VAB2_YEAST
ID VAB2_YEAST Reviewed; 282 AA.
AC P40003; D3DLP2;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Biogenesis of lysosome-related organelles complex 1 subunit VAB2;
DE Short=BLOC-1 subunit VAB2;
DE AltName: Full=VAC8-binding protein 2;
GN Name=VAB2; Synonyms=VAB31; OrderedLocusNames=YEL005C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP INTERACTION WITH VAC8.
RX PubMed=10888680; DOI=10.1091/mbc.11.7.2445;
RA Pan X., Roberts P., Chen Y., Kvam E., Shulga N., Huang K., Lemmon S.,
RA Goldfarb D.S.;
RT "Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through
RT the direct interaction of Vac8p with Nvj1p.";
RL Mol. Biol. Cell 11:2445-2457(2000).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP IDENTIFICATION IN THE BLOC-1 COMPLEX, AND FUNCTION.
RX PubMed=21159114; DOI=10.1111/j.1600-0854.2010.01151.x;
RA Hayes M.J., Bryon K., Satkurunathan J., Levine T.P.;
RT "Yeast homologues of three BLOC-1 subunits highlight KxDL proteins as
RT conserved interactors of BLOC-1.";
RL Traffic 12:260-268(2011).
CC -!- FUNCTION: Component of the biogenesis of lysosome-related organelles
CC complex-1 (BLOC-1) involved in endosomal cargo sorting.
CC {ECO:0000269|PubMed:21159114}.
CC -!- SUBUNIT: Component of the biogenesis of lysosome-related organelles
CC complex-1 (BLOC-1) composed of at least BLI1, BLS1, CNL1, KXD1, SNN1
CC and VAB2. Interacts with VAC8. {ECO:0000269|PubMed:10888680,
CC ECO:0000269|PubMed:21159114}.
CC -!- INTERACTION:
CC P40003; P48232: SNN1; NbExp=4; IntAct=EBI-22275, EBI-28775;
CC P40003; P39968: VAC8; NbExp=3; IntAct=EBI-22275, EBI-20212;
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle
CC {ECO:0000269|PubMed:14562095}. Cytoplasm {ECO:0000269|PubMed:14562095}.
CC Vacuole {ECO:0000269|PubMed:14562095}.
CC -!- SIMILARITY: Belongs to the VAB2 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U18530; AAB64482.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07646.1; -; Genomic_DNA.
DR PIR; S50454; S50454.
DR RefSeq; NP_010911.3; NM_001178820.3.
DR AlphaFoldDB; P40003; -.
DR SMR; P40003; -.
DR BioGRID; 36726; 61.
DR ComplexPortal; CPX-1153; BLOC-1 complex.
DR DIP; DIP-5277N; -.
DR IntAct; P40003; 11.
DR MINT; P40003; -.
DR STRING; 4932.YEL005C; -.
DR iPTMnet; P40003; -.
DR MaxQB; P40003; -.
DR PaxDb; P40003; -.
DR PRIDE; P40003; -.
DR EnsemblFungi; YEL005C_mRNA; YEL005C; YEL005C.
DR GeneID; 856713; -.
DR KEGG; sce:YEL005C; -.
DR SGD; S000000731; VAB2.
DR VEuPathDB; FungiDB:YEL005C; -.
DR eggNOG; ENOG502S95F; Eukaryota.
DR HOGENOM; CLU_086126_0_0_1; -.
DR InParanoid; P40003; -.
DR OMA; HICITEP; -.
DR BioCyc; YEAST:G3O-30134-MON; -.
DR PRO; PR:P40003; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P40003; protein.
DR GO; GO:0031083; C:BLOC-1 complex; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005768; C:endosome; IDA:ComplexPortal.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0007032; P:endosome organization; IMP:SGD.
DR GO; GO:0032880; P:regulation of protein localization; IMP:SGD.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Cytoplasmic vesicle; Reference proteome; Transport;
KW Vacuole.
FT CHAIN 1..282
FT /note="Biogenesis of lysosome-related organelles complex 1
FT subunit VAB2"
FT /id="PRO_0000202618"
FT COILED 64..140
FT /evidence="ECO:0000255"
SQ SEQUENCE 282 AA; 31365 MW; 8F7CA122F91E491A CRC64;
MVADLTKGIL KWKSRIEFDA VGSSSYYEEL KGLPPLASHK KLTQAAIFNS TKYELLQVKK
DILSIYEVVS RDIDEERNQM QQIELQLKKS LKKVEHSYKN VLKQRASTNC INGNDRLLAN
AEKKIGSLNE ELACVNDIVS DIVNNLTALN ANLPKKAQLL KDDSINVAHY PLLFDFLHKS
CPKSIIATSE ASIHENASPS PLLEHDELPA ESINSFYGEN ELQSDSLAPL QTHDDNISSC
KKILPPKFNT TSGPSIETNF ENISADGLTY TKCSLKNSIS LT
