VAB3_CAEEL
ID VAB3_CAEEL Reviewed; 455 AA.
AC G5EDS1; G5EE71; Q17356; Q95ZV2;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Paired box protein 6 homolog {ECO:0000305|PubMed:30890567};
DE AltName: Full=Homeobox and paired domain-containing protein vab-3 {ECO:0000305|PubMed:7659159};
DE AltName: Full=Protein male abnormal 18 {ECO:0000312|WormBase:F14F3.1a};
DE AltName: Full=Variable abnormal morphology protein 3 {ECO:0000312|WormBase:F14F3.1a};
GN Name=vab-3 {ECO:0000312|WormBase:F14F3.1a};
GN Synonyms=mab-18 {ECO:0000303|PubMed:1782863,
GN ECO:0000312|WormBase:F14F3.1a}, pax-6 {ECO:0000303|PubMed:7659159};
GN ORFNames=F14F3.1 {ECO:0000312|WormBase:F14F3.1a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:AAA82991.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE (ISOFORM A), AND MUTAGENESIS OF 101-TRP--GLN-455 AND
RP ILE-103.
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:AAA82991.1};
RX PubMed=7659159; DOI=10.1038/377052a0;
RA Chisholm A.D., Horvitz H.R.;
RT "Patterning of the Caenorhabditis elegans head region by the Pax-6 family
RT member vab-3.";
RL Nature 376:52-55(1995).
RN [2] {ECO:0000312|EMBL:AAC47541.1, ECO:0000312|EMBL:AAC47542.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND C), FUNCTION, SUBCELLULAR
RP LOCATION, AND DEVELOPMENTAL STAGE (ISOFORM B).
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:AAC47542.1};
RX PubMed=7659160; DOI=10.1038/377055a0;
RA Zhang Y., Emmons S.W.;
RT "Specification of sense-organ identity by a Caenorhabditis elegans Pax-6
RT homologue.";
RL Nature 377:55-59(1995).
RN [3] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4] {ECO:0000305}
RP FUNCTION (ISOFORM B).
RX PubMed=1782863; DOI=10.1242/dev.113.2.515;
RA Baird S.E., Fitch D.H., Kassem I.A., Emmons S.W.;
RT "Pattern formation in the nematode epidermis: determination of the
RT arrangement of peripheral sense organs in the C. elegans male tail.";
RL Development 113:515-526(1991).
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=7649393; DOI=10.1006/dbio.1995.1246;
RA Chamberlin H.M., Sternberg P.W.;
RT "Mutations in the Caenorhabditis elegans gene vab-3 reveal distinct roles
RT in fate specification and unequal cytokinesis in an asymmetric cell
RT division.";
RL Dev. Biol. 170:679-689(1995).
RN [6] {ECO:0000305}
RP FUNCTION (ISOFORM A), AND MUTAGENESIS OF 149-ARG--GLN-455 AND
RP 101-TRP--GLN-455.
RX PubMed=8647436; DOI=10.1101/gad.10.11.1395;
RA Baumeister R., Liu Y., Ruvkun G.;
RT "Lineage-specific regulators couple cell lineage asymmetry to the
RT transcription of the Caenorhabditis elegans POU gene unc-86 during
RT neurogenesis.";
RL Genes Dev. 10:1395-1410(1996).
RN [7] {ECO:0000305}
RP SUBCELLULAR LOCATION (ISOFORM B), AND DEVELOPMENTAL STAGE (ISOFORM B).
RX PubMed=9858725; DOI=10.1016/s0925-4773(98)00171-3;
RA Zhang Y., Ferreira H.B., Greenstein D., Chisholm A., Emmons S.W.;
RT "Regulated nuclear entry of the C. elegans Pax-6 transcription factor.";
RL Mech. Dev. 78:179-187(1998).
RN [8] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF 101-TRP--GLN-455 AND GLY-13.
RX PubMed=10388818; DOI=10.1093/genetics/152.3.985;
RA Nishiwaki K.;
RT "Mutations affecting symmetrical migration of distal tip cells in
RT Caenorhabditis elegans.";
RL Genetics 152:985-997(1999).
RN [9] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF 101-TRP--GLN-455.
