VA725_ARATH
ID VA725_ARATH Reviewed; 285 AA.
AC O48850; Q8GXC1;
DT 17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 2.
DT 25-MAY-2022, entry version 148.
DE RecName: Full=Vesicle-associated membrane protein 725 {ECO:0000303|PubMed:11115874};
DE Short=AtVAMP725 {ECO:0000303|PubMed:11115874};
GN Name=VAMP725 {ECO:0000303|PubMed:11115874};
GN OrderedLocusNames=At2g32670 {ECO:0000312|Araport:AT2G32670};
GN ORFNames=F24L7.19 {ECO:0000312|EMBL:AAC04496.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11115874; DOI=10.1104/pp.124.4.1558;
RA Sanderfoot A.A., Assaad F.F., Raikhel N.V.;
RT "The Arabidopsis genome. An abundance of soluble N-ethylmaleimide-sensitive
RT factor adaptor protein receptors.";
RL Plant Physiol. 124:1558-1569(2000).
RN [6]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=15342965; DOI=10.1247/csf.29.49;
RA Uemura T., Ueda T., Ohniwa R.L., Nakano A., Takeyasu K., Sato M.H.;
RT "Systematic analysis of SNARE molecules in Arabidopsis: dissection of the
RT post-Golgi network in plant cells.";
RL Cell Struct. Funct. 29:49-65(2004).
RN [7]
RP 3D-STRUCTURE MODELING.
RX PubMed=19889231; DOI=10.1186/1471-2164-10-510;
RA Vedovato M., Rossi V., Dacks J.B., Filippini F.;
RT "Comparative analysis of plant genomes allows the definition of the
RT 'Phytolongins': a novel non-SNARE longin domain protein family.";
RL BMC Genomics 10:510-510(2009).
CC -!- FUNCTION: Involved in the targeting and/or fusion of transport vesicles
CC to their target membrane. {ECO:0000305|PubMed:11115874}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15342965};
CC Single-pass type IV membrane protein {ECO:0000250|UniProtKB:Q12255}.
CC Early endosome membrane {ECO:0000269|PubMed:15342965}; Single-pass type
CC IV membrane protein {ECO:0000250|UniProtKB:Q12255}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers, leaves, stems and roots.
CC {ECO:0000269|PubMed:15342965}.
CC -!- SIMILARITY: Belongs to the synaptobrevin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC04496.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AC003974; AAC04496.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002685; AEC08714.1; -; Genomic_DNA.
DR EMBL; AK118318; BAC42934.1; -; mRNA.
DR EMBL; BT006042; AAP06822.1; -; mRNA.
DR PIR; T00801; T00801.
DR RefSeq; NP_180826.2; NM_128826.4.
DR AlphaFoldDB; O48850; -.
DR SMR; O48850; -.
DR BioGRID; 3174; 1.
DR STRING; 3702.AT2G32670.1; -.
DR PaxDb; O48850; -.
DR PRIDE; O48850; -.
DR ProteomicsDB; 243255; -.
DR EnsemblPlants; AT2G32670.1; AT2G32670.1; AT2G32670.
DR GeneID; 817827; -.
DR Gramene; AT2G32670.1; AT2G32670.1; AT2G32670.
DR KEGG; ath:AT2G32670; -.
DR Araport; AT2G32670; -.
DR TAIR; locus:2046367; AT2G32670.
DR eggNOG; KOG0859; Eukaryota.
DR HOGENOM; CLU_064620_1_0_1; -.
DR InParanoid; O48850; -.
DR OMA; HSQAQGF; -.
DR OrthoDB; 1211929at2759; -.
DR PhylomeDB; O48850; -.
DR PRO; PR:O48850; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O48850; baseline and differential.
DR Genevisible; O48850; AT.
DR GO; GO:0031901; C:early endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005768; C:endosome; TAS:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; TAS:TAIR.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR CDD; cd14824; Longin; 1.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR InterPro; IPR010908; Longin_dom.
DR InterPro; IPR001388; Synaptobrevin.
DR InterPro; IPR042855; V_SNARE_CC.
DR Pfam; PF13774; Longin; 1.
DR Pfam; PF00957; Synaptobrevin; 1.
DR PRINTS; PR00219; SYNAPTOBREVN.
DR SMART; SM01270; Longin; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
DR PROSITE; PS50859; LONGIN; 1.
DR PROSITE; PS00417; SYNAPTOBREVIN; 1.
DR PROSITE; PS50892; V_SNARE; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Coiled coil; Endosome; Membrane; Protein transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..285
FT /note="Vesicle-associated membrane protein 725"
FT /id="PRO_0000206758"
FT TOPO_DOM 1..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 262..282
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 283..285
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT DOMAIN 75..179
FT /note="Longin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00231"
FT DOMAIN 195..255
FT /note="v-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00290"
SQ SEQUENCE 285 AA; 32691 MW; F8A28595547166EE CRC64;
MDRSVVPISL APFQFLLVFW IFLTSVHTNP NKQQKTVVSL SLWWNSKNRI RGCVWFFFLL
RVTRTMGQQN LIYSFVARGT VILVEYTEFK GNFTAVAAQC LQKLPSSNNK FTYNCDGHTF
NYLVENGFTY CVVAVESVGR QIPMAFLERV KEDFNKRYGG GKATTAQANS LNREFGSKLK
EHMQYCVDHP DEISKLAKVK AQVTEVKGVM MENIEKVLDR GEKIELLVDK TENLRSQAQD
FRTQGTKIRR KMWFENMKIK LIVLGIIITL ILIIILSVCG GFKCT
