNOG; KOG0859; Eukaryota.
DR HOGENOM; CLU_064620_1_0_1; -.
DR InParanoid; P47192; -.
DR OMA; IMRDTEE; -.
DR PhylomeDB; P47192; -.
DR PRO; PR:P47192; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; P47192; baseline and differential.
DR Genevisible; P47192; AT.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR CDD; cd14824; Longin; 1.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR InterPro; IPR010908; Longin_dom.
DR InterPro; IPR001388; Synaptobrevin.
DR InterPro; IPR042855; V_SNARE_CC.
DR Pfam; PF13774; Longin; 1.
DR Pfam; PF00957; Synaptobrevin; 1.
DR PRINTS; PR00219; SYNAPTOBREVN.
DR SMART; SM01270; Longin; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
DR PROSITE; PS50859; LONGIN; 1.
DR PROSITE; PS00417; SYNAPTOBREVIN; 1.
DR PROSITE; PS50892; V_SNARE; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Coiled coil; Endosome; Membrane;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..221
FT /note="Vesicle-associated membrane protein 722"
FT /id="PRO_0000206755"
FT TOPO_DOM 1..196
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 218..221
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT DOMAIN 10..114
FT /note="Longin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00231"
FT DOMAIN 130..190
FT /note="v-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00290"
FT CONFLICT 123
FT /note="D -> A (in Ref. 1; AAA56991)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 221 AA; 24928 MW; 880AA7BA2C24697B CRC64;
MAQQSLIYSF VARGTVILVE FTDFKGNFTS IAAQCLQKLP SSNNKFTYNC DGHTFNYLVE
NGFTYCVVAV DSAGRQIPMA FLERVKEDFN KRYGGGKAAT AQANSLNKEF GSKLKEHMQY
CMDHPDEISK LAKVKAQVSE VKGVMMENIE KVLDRGEKIE LLVDKTENLR SQAQDFRTQG
TQMRRKMWFQ NMKIKLIVLA IIIALILIII LSICGGFNCG K