RC TISSUE=Embryo;
RX PubMed=11476572; DOI=10.1006/dbio.2001.0325;
RA Dozier C., Kagoshima H., Niklaus G., Cassata G., Buerglin T.R.;
RT "The Caenorhabditis elegans Six/sine oculis class homeobox gene ceh-32 is
RT required for head morphogenesis.";
RL Dev. Biol. 236:289-303(2001).
RN [10] {ECO:0000305}
RP FUNCTION (ISOFORMS A AND B), AND MUTAGENESIS OF GLY-13; GLY-19; SER-75;
RP ILE-103; 101-TRP--GLN-455; 149-ARG--GLN-455; 228-GLN--GLN-455;
RP 229-ILE--LEU-232 AND 262-GLN--GLN-455.
RX PubMed=15579687; DOI=10.1534/genetics.104.031724;
RA Cinar H.N., Chisholm A.D.;
RT "Genetic analysis of the Caenorhabditis elegans pax-6 locus: roles of
RT paired domain-containing and nonpaired domain-containing isoforms.";
RL Genetics 168:1307-1322(2004).
RN [11] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP GLY-52; 101-TRP--GLN-455 AND 229-ILE--LEU-232.
RX PubMed=17606640; DOI=10.1101/gad.1534807;
RA Meighan C.M., Schwarzbauer J.E.;
RT "Control of C. elegans hermaphrodite gonad size and shape by vab-3/Pax6-
RT mediated regulation of integrin receptors.";
RL Genes Dev. 21:1615-1620(2007).
RN [12] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF GLY-19; SER-75; 101-TRP--GLN-455; SER-120;
RP 229-ILE--LEU-232 AND 262-GLN--GLN-455.
RX PubMed=18508862; DOI=10.1242/dev.019547;
RA Yoshimura S., Murray J.I., Lu Y., Waterston R.H., Shaham S.;
RT "mls-2 and vab-3 Control glia development, hlh-17/Olig expression and glia-
RT dependent neurite extension in C. elegans.";
RL Development 135:2263-2275(2008).
RN [13] {ECO:0000305}
RP DEVELOPMENTAL STAGE.
RX PubMed=18313275; DOI=10.1016/j.mod.2008.01.007;
RA Johnson R.W., Chamberlin H.M.;
RT "Positive and negative regulatory inputs restrict pax-6/vab-3 transcription
RT to sensory organ precursors in Caenorhabditis elegans.";
RL Mech. Dev. 125:486-497(2008).
RN [14] {ECO:0000305}
RP FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF 101-TRP--GLN-455;
RP 160-TRP--GLN-455 AND 338-GLN--GLN-455.
RX PubMed=21079745; DOI=10.1371/journal.pone.0015435;
RA Doitsidou M., Poole R.J., Sarin S., Bigelow H., Hobert O.;
RT "C. elegans mutant identification with a one-step whole-genome-sequencing
RT and SNP mapping strategy.";
RL PLoS ONE 5:e15435-e15435(2010).
RN [15] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP GLY-52; 160-TRP--GLN-455; 262-GLN--GLN-455 AND 338-GLN--GLN-455.
RX PubMed=30890567; DOI=10.1242/dev.168153;
RA Brandt J.P., Rossillo M., Du Z., Ichikawa D., Barnes K., Chen A., Noyes M.,
RA Bao Z., Ringstad N.;
RT "Lineage context switches the function of a C. elegans Pax6 homolog in
RT determining a neuronal fate.";
RL Development 146:0-0(2019).
CC -!- FUNCTION: Transcription factor that binds a motif with the core
CC sequence 5'-GCGTA-3' in the promoter of various genes (PubMed:11476572,
CC PubMed:30890567). During development, required for cell fate
CC specification probably by promoting or repressing expression of genes
CC involved in specific cell fate (PubMed:30890567, PubMed:11476572,
CC PubMed:7649393). Involved in head epidermal morphogenesis
CC (PubMed:7659159). Involved in gonadal distal tip cell (DTC) migration,
CC during which modulates expression of the integrin alpha genes, pat-2
CC and ina-1 (PubMed:10388818, PubMed:17606640, PubMed:15579687).
CC Regulates ventral and dorsal cephalic sheath (CEPsh) glia
CC differentiation and expression of transcription factor hlh-17 in CEPsh
CC glia (PubMed:18508862). Plays a role in establishing unequal
CC cytokinesis and cell fate specification in male-specific postembryonic
CC blast cell B (PubMed:7649393). May cooperate with the phosphatase eya-1
CC and transcription factor ceh-32 to regulate the transcription factor
CC ets-5 (PubMed:30890567). {ECO:0000269|PubMed:10388818,
CC ECO:0000269|PubMed:11476572, ECO:0000269|PubMed:15579687,
CC ECO:0000269|PubMed:17606640, ECO:0000269|PubMed:18508862,
CC ECO:0000269|PubMed:30890567, ECO:0000269|PubMed:7649393,
CC ECO:0000269|PubMed:7659159}.
CC -!- FUNCTION: [Isoform a]: Transcription factor involved in head epidermal
CC morphogenesis and required for normal cell fate in anterior lateral
CC epidermal blast (seam) cells (PubMed:7659159, PubMed:15579687).
CC Required for the generation or differentiation of neurons of the
CC anterior ganglion, probably acting upstream of unc-86 (PubMed:8647436).
CC Represses BAG sensory neuron fate in non-BAG cells, probably through
CC cooperation with the phosphatase eya-1 and transcription factor ceh-32
CC (PubMed:30890567). May be involved in regulating the generation of
CC dopaminergic neurons (PubMed:21079745). May cooperate with hlh-17 to
CC preferentially regulate expression of hlh-17 in ventral CEPsh glia
CC (PubMed:18508862). {ECO:0000269|PubMed:15579687,
CC ECO:0000269|PubMed:18508862, ECO:0000269|PubMed:21079745,
CC ECO:0000269|PubMed:30890567, ECO:0000269|PubMed:7659159,
CC ECO:0000269|PubMed:8647436}.
CC -!- FUNCTION: [Isoform b]: Transcription factor that acts within the male-
CC specific genital sensilla (simple sense organs) known as rays to
CC determine their identity (PubMed:7659160, PubMed:1782863,
CC PubMed:15579687). Promotes BAG sensory neuron fate in a cell-autonomous
CC manner (PubMed:30890567). Required for function of chemosensory BAG
CC neurons (PubMed:30890567). {ECO:0000269|PubMed:15579687,
CC ECO:0000269|PubMed:1782863, ECO:0000269|PubMed:30890567,
CC ECO:0000269|PubMed:7659160}.
CC -!- INTERACTION:
CC G5EDS1; Q10929: abi-1; NbExp=6; IntAct=EBI-319610, EBI-315750;
CC G5EDS1; P03949: abl-1; NbExp=4; IntAct=EBI-319610, EBI-2315883;
CC G5EDS1; Q9N4P3: CELE_ZK121.2; NbExp=3; IntAct=EBI-319610, EBI-317902;
CC G5EDS1; Q7K7J0: gei-18; NbExp=9; IntAct=EBI-319610, EBI-2315822;
CC G5EDS1; Q95XW5: magu-1; NbExp=5; IntAct=EBI-319610, EBI-2317194;
CC G5EDS1; G5EC32: sorb-1; NbExp=14; IntAct=EBI-319610, EBI-325337;
CC G5EDS1; Q8MPT2: T04C9.1; NbExp=7; IntAct=EBI-319610, EBI-2315635;
CC G5EDS1; G5EGG0: uso-1; NbExp=3; IntAct=EBI-319610, EBI-311986;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|RuleBase:RU000682, ECO:0000269|PubMed:7659159}.
CC -!- SUBCELLULAR LOCATION: [Isoform b]: Nucleus {ECO:0000269|PubMed:7659160,
CC ECO:0000269|PubMed:9858725}. Cytoplasm {ECO:0000269|PubMed:9858725}.
CC Note=Nuclear localization is temporally and spatially regulated in two
CC sets of neuronal sublineages. {ECO:0000269|PubMed:9858725}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=a {ECO:0000312|WormBase:F14F3.1a};
CC IsoId=G5EDS1-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:F14F3.1b};
CC IsoId=G5EDS1-2; Sequence=VSP_060800;
CC Name=c {ECO:0000312|WormBase:F14F3.1c};
CC IsoId=G5EDS1-3; Sequence=VSP_060801;
CC -!- TISSUE SPECIFICITY: Expressed widely in head epidermal cells and
CC neurons, including BAG sensory neurons. {ECO:0000269|PubMed:30890567}.
CC -!- TISSUE SPECIFICITY: [Isoform a]: Not expressed in BAG neurons.
CC {ECO:0000269|PubMed:30890567}.
CC -!- TISSUE SPECIFICITY: [Isoform b]: Expressed in BAG neurons.
CC {ECO:0000269|PubMed:30890567}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the anterior part of comma-stage
CC embryos (PubMed:11476572, PubMed:18313275). In larvae, expressed
CC strongly in almost all anterior hypodermal cells and weakly in many
CC head neurons (PubMed:7659159, PubMed:18313275). Expressed in the
CC gonadal DTCs during ventral migration in hermaphrodite L4 larvae
CC (PubMed:17606640, PubMed:18313275). Expressed in the male hindgut, in
CC B.a and Y.p sensory organ precursors, from the early L2 and until the
CC mid L3 larval stages (PubMed:18313275). Expression diminishes in the
CC B.a lineage during late L4 tail morphogenesis, and is absent in adults
CC (PubMed:18313275). Expression in the Y.p lineage diminishes in the mid
CC L3 stage (PubMed:18313275). Male hindgut expression is restricted to
CC specific lineages in a lin-48 and egl-38 dependent manner
CC (PubMed:18313275). {ECO:0000269|PubMed:11476572,
CC ECO:0000269|PubMed:17606640, ECO:0000269|PubMed:18313275,
CC ECO:0000269|PubMed:7659159}.
CC -!- DEVELOPMENTAL STAGE: [Isoform a]: First expressed at about 5 hours of
CC embryogenesis in 20-30 anterior dorsal cells, corresponding to the
CC hypodermal cells of the head, and lateral sets of about 10 cells, of
CC which some are probably future head neurons (PubMed:7659159). By the
CC 1.5-fold stage, expressed in hypodermal and neuronal cells in a broad
CC region of the head (PubMed:7659159). {ECO:0000269|PubMed:7659159}.
CC -!- DEVELOPMENTAL STAGE: [Isoform b]: Expressed in RS.a and RB.p in late
CC larval stage L3, in ray 6 and ray 8 cells in late L4, in neurons
CC descended from P11.a in the preanal ganglion in L4 males and head
CC hypodermis and a pair of bilaterally symmetrical neurons in the head
CC anterior of the nerve ring in L4 males (at protein level)
CC (PubMed:7659160, PubMed:9858725). Also expressed in seminal vesicle
CC cells in late L4 males (at protein level) (PubMed:9858725).
CC {ECO:0000269|PubMed:7659160, ECO:0000269|PubMed:9858725}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes defects in gonadal
CC distal tip cell migration (PubMed:17606640). RNAi-mediated knockdown
CC reduces expression of the integrin alpha pat-2 in gonadal DTCs in about
CC 70% of individuals and in 90% on a vab-3 mutant background
CC (PubMed:17606640). RNAi-mediated knockdown increases expression of the
CC integrin alpha ina-1 in gonadal DTCs in hermaphrodites
CC (PubMed:17606640). RNAi-mediated knockdown induces generation of
CC additional dopaminergic neurons (PubMed:21079745). BAG sensory neuron-
CC specific RNAi-mediated knockdown reduces expression of receptor-type
CC guanylyl cyclase gcy-9 (PubMed:30890567). {ECO:0000269|PubMed:17606640,
CC ECO:0000269|PubMed:21079745, ECO:0000269|PubMed:30890567}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC47541.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
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DR EMBL; U31537; AAA82991.1; -; mRNA.
DR EMBL; U29145; AAC47541.1; ALT_SEQ; mRNA.
DR EMBL; U29184; AAC47542.1; -; mRNA.
DR EMBL; BX284606; CAA90186.2; -; Genomic_DNA.
DR EMBL; BX284606; CAC42287.1; -; Genomic_DNA.
DR EMBL; BX284606; CAC42288.1; -; Genomic_DNA.
DR PIR; S60250; S60250.
DR PIR; S60251; S60251.
DR PIR; T20900; S60252.
DR RefSeq; NP_001024570.1; NM_001029399.2. [G5EDS1-1]
DR RefSeq; NP_001024571.1; NM_001029400.1. [G5EDS1-2]
DR RefSeq; NP_001024572.1; NM_001029401.3. [G5EDS1-3]
DR AlphaFoldDB; G5EDS1; -.
DR SMR; G5EDS1; -.
DR IntAct; G5EDS1; 80.
DR STRING; 6239.F14F3.1a; -.
DR EPD; G5EDS1; -.
DR PaxDb; G5EDS1; -.
DR PeptideAtlas; G5EDS1; -.
DR EnsemblMetazoa; F14F3.1a.1; F14F3.1a.1; WBGene00006870. [G5EDS1-1]
DR EnsemblMetazoa; F14F3.1a.2; F14F3.1a.2; WBGene00006870. [G5EDS1-1]
DR EnsemblMetazoa; F14F3.1b.1; F14F3.1b.1; WBGene00006870. [G5EDS1-2]
DR EnsemblMetazoa; F14F3.1c.1; F14F3.1c.1; WBGene00006870. [G5EDS1-3]
DR GeneID; 181251; -.
DR KEGG; cel:CELE_F14F3.1; -.
DR CTD; 181251; -.
DR WormBase; F14F3.1a; CE24899; WBGene00006870; vab-3.
DR WormBase; F14F3.1b; CE28216; WBGene00006870; vab-3.
DR WormBase; F14F3.1c; CE28217; WBGene00006870; vab-3.
DR eggNOG; KOG0849; Eukaryota.
DR GeneTree; ENSGT00940000165785; -.
DR HOGENOM; CLU_019281_5_0_1; -.
DR InParanoid; G5EDS1; -.
DR OMA; MSDAGHT; -.
DR OrthoDB; 1152885at2759; -.
DR PhylomeDB; G5EDS1; -.
DR SignaLink; G5EDS1; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00006870; Expressed in pharyngeal muscle cell (C elegans) and 6 other tissues.
DR ExpressionAtlas; G5EDS1; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048856; P:anatomical structure development; IBA:GO_Central.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:WormBase.
DR GO; GO:0045165; P:cell fate commitment; IMP:WormBase.
DR GO; GO:1903355; P:negative regulation of distal tip cell migration; IMP:UniProtKB.
DR GO; GO:0090597; P:nematode male tail mating organ morphogenesis; IMP:UniProtKB.
DR GO; GO:0045138; P:nematode male tail tip morphogenesis; IMP:WormBase.
DR GO; GO:0045687; P:positive regulation of glial cell differentiation; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0030155; P:regulation of cell adhesion; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISS:WormBase.
DR CDD; cd00086; homeodomain; 1.
DR CDD; cd00131; PAX; 1.
DR Gene3D; 1.10.10.10; -; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR043182; PAIRED_DNA-bd_dom.
DR InterPro; IPR001523; Paired_dom.
DR InterPro; IPR043565; PAX_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR45636; PTHR45636; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00292; PAX; 1.
DR PRINTS; PR00027; PAIREDBOX.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00351; PAX; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00034; PAIRED_1; 1.
DR PROSITE; PS51057; PAIRED_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; DNA-binding;
KW Homeobox; Nucleus; Paired box; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..455
FT /note="Paired box protein 6 homolog"
FT /id="PRO_0000451521"
FT DNA_BIND 5..131
FT /note="Paired"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT DNA_BIND 217..276
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 8..64
FT /note="PAI subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 83..131
FT /note="RED subdomain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00381"
FT REGION 274..330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 284..330
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..194
FT /note="MSDAGHTGVNQLGGVFVNGRPLPDATRQRIVDLAHKGCRPCDISRLLQVSNG
FT CVSKILCRYYESGTIRPRAIGGSKPRVATSDVVEKIEDYKRDQPSIFAWEIRDKLLADN
FT ICNNETIPSVSSINRVLRNLAAKKEQVTMQTELYDRIRIVDNFPYNSSWYGQWPIPMNG
FT AVGLNPFVPAPLIEPKTEGEFEKD -> MRSIANTN (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060800"
FT VAR_SEQ 1..194
FT /note="MSDAGHTGVNQLGGVFVNGRPLPDATRQRIVDLAHKGCRPCDISRLLQVSNG
FT CVSKILCRYYESGTIRPRAIGGSKPRVATSDVVEKIEDYKRDQPSIFAWEIRDKLLADN
FT ICNNETIPSVSSINRVLRNLAAKKEQVTMQTELYDRIRIVDNFPYNSSWYGQWPIPMNG
FT AVGLNPFVPAPLIEPKTEGEFEKD -> MISAHAPIVSIKAPLPTRRASTIRKTARVGK
FT FSPY (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_060801"
FT MUTAGEN 13
FT /note="G->Q: In k143; embryonic lethality in a few mutants.
FT Anterior and posterior defects in gonadal distal tip cell
FT (DTC) migration. No defect in head morphogenesis."
FT /evidence="ECO:0000269|PubMed:10388818,
FT ECO:0000269|PubMed:15579687"
FT MUTAGEN 19
FT /note="G->R: In sy281; defects in head morphogenesis,
FT resulting from defects in the embryonic migration or
FT determination of head hypodermal cells. Male tail spicule
FT defects. Reduces expression of hlh-17 and ptr-10 in both
FT dorsal and ventral CEPsh glia."
FT /evidence="ECO:0000269|PubMed:15579687,
FT ECO:0000269|PubMed:18508862"
FT MUTAGEN 52
FT /note="G->E: In mw105; defects in gonadal distal tip cell
FT migration in larval development. About 50% of individuals
FT have reduced expression of the integrin alpha pat-2.
FT Hermaphrodites express integrin alpha ina-1 abnormally,
FT beyond the egg-laying stage."
FT /evidence="ECO:0000269|PubMed:17606640"
FT MUTAGEN 52
FT /note="G->R: In wz25; abnormal BAG sensory neuron fate and
FT inappropriate expression of the transcription factor ets-5
FT in non-BAG cells."
FT /evidence="ECO:0000269|PubMed:30890567"
FT MUTAGEN 75
FT /note="S->L: In k109; embryonic or larval lethality in a
FT few mutants. Male tail spicule defects. Almost abolishes
FT expression of hlh-17 and ptr-10 in dorsal CEPsh glia,
FT whereas nearly half of animals express both these genes in
FT ventral CEPsh glia. No defect in head morphogenesis."
FT /evidence="ECO:0000269|PubMed:15579687,
FT ECO:0000269|PubMed:18508862"
FT MUTAGEN 101..455
FT /note="Missing: In e648; lethality at embryonic or larval
FT stages. The few surviving adults have defects in head
FT morphogenesis, resulting from defects in the embryonic
FT migration or determination of head hypodermal cells. Male
FT tail spicule defects. Defective dopaminergic neuron
FT specification. Expression of ceh-32 is abolished in some
FT head hypodermal cells. Anatomy of the anterior ganglion is
FT severely disturbed and expression of unc-86 is abolished in
FT several neurons in the head. Defects in gonadal distal tip
FT cell migration in larval development. Abnormal BAG sensory
FT neuron fate. Defects in male specific postembryonic blast
FT cell (B cell) fate specification. Expression of hlh-17
FT abolished in both dorsal and ventral CEPsh glia."
FT /evidence="ECO:0000269|PubMed:10388818,
FT ECO:0000269|PubMed:11476572, ECO:0000269|PubMed:15579687,
FT ECO:0000269|PubMed:17606640, ECO:0000269|PubMed:18508862,
FT ECO:0000269|PubMed:21079745, ECO:0000269|PubMed:30890567,
FT ECO:0000269|PubMed:7649393, ECO:0000269|PubMed:7659159,
FT ECO:0000269|PubMed:8647436"
FT MUTAGEN 103
FT /note="I->N: In sy66; lethality at embryonic or larval
FT stages. The few surviving adults have defects in head
FT morphogenesis, resulting from defects in the embryonic
FT migration or determination of head hypodermal cells. Male
FT tail spicule defects. Defects in male specific
FT postembryonic blast cell (B cell) fate specification."
FT /evidence="ECO:0000269|PubMed:15579687,
FT ECO:0000269|PubMed:7649393, ECO:0000269|PubMed:7659159"
FT MUTAGEN 120
FT /note="S->N: In ns157; defects in AWC, AFD and ADF axon
FT guidance. Shorter CEP dendrites. Almost abolishes
FT expression of hlh-17 and ptr-10 in dorsal CEPsh glia,
FT whereas nearly half of animals express both these genes in
FT ventral CEPsh glia."
FT /evidence="ECO:0000269|PubMed:18508862"
FT MUTAGEN 149..455
FT /note="Missing: In e1062; lethality at embryonic or larval
FT stages, or adult head epidermal morphological defects. Male
FT tail spicule defects. Anatomy of the anterior ganglion is
FT severely disturbed and expression of unc-86 is abolished in
FT several neurons in the head."
FT /evidence="ECO:0000269|PubMed:15579687,
FT ECO:0000269|PubMed:8647436"
FT MUTAGEN 160..455
FT /note="Missing: In ot292; defective dopaminergic neuron
FT specification."
FT /evidence="ECO:0000269|PubMed:21079745"
FT MUTAGEN 228..455
FT /note="Missing: In we4; lethality at embryonic or larval
FT stages, or adult head epidermal morphological defects."
FT /evidence="ECO:0000269|PubMed:15579687"
FT MUTAGEN 229..232
FT /note="IESL->TESH: In e1796; adult male tail ray morphology
FT defects. Some mutants show defects in gonadal distal tip
FT cell (DTC) migration. Cell fate transformations of head
FT lateral epidermis in some animals. About 50% of individuals
FT have reduced expression of the integrin alpha pat-2.
FT Abolishes expression of hlh-17 in both dorsal and ventral
FT CEPsh glia."
FT /evidence="ECO:0000269|PubMed:15579687,
FT ECO:0000269|PubMed:17606640, ECO:0000269|PubMed:18508862"
FT MUTAGEN 262..455
FT /note="Missing: In ju468; lethality at embryonic or larval
FT stages, or adult head epidermal morphological defects.
FT Severely defective function of chemosensory BAG neurons.
FT Normal BAG sensory neuron fate, but reduced expression of
FT neuropeptide flp-17 in the BAG neurons. Reduced expression
FT of receptor-type guanylyl cyclase gcy-9. Expression of hlh-
FT 17 abolished in both dorsal and ventral CEPsh glia."
FT /evidence="ECO:0000269|PubMed:15579687,
FT ECO:0000269|PubMed:18508862, ECO:0000269|PubMed:30890567"
FT MUTAGEN 338..455
FT /note="Missing: In ot266; defective dopaminergic neuron
FT specification. Normal BAG sensory neuron fate, but reduced
FT expression of neuropeptide flp-17 in the BAG neurons."
FT /evidence="ECO:0000269|PubMed:21079745,
FT ECO:0000269|PubMed:30890567"
SQ SEQUENCE 455 AA; 51061 MW; 70D75A22A6739C57 CRC64;
MSDAGHTGVN QLGGVFVNGR PLPDATRQRI VDLAHKGCRP CDISRLLQVS NGCVSKILCR
YYESGTIRPR AIGGSKPRVA TSDVVEKIED YKRDQPSIFA WEIRDKLLAD NICNNETIPS
VSSINRVLRN LAAKKEQVTM QTELYDRIRI VDNFPYNSSW YGQWPIPMNG AVGLNPFVPA
PLIEPKTEGE FEKDEDQKPP TEPEDDAAAR MRLKRKLQRN RTSFTQVQIE SLEKEFERTH
YPDVFARERL AQKIQLPEAR IQVWFSNRRA KWRREEKMRN KRSSGTMDSS LSNGTPTPTP
GSVTGSNMTN PIGSPASTPN RFPSNNSANL PTTNFVPQTS QMYAGLSQPA MDPYSFGIAN
GFSMAPYPQV TDFQPHHMFQ GRSPYDFPYP RMPTNGHGFQ QSMSPATTAV GDIPTLSSGM
SLPVSAVLNS IDPSLTHSQM HELSDLTQEH YWRPQ
